BioMicroCenter:Varioskan: Difference between revisions
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The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the [[BioMicroCenter:Tecan_Freedom_Evo|Tecan EVO]], the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in '''68-252d'''. Please speak with '''Kevin Thai''' at the BioMicro Center about training if you wish to use the machine. | The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the [[BioMicroCenter:Tecan_Freedom_Evo|Tecan EVO]], the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in '''68-252d'''. Please speak with '''Kevin Thai''' at the BioMicro Center about training if you wish to use the machine. | ||
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* On | * On September 15th, 2010, the Varioskan will be relocated to 68-316. At that time a $10/plate charge will be implemented to cover the charge of the service contract. Plates requiring minimal time on the machine (<10 minutes) can be scanned without charge but may not be scheduled on the calender and must give priority to scheduled users. We will post an announcement before we plan to move the instrument so all users are aware of the move ahead of time. | ||
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Revision as of 06:57, 14 September 2010
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Varioskan Flash Reader
The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the Tecan EVO, the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in 68-252d. Please speak with Kevin Thai at the BioMicro Center about training if you wish to use the machine.
- On September 15th, 2010, the Varioskan will be relocated to 68-316. At that time a $10/plate charge will be implemented to cover the charge of the service contract. Plates requiring minimal time on the machine (<10 minutes) can be scanned without charge but may not be scheduled on the calender and must give priority to scheduled users. We will post an announcement before we plan to move the instrument so all users are aware of the move ahead of time.
Schedule time on the Varioskan
Specifications
| Fluorometry | Luminometry | Photometry | |
|---|---|---|---|
| Plate Density: | 6-1536 well | 6-1536 well 6-386 well spectral scan |
6-384 well |
| Wavelength: | Ex: 200-1000 nm Em: 270-840 nm |
360-670 nm 270-840 nm spectral scan |
200-1000 nM |
| Sensitivity: | <0.4 fmol flourescein/well | < amol ATP/well | +/- 2% or 0.003 Abs |
| Dynamic range: | > 6 decades | > 7 decades | 0-4 Abs @ 450 nm +/-2% |
Features:
- 3 onboard liquid dispensers
- 1ul – 10ml dispensing volumes
- Temperature control from ambient + 4C – 45C
- On board orbital shaker
Suggested Applications
- Apoptosis assays
- Ca2+ flux assays
- Cell proliferation
- Cellular assays
- Cytotoxicity and ADMETox
- DNA concentration
- ELISA/FIA/TRF-ELISA assays
- Enzyme kinetic studies
- Europium assays
- FRET assays
- TR-FRET assays
- BRET assays
- GPCR assays
- Ion channel assays
- Kinase assays
- Multilabel assays
- Reporter gene assays
- Signal transduction
- Tryptophan and tyrosine UV fluorescence
