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{{BioMicroCenter}}
{{BioMicroCenter}}
  ** All users must be trained before being allowed to use the Tecans **


The BioMicro Center currently hosts two Tecan Freedom EVO 150s named Johnny5 and B9. Johnny5 is optimized for cherry picking applications while B9 is optimized for screening. The Tecan's in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+  a new High-Throughput core is being developed by the [http://ki.mit.edu/sbc Koch Institute.]  
''' ** All users must be trained before being allowed to use the equipment **'''<BR><BR>
The BioMicro Center currently hosts three automated liquid handlers: two [https://lifesciences.tecan.com/products/liquid_handling_and_automation/freedom_evo_series Tecan Freedom EVO 150s] and one [https://www.ttplabtech.com/products/liquid-handling/mosquito-hv/ SPT Labtech Mosquito HV]. One EVO is optimized for cherry picking applications while the other is optimized for sample processing and assay set-up. The Tecans and Mosquito in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+  a new High-Throughput core has been developed by the [http://ki.mit.edu/sbc Koch Institute.] <BR><BR>


==JOHNNY 5==
The Tecans are offered as a walk up service or as an assisted service. Due to the steep learning curves on the robots, we strongly encourage users to allow BMC staff to assist them in automating protocols and programming the instruments and then simply execute the programs as a walk up. Machine downtime is extremely limited and there is minimal time for practice runs.
[[Image:BMC_Johnny5_robot.jpg|right|100px]]
"Johnny5" is the BioMicro Center robot optimized for cherry picking. It is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96).  The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting.  


===ARMS===
== JOHNNY 5 ==
[[Image:BMC_Johnny5.jpg|right|300px]]
{|
Johnny 5 has the following arms:
|- style="vertical-align: top;"
* LiHa (Liquid handler): 4 fixed conductivity sensing tips. 1-500 µl
| style="width:350px;" |
* MCA (Multichannel Arm): 96 Tip head uses disposable tips (50 or 200 µl)
  {| class="wikitable" border=1
 
  |-
===CARRIERS===
  ! style="text-align: center; width: 150px;" | Instrument
Johnny 5's deck is configured with the following carriers.
  ! style="text-align: center; width: 300px;" | Tecan EVO 150
* 1 fixed tip washer.
  |-
* 3 15ml tube carriers.
  | style="text-align: center; height: 2em;" | USAGE
* 2 temperature controlled SBS carriers (holds 6 plates)
  | style="text-align: center; height: 2em;" | Walkup - MIT only <br> Approval Required
* 3 non-temperature controlled SBS carriers (hold 10 plates)
  |-
* 3 spots for tips.
  | style="text-align: center; height: 2em;" | UNIT
  | style="text-align: center; height: 2em;" | Per hour + Consumables
  |-
  | style="text-align: center; height: 2em;" | ARMS
  | style="text-align: left; height: 2em;" |
* LiHa span4 (2&micro;L-200&micro;L)
* MCA96 (50&micro;L/200&micro;L tips)
  |-
  | style="text-align: center; height: 2em;" | CARRIERS
  | style="text-align: left; height: 2em;" |
* 1 fixed tip washer  
* 6 15ml tube carriers  
* 3 temperature controlled SBS carriers  
* 24 non-temperature controlled SBS carriers  
* 4 racks for 1.5ml tubes (24 each)
* 4 racks for 1.5ml tubes (24 each)
* UV light for decontamination
  |-
  | style="text-align: center; height: 2em;" | SCHEDULE USAGE
  | style="text-align: center; height: 2em;" | [https://mit.ilabsolutions.com/equipment/show/262347/?tab=schedule ilabs]
  |-
  | style="text-align: center; height: 2em;" | NEW USERS
  | style="text-align: center; height: 2em;" | New users should request training by emailing the [mailto:biomicro@mit.edu  BioMicro Center team].
  |-
  | style="text-align: center; height: 2em;" | DONATED BY
  | style="text-align: center; height: 2em;" | Department of Biology
  |}
|
<br>
:"Johnny5" or "J5" is the BioMicro Center robot optimized for cherry picking. It is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96).  The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing.  The EVO can accurately handle volumes between 2 and 200 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting. <BR><BR>


