BioMicroCenter:Tecan Freedom Evo - Revision history

Stuart S. Levine at 21:25, 20 May 2012

2012-05-20T21:25:23Z

←Older revision		Revision as of 21:25, 20 May 2012
Line 1:		Line 1:
	{{BioMicroCenter}}		{{BioMicroCenter}}
-	~~''' All users must be trained before being allowed to use the Tecans '''~~

		+	''' All users must be trained before being allowed to use the Tecans '''<BR>
	The BioMicro Center currently hosts two Tecan Freedom EVO 150s named Johnny5 and B9. Johnny5 is optimized for cherry picking applications while B9 is optimized for screening. The Tecan's in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+ a new High-Throughput core is being developed by the [http://ki.mit.edu/sbc Koch Institute.]		The BioMicro Center currently hosts two Tecan Freedom EVO 150s named Johnny5 and B9. Johnny5 is optimized for cherry picking applications while B9 is optimized for screening. The Tecan's in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+ a new High-Throughput core is being developed by the [http://ki.mit.edu/sbc Koch Institute.]

Stuart S. Levine at 21:24, 20 May 2012

2012-05-20T21:24:58Z

←Older revision		Revision as of 21:24, 20 May 2012
Line 1:		Line 1:
	{{BioMicroCenter}}		{{BioMicroCenter}}
-	All users must be trained before being allowed to use the Tecans	+	''' All users must be trained before being allowed to use the Tecans '''

	The BioMicro Center currently hosts two Tecan Freedom EVO 150s named Johnny5 and B9. Johnny5 is optimized for cherry picking applications while B9 is optimized for screening. The Tecan's in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+ a new High-Throughput core is being developed by the [http://ki.mit.edu/sbc Koch Institute.]		The BioMicro Center currently hosts two Tecan Freedom EVO 150s named Johnny5 and B9. Johnny5 is optimized for cherry picking applications while B9 is optimized for screening. The Tecan's in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+ a new High-Throughput core is being developed by the [http://ki.mit.edu/sbc Koch Institute.]

Stuart S. Levine: /* LABWARE */

2012-05-20T21:22:05Z

LABWARE

←Older revision		Revision as of 21:22, 20 May 2012
Line 61:		Line 61:
	Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center stocks several types of labware which are available to users:		Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center stocks several types of labware which are available to users:

-	{\| border=1 ~~align="left"~~	+	{\| border=1
	! TIPS		! TIPS
	! PLATES		! PLATES
Line 71:		Line 71:
	* 200ul standard		* 200ul standard
	* 200ul filter, low bind, sterile		* 200ul filter, low bind, sterile
-	\|	+	\|
	* 96well Axygen full skirted PCR plate (PCR-96-FS-C)		* 96well Axygen full skirted PCR plate (PCR-96-FS-C)
	* 96well Axygen RT-PCR plates		* 96well Axygen RT-PCR plates
	* 384well Roche RT-PCR plates		* 384well Roche RT-PCR plates
	* Large reservoirs		* Large reservoirs
		+	\|-
	\|}		\|}
	<BR>		<BR>

Stuart S. Levine: /* LABWARE */

2012-05-20T21:21:00Z

LABWARE

←Older revision		Revision as of 21:21, 20 May 2012
Line 77:		Line 77:
	* Large reservoirs		* Large reservoirs
	\|}		\|}
		+	<BR>

	== SERVICES ==		== SERVICES ==

Stuart S. Levine: /* LABWARE */

2012-05-20T21:20:19Z

LABWARE

←Older revision		Revision as of 21:20, 20 May 2012
Line 61:		Line 61:
	Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center stocks several types of labware which are available to users:		Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center stocks several types of labware which are available to users:

-	=== TIPS ~~(MCA) ===~~	+	{\| border=1 align="left"
		+	! TIPS
		+	! PLATES
		+	\|-
		+	\|
	* 50ul standard		* 50ul standard
	* 50ul filter,low bind, sterile		* 50ul filter,low bind, sterile
Line 67:		Line 71:
	* 200ul standard		* 200ul standard
	* 200ul filter, low bind, sterile		* 200ul filter, low bind, sterile
-		+	\|
-	~~=== PLATES ===~~	+
	* 96well Axygen full skirted PCR plate (PCR-96-FS-C)		* 96well Axygen full skirted PCR plate (PCR-96-FS-C)
	* 96well Axygen RT-PCR plates		* 96well Axygen RT-PCR plates
	* 384well Roche RT-PCR plates		* 384well Roche RT-PCR plates
		+	* Large reservoirs
		+	\|}

