BioMicroCenter:Varioskan: Difference between revisions
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' | == PLATE READER == | ||
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! Instrument || ThermoFisher Varioskan | |||
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| TYPE || WALKUP - MIT only | |||
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| UNIT || Per hour in 10' increments | |||
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| FORMAT || 96w/394w | |||
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| FLUOROMETRY | |||
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* 6-1536w | |||
* Excite: 200-1000nm | |||
* Emission: 270-840nm | |||
* Sensitivity: <0.4fmol fluorescein/w | |||
* Dynamic Range: >6 decades | |||
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| LUMINOMETRY | |||
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* 6-384w spectral scan | |||
* 360-670nm | |||
* 270-840nm spectral scan | |||
* Sensitivity: < amol ATP/w | |||
* Dynamic Range: >7 decades | |||
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| PHOTOMETRY <BR> ABSORBANCE | |||
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* 6-384w | |||
* Wavelength: 200-1000nm | |||
* Sensitivity: 0.003Abs +/-2% | |||
* Dynamic Range: 0-4Abs @450nm | |||
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| SIGNUP || [https://mit.ilabsolutions.com/equipment/show/262715/?tab=schedule ilabs] | |||
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| NEW USERS || New users should request training by emailing biomicro@mit.edu | |||
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| DONATED BY || Prof. Jeroen Saeij. | |||
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The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the [[BioMicroCenter:Tecan_Freedom_Evo|Tecan EVO]], the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in '''68-322'''. <BR><BR> | |||
''The Varioskan supports:'' | |||
* 3 onboard liquid dispensers | * 3 onboard liquid dispensers | ||
* 1ul – 10ml dispensing volumes | * 1ul – 10ml dispensing volumes | ||
* | * Temperature control from ambient + 20C – 45C | ||
* On board orbital shaker | * On board orbital shaker | ||
The varioskan is used routinely in the BioMicro Center to measure the concentration of Illumina libraries using SYBRgreen. The Varioskan will also be used to measure concentrations of oligos from Dr.Oligo.<br><br> | |||
''Suggested Assays:'' | |||
* Apoptosis assays | * Apoptosis assays | ||
* Ca2+ flux assays | * Ca2+ flux assays | ||
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* Cellular assays | * Cellular assays | ||
* Cytotoxicity and ADMETox | * Cytotoxicity and ADMETox | ||
* DNA concentration | |||
* ELISA/FIA/TRF-ELISA assays | * ELISA/FIA/TRF-ELISA assays | ||
* Enzyme kinetic studies | * Enzyme kinetic studies | ||
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* Signal transduction | * Signal transduction | ||
* Tryptophan and tyrosine UV fluorescence | * Tryptophan and tyrosine UV fluorescence | ||
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[[Image:BioMicroCenter_Varioskan.jpg|right]]<BR> | |||
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Latest revision as of 09:38, 6 September 2023
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PLATE READER
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The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the Tecan EVO, the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in 68-322. The Varioskan supports:
The varioskan is used routinely in the BioMicro Center to measure the concentration of Illumina libraries using SYBRgreen. The Varioskan will also be used to measure concentrations of oligos from Dr.Oligo.
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