[[BioMicroCenter:Tecan_CAL| ''' SCHEDULE TIME ON JOHNNY 5 ''']]
:J5 is used by the BioMicro Center primarily for plate normalization as part of high-throughput library production. Users should plan experiments in advance to coordinate usage with BMC staff.<BR>
'' Note, time must be booked in 2 hour increments. ''
<br>
:::::::[[IMAGE:BMC_Johnny5.jpg | inline | 300px]] &nbsp;&nbsp;&nbsp;&nbsp; [[IMAGE:BMC_Johnny5_robot.jpg | inline | 110px]]
|
|}


== B9 ==
== B9 ==
[[Image:BMC_B9_robot.png|right|100px]]
{|
"B9" is the BioMicro Center EVO150 optimized for high throughput screening. B9 has a 96 tip head for rapid pipetting and a robotic arm for moving plates around the deck. The robot has 4 plate stackers which can be used to add or remove plates during a run. B9 was donated to the BioMicro Center by [http://gilbertlab.mit.edu/gilbertlabpeople.html Dr. Wendy Gilbert] through a small instrumentation grant from the NIH.
|- style="vertical-align: top;"
 
| style="width:350px;" |
===ARMS===
  {| class="wikitable" border=1
[[Image:BMC_B9.png|right|300px]]
  |-
B9 has the following arms:
  ! style="text-align: center; width: 150px;" | Instrument
* MCA (Multichannel Arm): 96 Tip head uses disposable tips (50 or 200 µl)
  ! style="text-align: center; width: 325px;" | Tecan EVO 150
* RoMa (Remote Manipulator): Robotic are to move plates around.
  |-
 
  | style="text-align: center; height: 2em;" | USAGE
===CARRIERS===
  | style="text-align: center; height: 2em;" | Walkup - MIT only <br> Approval Required
B9 has the following Carriers:
  |-
* 1 rack for 3 non-SBS tip racks
  | style="text-align: center; height: 2em;" | UNIT
  | style="text-align: center; height: 2em;" | Per hour + Consumables
  |-
  | style="text-align: center; height: 2em;" | ARMS
  | style="text-align: left; height: 2em;" |
* MCA96 (50&micro;L/200&micro;L tips)
* RoMa (Robotic Manipulator)
  |-
  | style="text-align: center; height: 2em;" | CARRIERS
  | style="text-align: left; height: 2em;" |
* 1 rack for 2 stacks of nested tips with chute
* 1 rack for 2 stacks of nested tips with chute
* 3 racks for SBS tips (12 spots total)
* 3 temperature controlled SBS format positions on one carrier.
* [http://www.tecan.com/platform/apps/product/index.asp?MenuID=3416&ID=7010&Menu=1&Item=21.1.9.26 TeVac vacuum manifold]
* 16 SBS format positions on 4 carrier
<!-- * [http://www.tecan.com/platform/apps/product/index.asp?MenuID=3416&ID=7010&Menu=1&Item=21.1.9.26 TeVac vacuum manifold] -->
* [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1399&ID=973&Menu=1&Item=21.1.9.11 Vortexer (Te-Shake)]
* [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1399&ID=973&Menu=1&Item=21.1.9.11 Vortexer (Te-Shake)]
* 2 on deck racks for short plates
* 4 on deck racks for short plates
* Shaking Incubator
* Shaking Incubator
* [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1214&ID=611&Menu=1&Item=21.1.9.13 4 Stackers (TeStack)]
* [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1214&ID=611&Menu=1&Item=21.1.9.13 4 Stackers (TeStack)]
* Alpaqua 96-well Magnets
* 384-well Magnets
  |-
  | style="text-align: center; height: 2em;" | SCHEDULE USAGE
  | style="text-align: center; height: 2em;" | [https://mit.ilabsolutions.com/equipment/show/262347/?tab=schedule ilabs]
  |-
  | style="text-align: center; height: 2em;" | NEW USERS
  | style="text-align: center; height: 2em;" | New users should request training by emailing the [mailto:biomicro@mit.edu  BioMicro Center team].
  |-
  | style="text-align: center; height: 2em;" | DONATED BY
  | style="text-align: center; height: 2em;" | Dr. WENDY GILBERT (now at Yale)
  |}
|
<br>
:"B9" is the BioMicro Center EVO150 optimized for nucleic acid cleans and quantification assays. B9 has a 96-channel pipette arm (MCA96) and a robotic manipulator (RoMa) arm for moving plates around the deck. This instrument supports a broad range of fluid transfer operations including reagent addition, sample transfer, plate-to-plate transfer, serial dilutions, and within-well mixing. This robot contains a waste chute and 4 plate stackers which combined with the RoMa arm can be used to add or remove plates makes B9 a highly dynamic automation platform. B9 was donated to the BioMicro Center by Dr. Wendy Gilbert through a shared instrumentation grant from the NIH. <BR><BR>