	== SERVICES ==		== SERVICES ==

Stuart S. Levine at 21:17, 20 May 2012

2012-05-20T21:17:28Z

←Older revision		Revision as of 21:17, 20 May 2012
Line 2:		Line 2:
	All users must be trained before being allowed to use the Tecans		All users must be trained before being allowed to use the Tecans

-	The BioMicro Center currently hosts two Tecan Freedom EVO 150s named Johnny5 and B9. Johnny5 is optimized for cherry picking applications while B9 is optimized for screening.	+	The BioMicro Center currently hosts two Tecan Freedom EVO 150s named Johnny5 and B9. Johnny5 is optimized for cherry picking applications while B9 is optimized for screening. The Tecan's in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+ a new High-Throughput core is being developed by the [http://ki.mit.edu/sbc Koch Institute.]

	==JOHNNY 5==		==JOHNNY 5==
Line 18:		Line 18:
	* 1 fixed tip washer.		* 1 fixed tip washer.
	* 3 15ml tube carriers.		* 3 15ml tube carriers.
-	* 2 temperature controlled carriers (holds 6 plates)	+	* 2 temperature controlled SBS carriers (holds 6 plates)
-	* 3 non-temperature controlled carriers (hold 10 plates)	+	* 3 non-temperature controlled SBS carriers (hold 10 plates)
-	* 3 spots for ~~tops~~.	+	* 3 spots for tips.
		+	* 4 racks for 1.5ml tubes (24 each)

	[[BioMicroCenter:Tecan_CAL\| ''' SCHEDULE TIME ON JOHNNY 5 ''']]		[[BioMicroCenter:Tecan_CAL\| ''' SCHEDULE TIME ON JOHNNY 5 ''']]
Line 27:		Line 28:
	== B9 ==		== B9 ==
	[[Image:BMC_B9_robot.png\|right\|100px]]		[[Image:BMC_B9_robot.png\|right\|100px]]
-	"B9" is the BioMicro Center EVO150 optimized for high throughput screening. B9 has a 96 tip head for rapid pipetting and a robotic arm for moving plates around the deck. The robot has 4 plate stackers which can be used to add or remove plates during a run.	+	"B9" is the BioMicro Center EVO150 optimized for high throughput screening. B9 has a 96 tip head for rapid pipetting and a robotic arm for moving plates around the deck. The robot has 4 plate stackers which can be used to add or remove plates during a run. B9 was donated to the BioMicro Center by [http://gilbertlab.mit.edu/gilbertlabpeople.html Dr. Wendy Gilbert] through a small instrumentation grant from the NIH.

	===ARMS===		===ARMS===
Line 58:		Line 59:

	== LABWARE ==		== LABWARE ==
		+	Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center stocks several types of labware which are available to users:

		+	=== TIPS (MCA) ===
		+	* 50ul standard
		+	* 50ul filter,low bind, sterile
		+	* 50ul nested stack
		+	* 200ul standard
		+	* 200ul filter, low bind, sterile

		+	=== PLATES ===
		+	* 96well Axygen full skirted PCR plate (PCR-96-FS-C)
		+	* 96well Axygen RT-PCR plates
		+	* 384well Roche RT-PCR plates

		+	== SERVICES ==

		+	Use of the BioMicro Center Tecans can either be supervised or full-service.

-	~~</pre>~~	+	=== Supervised ===
		+	In supervised use, the BioMicro Center will provide training for a new user. The user is then approved to work on the Tecans on their own. Newer users should work when BioMicro Center staff are available to answer questions. Usage of the robots for practice is not charged but has lowest priority. Practice time must be booked on the calender and indicated as practice. Practice time must also be logged on the time sheet next to the robot. A valid cost object number must be on record in the BioMicro Center and consumables are still charged. Once the user is using real samples, time is charged at our current rate found on the [[BioMicroCenter:Pricing\|pricing page]].

-	~~== LABWARE ==~~	+	'''FAILURE TO FOLLOW BMC POLICIES ON SAFETY OR USAGE OF THE ROBOTS WILL RESULT IN REMOVAL OF YOUR ACCESS TO THE BIOMICRO CENTER'''
-	Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center will stock a standard set of labware that will be included in the "Base.xsd" file.	+
-		+
-	~~[[BioMicroCenter:EVOlabware \| LIST~~ OF ~~LABWARE]] - We are currently evaluating different types of labware. If you are interested in having a particular piece of labware, please make sure to edit the list.~~	+
-		+
-	~~== LOGISTICS ==~~	+
-		+
-		+
-		+
-	~~=== SCHEDULING ===~~	+
-	~~Access to the Tecan liquid handling robots is available in 2 hour blocks. You can schedule use of either robot by clicking on the link below.~~	+
-	* ~~[[BioMicroCenter:Tecan_Freedom_Evo\|Tecan Evo]] - -~~	+

