Biomod/2011/Caltech/DeoxyriboNucleicAwesome/Protocols: Difference between revisions
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=== SPEX protocol === | === SPEX protocol === | ||
1. Turn on the lamp (back side). Wait for 30 min | * 1. Turn on the lamp (back side). Wait for 30 min | ||
Bottom big on/off button is for the machine. It is always on. | Bottom big on/off button is for the machine. It is always on. | ||
Top small one is for the lamp. If there’s somebody who will use the SPEX tomorrow, then do not turn off the lamp after the experiment. | Top small one is for the lamp. If there’s somebody who will use the SPEX tomorrow, then do not turn off the lamp after the experiment. | ||
2. Turn on the computer if it’s not on. | * 2. Turn on the computer if it’s not on. | ||
3. Software: Instrument control center | * 3. Software: Instrument control center | ||
Default: Click OK | Default: Click OK | ||
4. Temperature set up | * 4. Temperature set up | ||
Click the third square icon called “Visual setup” | Click the third square icon called “Visual setup” | ||
Click the middle square | Click the middle square | ||
Temperature control icon: Turn on and Set to 25 degreeC | Temperature control icon: Turn on and Set to 25 degreeC | ||
Choose T bath (internal). NOT Probe (external). | Choose T bath (internal). NOT Probe (external). | ||
5. Wash the cuvettes. | * 5. Wash the cuvettes. | ||
Be careful! | Be careful! | ||
Hold only top or bottom. | Hold only top or bottom. | ||
Line 32: | Line 32: | ||
While you wash, you can touch the side. Hold it in a stable way. | While you wash, you can touch the side. Hold it in a stable way. | ||
Let it dry (upside down) | Let it dry (upside down) | ||
6. Dry the cuvettes using lens paper. | * 6. Dry the cuvettes using lens paper. | ||
7. Click second square icon called “real time display”. | * 7. Click second square icon called “real time display”. | ||
High voltage: HV on, S 950, T 950 | High voltage: HV on, S 950, T 950 | ||
Bandpass : Set 2 for everything | Bandpass : Set 2 for everything | ||
8. Click the last square icon called “run CWA”. | * 8. Click the last square icon called “run CWA”. | ||
Open a file called “ROX_CWA” from e:\SPEX DATA\biomod | Open a file called “ROX_CWA” from e:\SPEX DATA\biomod | ||
Optional mode: kinetics, Integration time: 10s | Optional mode: kinetics, Integration time: 10s | ||
Define: Repeat acquisition: 1 min (minimum) for total of 180 min (3 hours) | Define: Repeat acquisition: 1 min (minimum) for total of 180 min (3 hours) | ||
9. Mixing: Hold top. 350ul of 1000ul pipette all the way in. Mix it for 30 times. | * 9. Mixing: Hold top. 350ul of 1000ul pipette all the way in. Mix it for 30 times. | ||
10. Put the cuvettes. Logo faces out! | * 10. Put the cuvettes. Logo faces out! | ||
11. Start acquisition. Run sample. | * 11. Start acquisition. Run sample. | ||
Range of the real data should not exceed 1 million (1000,000) | Range of the real data should not exceed 1 million (1000,000) | ||
Number we see is S/R will be (100 million) | Number we see is S/R will be (100 million) | ||
12. Add more samples in the middle of SPEX experiment | * 12. Add more samples in the middle of SPEX experiment | ||
When the status is “waiting”, click “stop”. | When the status is “waiting”, click “stop”. | ||
Don’t click anything else. | Don’t click anything else. | ||
Add and mix. After you put the cuvettes inside the box, click “no”. | Add and mix. After you put the cuvettes inside the box, click “no”. | ||
13. Save the data | * 13. Save the data | ||
When the status is “waiting”, click “stop”. | When the status is “waiting”, click “stop”. | ||
Click “yes” | Click “yes” | ||
Line 55: | Line 55: | ||
Check if the data is properly saved: Check the last data. | Check if the data is properly saved: Check the last data. | ||
* If you want to see the reference, go to options: view option and display raw R. | * If you want to see the reference, go to options: view option and display raw R. | ||
===How to Open SPEX Data in MATLAB=== | ===How to Open SPEX Data in MATLAB=== |
Revision as of 15:35, 12 October 2011
Tuesday, April 23, 2024
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ProtocolsThings that need to be written
=SPEX protocol
Bottom big on/off button is for the machine. It is always on. Top small one is for the lamp. If there’s somebody who will use the SPEX tomorrow, then do not turn off the lamp after the experiment.
Default: Click OK
Click the third square icon called “Visual setup” Click the middle square Temperature control icon: Turn on and Set to 25 degreeC Choose T bath (internal). NOT Probe (external).
Be careful! Hold only top or bottom. DW (10 times) 70% ethanol (twice) DW (5 times) 70% ethanol (once) While you wash, you can touch the side. Hold it in a stable way. Let it dry (upside down)
High voltage: HV on, S 950, T 950 Bandpass : Set 2 for everything
Open a file called “ROX_CWA” from e:\SPEX DATA\biomod Optional mode: kinetics, Integration time: 10s Define: Repeat acquisition: 1 min (minimum) for total of 180 min (3 hours)
Range of the real data should not exceed 1 million (1000,000) Number we see is S/R will be (100 million)
When the status is “waiting”, click “stop”. Don’t click anything else. Add and mix. After you put the cuvettes inside the box, click “no”.
When the status is “waiting”, click “stop”. Click “yes” Save data in two forms (DWA datafile, multi~ test) Check if the data is properly saved: Check the last data.
How to Open SPEX Data in MATLAB
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