Biomod/2011/Caltech/DeoxyriboNucleicAwesome/AFM Experiments

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Wednesday, April 24, 2024

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AFM Imaging

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We started by trying to form a regular rectangle to make sure our protocols worked as expected.
We started by trying to form a regular rectangle to make sure our protocols worked as expected.

We reformed the regular rectangle using our modified staple strands to show that the probes don't interfere with the binding in the rectangle.
We reformed the regular rectangle using our modified staple strands to show that the probes don't interfere with the binding in the rectangle.

We showed that our probes bind in the proper location by adding track strands and noting where they bind on the origami.
We showed that our probes bind in the proper location by adding track strands and noting where they bind on the origami.

We attempted to insert our walker at the beginning of the track for the first time, but we can't see it on the AFM images. This could be due to any one of a number of issues.
We attempted to insert our walker at the beginning of the track for the first time, but we can't see it on the AFM images. This could be due to any one of a number of issues.

We first see what our walker should look like by adding another control to our origami. The new bright dot is the protein streptavidin.
We first see what our walker should look like by adding another control to our origami. The new bright dot is the protein streptavidin.

After some work, we can now see walkers on the surface of our origami.
After some work, we can now see walkers on the surface of our origami.