-	=== ~~[[BioMicroCenter:Pricing\|COST]]~~ ===	+	=== Full Service ===
-	In ~~order to defray the costs of the annual service contract~~, ~~each 2 hour session on~~ the ~~EVO~~ will be ~~charged ''~~'~~$50~~.~~'''~~. ~~Tips~~ and ~~96 well plates are available~~ for ~~purchase from~~ the ~~BioMicro center or obtained from your own lab~~. ~~Additionally, training~~ is ~~required for all new users~~. ~~A training session is $100~~	+	In Full Service use, the BioMicro Center will be responsible for creating scripts, labware, and liquid classes to handle a user's protocol. Once completed, the user will be trained to run the protocol. Setup time will be estimated at a preliminary meeting and a cost and timeline will be estimated for the project. Once the protocol is set up, standard usage rates apply. Rates are on the [[BioMicroCenter:Pricing\|pricing page]].

Stuart S. Levine at 20:46, 20 May 2012

2012-05-20T20:46:03Z

←Older revision		Revision as of 20:46, 20 May 2012
Line 26:		Line 26:

	== B9 ==		== B9 ==
		+	[[Image:BMC_B9_robot.png\|right\|100px]]
		+	"B9" is the BioMicro Center EVO150 optimized for high throughput screening. B9 has a 96 tip head for rapid pipetting and a robotic arm for moving plates around the deck. The robot has 4 plate stackers which can be used to add or remove plates during a run.

-	== ~~EVOWARE~~ ==	+	===ARMS===
		+	[[Image:BMC_B9.png\|right\|300px]]
		+	B9 has the following arms:
		+	* MCA (Multichannel Arm): 96 Tip head uses disposable tips (50 or 200 µl)
		+	* RoMa (Remote Manipulator): Robotic are to move plates around.

-	== ~~LABWARE~~ ==	+	===CARRIERS===
		+	B9 has the following Carriers:
		+	* 1 rack for 3 non-SBS tip racks
		+	* 1 rack for 2 stacks of nested tips with chute
		+	* 3 racks for SBS tips (12 spots total)
		+	* [http://www.tecan.com/platform/apps/product/index.asp?MenuID=3416&ID=7010&Menu=1&Item=21.1.9.26 TeVac vacuum manifold]
		+	* [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1399&ID=973&Menu=1&Item=21.1.9.11 Vortexer (Te-Shake)]
		+	* 2 on deck racks for short plates
		+	* Shaking Incubator
		+	* [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1214&ID=611&Menu=1&Item=21.1.9.13 4 Stackers (TeStack)]

		+	[[BioMicroCenter:Tecan_CAL2\|'''Schedule time on B9''']]
		+	'' Note, time must be booked in 2 hour increments. ''

-		+	== EVOWARE ==
-	~~[[Image:Tn_PR004749.jpg\|right\|97px]]~~	+
-		+
-		+
-	The second EVO150 arrived in the BioMicro Center in June 2010. This robot has several additional features focused on screening 96 and 384 well plates. These include a 96-channel pipette arm, a remote manipulator arm, plate and tip stackers, plate shakers and incubators, etc. For more information about the Tecans, please email [[BioMicroCenter:People\|Stuart Levine]]	+
-		+
-	~~[[Image:BioMicroCenter_Tecan2_Sim.png\|frame\|none\|200px\|left\|alt~~=~~TecanImage\|Simulated view of new Tecan EVO150]]~~	+
-		+
-	=~~= SOFTWARE~~ ==	+
	{\|		{\|
-	\|[[Image:~~BioMicroCenter-EVOware~~.png\|~~200px~~]]	+	\|[[Image:BMC_Evoware.png\|300px]]
	\| The Tecan EVO currently runs using the [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1458&ID=1084&Menu=1&Item=21.7.1 EVOware] software package. EVOware can be run in either of two modes: directly connected to the Tecan EVO to allow control of the robot, or on your desktop computer in a simulation mode. Simulation mode is extremely useful in that it allows the user to pre-design the steps in their program. All users must download EVOware and design their protocols to be brought to the EVO.		\| The Tecan EVO currently runs using the [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1458&ID=1084&Menu=1&Item=21.7.1 EVOware] software package. EVOware can be run in either of two modes: directly connected to the Tecan EVO to allow control of the robot, or on your desktop computer in a simulation mode. Simulation mode is extremely useful in that it allows the user to pre-design the steps in their program. All users must download EVOware and design their protocols to be brought to the EVO.
	<br>		<br>
	* [[BioMicroCenter:EVOware \| Obtain a copy of EVOware]]		* [[BioMicroCenter:EVOware \| Obtain a copy of EVOware]]
	\|}		\|}
-	~~<pre>~~	+
-	~~All programs must be approved by Stuart Levine or Wendy Gilbert before being loaded onto the TECAN.~~	+	== LABWARE ==
		+
		+
		+
		+
		+
	</pre>		</pre>

