Biomod/2011/Aarhus/DanishNanoArtists - Revision history

Steffen Sparvath: /* Octahedral RNA origami for simultaneous drug delivery and gene knockdown */

2011-11-03T01:31:22Z

Octahedral RNA origami for simultaneous drug delivery and gene knockdown

←Older revision		Revision as of 01:31, 3 November 2011
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	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Gel_electrophoresis\|PAGE]]		[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Gel_electrophoresis\|PAGE]]
	and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a		and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a
-	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Dual_Luciferace_assay\|dual Luciferase assay]]. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.	+	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Dual_Luciferace_assay\|dual Luciferase assay]]. We used a DNA/RNA hybrid to characterize opening of the structure by
		+	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#FRET\|FRET measurements]]. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.

	<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>		<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>

Steffen Sparvath: /* Octahedral RNA origami for simultaneous drug delivery and gene knockdown */

2011-11-03T01:29:51Z

Octahedral RNA origami for simultaneous drug delivery and gene knockdown

←Older revision		Revision as of 01:29, 3 November 2011
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	towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using		towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using
	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Gel_electrophoresis\|PAGE]]		[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Gel_electrophoresis\|PAGE]]
-	and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.	+	and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a
		+	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Dual_Luciferace_assay\|dual Luciferase assay]]. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.

	<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>		<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>

Steffen Sparvath: /* Octahedral RNA origami for simultaneous drug delivery and gene knockdown */

2011-11-03T01:21:37Z

Octahedral RNA origami for simultaneous drug delivery and gene knockdown

←Older revision		Revision as of 01:21, 3 November 2011
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	enzyme the structure can release up to 5		enzyme the structure can release up to 5
	[[Biomod/2011/Aarhus/DanishNanoArtists/Project#Gene_knockdown_using_the_RNAi_pathway\|siRNAs]]		[[Biomod/2011/Aarhus/DanishNanoArtists/Project#Gene_knockdown_using_the_RNAi_pathway\|siRNAs]]
-	towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.	+	towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using
		+	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Gel_electrophoresis\|PAGE]]
		+	and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.

	<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>		<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>

Steffen Sparvath: /* Octahedral RNA origami for simultaneous drug delivery and gene knockdown */

2011-11-03T01:19:47Z

Octahedral RNA origami for simultaneous drug delivery and gene knockdown

←Older revision		Revision as of 01:19, 3 November 2011
Line 8:		Line 8:
	An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the		An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the
	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Dicer\|Dicer]]		[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Dicer\|Dicer]]
-	enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.	+	enzyme the structure can release up to 5
		+	[[Biomod/2011/Aarhus/DanishNanoArtists/Project#Gene_knockdown_using_the_RNAi_pathway\|siRNAs]]
		+	towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.

	<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>		<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>

Steffen Sparvath: /* Octahedral RNA origami for simultaneous drug delivery and gene knockdown */

2011-11-03T01:10:38Z

Octahedral RNA origami for simultaneous drug delivery and gene knockdown

←Older revision		Revision as of 01:10, 3 November 2011
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	An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the		An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the
-	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Dicer\|Dicer]] enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.	+	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Dicer\|Dicer]]
		+	enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.

	<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>		<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>

Steffen Sparvath: /* Octahedral RNA origami for simultaneous drug delivery and gene knockdown */

2011-11-03T01:10:10Z

Octahedral RNA origami for simultaneous drug delivery and gene knockdown

←Older revision		Revision as of 01:10, 3 November 2011
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	==Octahedral RNA origami for simultaneous drug delivery and gene knockdown==		==Octahedral RNA origami for simultaneous drug delivery and gene knockdown==

-	An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the Dicer enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.	+	An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the
		+	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Dicer\|Dicer]] enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.

	<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>		<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>

Steffen Sparvath: /* Octahedral RNA origami for simultaneous drug delivery and gene knockdown */

2011-11-03T00:37:52Z

Octahedral RNA origami for simultaneous drug delivery and gene knockdown

←Older revision		Revision as of 00:37, 3 November 2011
Line 8:		Line 8:
	An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the Dicer enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.		An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the Dicer enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.

-		+	<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>


	[[Image:Octahedron.png\|750px\|center]]		[[Image:Octahedron.png\|750px\|center]]

-
-
-
-	~~<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>~~

Steffen Sparvath: /* Octahedral RNA origami for simultaneous drug delivery and gene knockdown */

2011-11-03T00:37:14Z

Octahedral RNA origami for simultaneous drug delivery and gene knockdown

←Older revision		Revision as of 00:37, 3 November 2011
Line 8:		Line 8:
	An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the Dicer enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.		An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the Dicer enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.

-	~~<center>[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]</center>~~


-	~~[[Image:Octahedron.png\|800px\|center]]~~

		+	[[Image:Octahedron.png\|750px\|center]]
		+
		+
		+
		+
		+	<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>

Steffen Sparvath: /* Octahedral RNA origami for simultaneous drug delivery and gene knockdown */

2011-11-02T18:14:13Z

Octahedral RNA origami for simultaneous drug delivery and gene knockdown

←Older revision		Revision as of 18:14, 2 November 2011
Line 8:		Line 8:
	An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the Dicer enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.		An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the Dicer enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.

-	<center>[[Biomod/2011/Aarhus/DanishNanoArtists/Idea\|To see our YouTube video click here]]</center>	+	<center>[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]</center>

Steffen Sparvath: /* Octahedral RNA origami for simultaneous drug delivery and gene knockdown */

2011-11-02T16:01:29Z

Octahedral RNA origami for simultaneous drug delivery and gene knockdown

←Older revision		Revision as of 16:01, 2 November 2011
Line 7:		Line 7:

	An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the Dicer enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.		An RNA octahedron was designed that can be folded through scaffolded RNA origami on any RNA sequence. By designing specific recognition sites for the Dicer enzyme the structure can release up to 5 siRNAs towards the same gene resulting in effective knockdown. In addition, the structure can release drug molecules either in response to a miRNA signal or the Dicer cleavage. Here we characterise the structure of the RNA octahedron using PAGE and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a dual Luciferase assay. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.
		+
		+	<center>[[Biomod/2011/Aarhus/DanishNanoArtists/Idea\|To see our YouTube video click here]]</center>

←Older revision		Revision as of 01:31, 3 November 2011
Line 13:		Line 13:
	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Gel_electrophoresis\|PAGE]]		[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Gel_electrophoresis\|PAGE]]
	and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a		and biophysical techniques, and the function by specific cleavage of the structure by the Dicer enzyme and gene knockdown using a
-	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Dual_Luciferace_assay\|dual Luciferase assay]]. We used a DNA/RNA hybrid to characterize opening of the structure by FRET measurements. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.	+	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#Dual_Luciferace_assay\|dual Luciferase assay]]. We used a DNA/RNA hybrid to characterize opening of the structure by
		+	[[Biomod/2011/Aarhus/DanishNanoArtists/Project/Methods#FRET\|FRET measurements]]. Our project demonstrates that RNA nano-structures can be designed as multipurpose devices for both stability, drug release and using the scaffold as the active therapeutic agent.

	<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>		<center><big>'''[[Biomod/2011/Aarhus/DanishNanoArtists/Idea#Goals\|to see our YouTube video click here]]'''</big></center>