Progress 1: System Design
Design of the overall system
Overall domain level design is illustrated in figure 1. Following abbreviation will be frequently used: walker [W], walker inhibitor [WI], track 1 [TR1], probe for track 1 [PTR1], track 2 [TR2], probe for track 2 [PTR2], cargo 1 [C1], cargo attacher [CA], probe for cargo attacher [PCA], cargo goal inhibitor [CGI], cargo goal 1 [CG1], probe for cargo goal [PCG], walker goal [WG], and probe for walker goal [PWG].
With our overall design in mind, we must design DNA sequences, down to the base level, which undergo the interactions that we desire, without forming secondary structures and binding in unintended ways. We approach this through a combination of pre-generated noninteracting sequences, and trial-and-error design using NUPACK simulation software.
Also, Random walking playground and cargo sorting playground were designed using a rectangular orgiami.
Design of the experiments
We used three different experimental techniques, and here is the design of each experiments.
1. Before constructing our origami and observing how it behaves, we run a large number of experiments observable through Gel Electrophoresis to verify that many of our mechanisms behave as we expect them to.
2. Various DNA strands are tagged with fluorophores and quenchers in order to investigate different mechanisms more directly, both in solution and on origami.
3. Individual system is observed directly under AFM.