Line 55:		Line 69:

	[[BioMicroCenter:EVOlabware \| LIST OF LABWARE]] - We are currently evaluating different types of labware. If you are interested in having a particular piece of labware, please make sure to edit the list.		[[BioMicroCenter:EVOlabware \| LIST OF LABWARE]] - We are currently evaluating different types of labware. If you are interested in having a particular piece of labware, please make sure to edit the list.
-
-	~~== HARDWARE ==~~
-	* 4 channel liquid handling arm (LiHa)
-	* 96 channel multichannel arm (MCA)
-	* Vacuum Manifold (TVac)
-	* 2 thermal control plate racks (4C - ??C)
-	* 2 ambient plate racks

	== LOGISTICS ==		== LOGISTICS ==
Line 69:		Line 76:
	=== SCHEDULING ===		=== SCHEDULING ===
	Access to the Tecan liquid handling robots is available in 2 hour blocks. You can schedule use of either robot by clicking on the link below.		Access to the Tecan liquid handling robots is available in 2 hour blocks. You can schedule use of either robot by clicking on the link below.
-	* [[BioMicroCenter:Tecan_Freedom_Evo\|Tecan Evo]] - - ~~[[BioMicroCenter:Tecan_CAL2\|B9 (RoMa/MCA)]]~~	+	* [[BioMicroCenter:Tecan_Freedom_Evo\|Tecan Evo]] - -

	=== [[BioMicroCenter:Pricing\|COST]] ===		=== [[BioMicroCenter:Pricing\|COST]] ===
	In order to defray the costs of the annual service contract, each 2 hour session on the EVO will be charged '''$50.'''. Tips and 96 well plates are available for purchase from the BioMicro center or obtained from your own lab. Additionally, training is required for all new users. A training session is $100		In order to defray the costs of the annual service contract, each 2 hour session on the EVO will be charged '''$50.'''. Tips and 96 well plates are available for purchase from the BioMicro center or obtained from your own lab. Additionally, training is required for all new users. A training session is $100

Stuart S. Levine: /* JOHNNY 5 */

2012-05-20T20:28:07Z

JOHNNY 5

←Older revision		Revision as of 20:28, 20 May 2012
Line 6:		Line 6:
	==JOHNNY 5==		==JOHNNY 5==
	[[Image:BMC_Johnny5_robot.jpg\|right\|100px]]		[[Image:BMC_Johnny5_robot.jpg\|right\|100px]]
-	"Johnny5" is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting.	+	"Johnny5" is the BioMicro Center robot optimized for cherry picking. It is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting.

	===ARMS===		===ARMS===

Stuart S. Levine at 20:26, 20 May 2012

2012-05-20T20:26:19Z

←Older revision		Revision as of 20:26, 20 May 2012
Line 1:		Line 1:
	{{BioMicroCenter}}		{{BioMicroCenter}}
		+	All users must be trained before being allowed to use the Tecans
		+
		+	The BioMicro Center currently hosts two Tecan Freedom EVO 150s named Johnny5 and B9. Johnny5 is optimized for cherry picking applications while B9 is optimized for screening.
		+
		+	==JOHNNY 5==
		+	[[Image:BMC_Johnny5_robot.jpg\|right\|100px]]
		+	"Johnny5" is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting.
		+
		+	===ARMS===
		+	[[Image:BMC_Johnny5.jpg\|right\|300px]]
		+	Johnny 5 has the following arms:
		+	* LiHa (Liquid handler): 4 fixed conductivity sensing tips. 1-500 µl
		+	* MCA (Multichannel Arm): 96 Tip head uses disposable tips (50 or 200 µl)
		+
		+	===CARRIERS===
		+	Johnny 5's deck is configured with the following carriers.
		+	* 1 fixed tip washer.
		+	* 3 15ml tube carriers.
		+	* 2 temperature controlled carriers (holds 6 plates)
		+	* 3 non-temperature controlled carriers (hold 10 plates)
		+	* 3 spots for tops.
		+
		+	[[BioMicroCenter:Tecan_CAL\| ''' SCHEDULE TIME ON JOHNNY 5 ''']]
		+	'' Note, time must be booked in 2 hour increments. ''
		+
		+	== B9 ==
		+
		+	== EVOWARE ==
		+
		+	== LABWARE ==

