BioSysBio:abstracts/2007/Claudia Choi

Analysis of metabolome data by a maximum likelihood approach

Author(s): Claudia Choi¹, Claudia Hundertmark², Bernhard Thielen³, Beatrice Benkert¹, Richard Münch¹, Max Schobert¹, Dietmar Schomburg³, Dieter Jahn¹, Frank Klawonn⁴

Affiliations: ¹Institut für Mikrobiologie, Technische Universitält Braunschweig, Germany; ²Helmholtz-Zentrum für Infektionsforschung GmbH, Germany; ³Institut für Biochemie, Universität zu Köln, Germany; ⁴Fachbereich Informatik, Fachhochschule Wolfenbüttel, Germany

Contact: email: c.choi AT tu-bs.de

Keywords: 'metabolomics'

Abstract

Metabolomics emerges as one key aspect of systems biology, since quantifying the dynamic set of metabolites reveals the effect of altered gene expression and protein pattern and thus complements transcriptomics and proteomics. By high-throughput techniques, such as measuring metabolites by gas chromatography-mass spectrometry (GC-MS), enormous data amounts are produced, that need to be analysed. At present, a variety of methods are available for cluster analysis of metabolome data.
Our maximum likelihood approach identifies significantly altered metabolites between Pseudomonas aeruginosa samples grown under different conditions and measured by GC-MS. P. aeruginosa is a versatile soil bacterium and an important opportunistic pathogen causing persistent infection in immunocompromised patients. This statistical approach estimates the inherent noise of the samples and thereby evaluates the significance of altered metabolite composition. Identified key metabolites with significantly altered pattern under different conditions, will be interesting for further investigation of the metabolic network and flux analysis.