BioMicroCenter:Tecan Freedom Evo
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** All users must be trained before being allowed to use the Tecans **
The BioMicro Center currently hosts two Tecan Freedom EVO 150s named Johnny5 and B9. Johnny5 is optimized for cherry picking applications while B9 is optimized for screening.
JOHNNY 5
"Johnny5" is the BioMicro Center robot optimized for cherry picking. It is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting.
ARMS
Johnny 5 has the following arms:
- LiHa (Liquid handler): 4 fixed conductivity sensing tips. 1-500 µl
- MCA (Multichannel Arm): 96 Tip head uses disposable tips (50 or 200 µl)
CARRIERS
Johnny 5's deck is configured with the following carriers.
- 1 fixed tip washer.
- 3 15ml tube carriers.
- 2 temperature controlled carriers (holds 6 plates)
- 3 non-temperature controlled carriers (hold 10 plates)
- 3 spots for tops.
SCHEDULE TIME ON JOHNNY 5 Note, time must be booked in 2 hour increments.
B9
"B9" is the BioMicro Center EVO150 optimized for high throughput screening. B9 has a 96 tip head for rapid pipetting and a robotic arm for moving plates around the deck. The robot has 4 plate stackers which can be used to add or remove plates during a run.
ARMS
B9 has the following arms:
- MCA (Multichannel Arm): 96 Tip head uses disposable tips (50 or 200 µl)
- RoMa (Remote Manipulator): Robotic are to move plates around.
CARRIERS
B9 has the following Carriers:
- 1 rack for 3 non-SBS tip racks
- 1 rack for 2 stacks of nested tips with chute
- 3 racks for SBS tips (12 spots total)
- TeVac vacuum manifold
- Vortexer (Te-Shake)
- 2 on deck racks for short plates
- Shaking Incubator
- 4 Stackers (TeStack)
Schedule time on B9 Note, time must be booked in 2 hour increments.
EVOWARE
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The Tecan EVO currently runs using the EVOware software package. EVOware can be run in either of two modes: directly connected to the Tecan EVO to allow control of the robot, or on your desktop computer in a simulation mode. Simulation mode is extremely useful in that it allows the user to pre-design the steps in their program. All users must download EVOware and design their protocols to be brought to the EVO.
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LABWARE
LABWARE
Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center will stock a standard set of labware that will be included in the "Base.xsd" file.
LIST OF LABWARE - We are currently evaluating different types of labware. If you are interested in having a particular piece of labware, please make sure to edit the list.
LOGISTICS
SCHEDULING
Access to the Tecan liquid handling robots is available in 2 hour blocks. You can schedule use of either robot by clicking on the link below.
- Tecan Evo - -
COST
In order to defray the costs of the annual service contract, each 2 hour session on the EVO will be charged $50.. Tips and 96 well plates are available for purchase from the BioMicro center or obtained from your own lab. Additionally, training is required for all new users. A training session is $100