-	~~==TECAN FREEDOM EVO 150==~~

-	~~ All users must be trained before being allowed to use the Evo ~~

	[[Image:Tn_PR004749.jpg\|right\|97px]]		[[Image:Tn_PR004749.jpg\|right\|97px]]
-	The BioMicro Center currently hosts two Tecan Freedom EVO 150s. The EVO is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting. The Freedom EVO is user-friendly and runs the EVOware software package. The EVOware package allows for the creation of a virtual image of the platform and allows the scientist to easily implement methods with a drag-and-drop graphical user interface.	+

	The second EVO150 arrived in the BioMicro Center in June 2010. This robot has several additional features focused on screening 96 and 384 well plates. These include a 96-channel pipette arm, a remote manipulator arm, plate and tip stackers, plate shakers and incubators, etc. For more information about the Tecans, please email [[BioMicroCenter:People\|Stuart Levine]]		The second EVO150 arrived in the BioMicro Center in June 2010. This robot has several additional features focused on screening 96 and 384 well plates. These include a 96-channel pipette arm, a remote manipulator arm, plate and tip stackers, plate shakers and incubators, etc. For more information about the Tecans, please email [[BioMicroCenter:People\|Stuart Levine]]
Line 41:		Line 69:
	=== SCHEDULING ===		=== SCHEDULING ===
	Access to the Tecan liquid handling robots is available in 2 hour blocks. You can schedule use of either robot by clicking on the link below.		Access to the Tecan liquid handling robots is available in 2 hour blocks. You can schedule use of either robot by clicking on the link below.
-	* [[BioMicroCenter:Tecan_Freedom_Evo\|Tecan Evo]] - ~~[[BioMicroCenter:Tecan_CAL\|Johnny 5 (LiHa/MCA)]]~~ - [[BioMicroCenter:Tecan_CAL2\|B9 (RoMa/MCA)]]	+	* [[BioMicroCenter:Tecan_Freedom_Evo\|Tecan Evo]] - - [[BioMicroCenter:Tecan_CAL2\|B9 (RoMa/MCA)]]

	=== [[BioMicroCenter:Pricing\|COST]] ===		=== [[BioMicroCenter:Pricing\|COST]] ===
	In order to defray the costs of the annual service contract, each 2 hour session on the EVO will be charged '''$50.'''. Tips and 96 well plates are available for purchase from the BioMicro center or obtained from your own lab. Additionally, training is required for all new users. A training session is $100		In order to defray the costs of the annual service contract, each 2 hour session on the EVO will be charged '''$50.'''. Tips and 96 well plates are available for purchase from the BioMicro center or obtained from your own lab. Additionally, training is required for all new users. A training session is $100

Barbara Karampalas: /* TECAN FREEDOM EVO 150 */

2011-09-26T15:38:22Z

TECAN FREEDOM EVO 150

←Older revision		Revision as of 15:38, 26 September 2011
Line 8:		Line 8:
	The BioMicro Center currently hosts two Tecan Freedom EVO 150s. The EVO is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting. The Freedom EVO is user-friendly and runs the EVOware software package. The EVOware package allows for the creation of a virtual image of the platform and allows the scientist to easily implement methods with a drag-and-drop graphical user interface.		The BioMicro Center currently hosts two Tecan Freedom EVO 150s. The EVO is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting. The Freedom EVO is user-friendly and runs the EVOware software package. The EVOware package allows for the creation of a virtual image of the platform and allows the scientist to easily implement methods with a drag-and-drop graphical user interface.

-	The second EVO150 arrived in the BioMicro Center in June 2010. This robot has several additional features focused on screening 96 and 384 well plates. These include a 96-channel pipette arm, a remote manipulator arm, plate and tip stackers, plate shakers and incubators, etc. For more information about the Tecans, please email[[BioMicroCenter:People\|Stuart Levine]]	+	The second EVO150 arrived in the BioMicro Center in June 2010. This robot has several additional features focused on screening 96 and 384 well plates. These include a 96-channel pipette arm, a remote manipulator arm, plate and tip stackers, plate shakers and incubators, etc. For more information about the Tecans, please email [[BioMicroCenter:People\|Stuart Levine]]

	[[Image:BioMicroCenter_Tecan2_Sim.png\|frame\|none\|200px\|left\|alt=TecanImage\|Simulated view of new Tecan EVO150]]		[[Image:BioMicroCenter_Tecan2_Sim.png\|frame\|none\|200px\|left\|alt=TecanImage\|Simulated view of new Tecan EVO150]]