[[BioMicroCenter:Tecan_CAL2|'''Schedule time on B9''']]
:B9 is regularly used by the BioMicro Center for nucleic acid cleans, SYBR and qPCR assay setup, and Illumina Library preparation. <br>
'' Note, time must be booked in 2 hour increments. ''
<br>
:::::::[[IMAGE:BMC_B9.png | inline | 300px]] &nbsp;&nbsp; [[Image:BMC_B9_robot.png | inline | 125px]]
|
|}


== EVOWARE ==
== SPT LABTECH MOSQUITO HV ==
{|
{|
|[[Image:BMC_Evoware.png|300px]]
|- style="vertical-align: top;"
| The Tecan EVO currently runs using the [http://www.tecan.com/platform/apps/product/index.asp?MenuID=1458&ID=1084&Menu=1&Item=21.7.1 EVOware] software package. EVOware can be run in either of two modes: directly connected to the Tecan EVO to allow control of the robot, or on your desktop computer in a simulation mode. Simulation mode is extremely useful in that it allows the user to pre-design the steps in their program. All users must download EVOware and design their protocols to be brought to the EVO.
| style="width:350px;" |
  {| class="wikitable" border=1
  |-
  ! style="text-align: center; width: 150px;" | Instrument
  ! style="text-align: center; width: 325px;" | Mosquito HV
  |-
  | style="text-align: center; height: 2em;" | USAGE
  | style="text-align: center; height: 2em;" | Not available as a walkup service
  |-
  | style="text-align: center; height: 2em;" | UNIT
  | style="text-align: center; height: 2em;" | Included in high-throughput library preparation
  |-
  | style="text-align: center; height: 2em;" | ARMS
  | style="text-align: center; height: 2em;" | 8-tip disposable (0.2&micro;L - 5&micro;L)
  |-
  | style="text-align: center; height: 2em;" | CARRIERS
  | style="text-align: left; height: 2em;" |
* 5 SBS positions
* Humidifying chamber
  |-
  | style="text-align: center; height: 2em;" | SCHEDULE USAGE
  | style="text-align: center; height: 2em;" | Contact [[BioMicroCenter:People|Tatum Murdock]] for special requests
  |-
  | style="text-align: center; height: 2em;" | DONATED BY
  | style="text-align: center; height: 2em;" | SPT Labtech (on loan)
  |}
|
<br>
<br>
* [[BioMicroCenter:EVOware | Obtain a copy of EVOware]]
:The SPT Labtech Mosquito HV is a critical instrument in the BioMicro Center's goal of automated high-throughput library production. The accurate low volume pipetting offered by this liquid handler has allowed for miniaturization of a number of protocols. While the instrument does not provide a 'walk away' level of automation, programming the Mosquito is significantly easier than the Tecans and prototyping methods is much more rapid. <BR><BR>
:The Mosquito is used in all HTL protocols in the BioMicro Center.
|
<br>
[[Image:BMC_MOSQUITO.png | 300px]]
|}
|}


== LABWARE ==
== verrity Chemagic 360 ==
Labware refers to the plastic consumables used by the robot. These include the tips, tubes, plates, etc. Each piece of labware must be individually prepared for the EVO and each program must refer to specific pieces of labware. Failure to properly set up labware can cause expensive damage to the machine and significant down time. The BioMicro Center stocks several types of labware which are available to users:
{|
 
|- style="vertical-align: top;"
=== TIPS (MCA) ===
| style="width:350px;" |
* 50ul standard
  {| class="wikitable" border=1
* 50ul filter,low bind, sterile
  |-
* 50ul nested stack
  ! style="text-align: center; width: 150px;" | Instrument
* 200ul standard
  ! style="text-align: center; width: 325px;" | Chemagic360
* 200ul filter, low bind, sterile
  |-
 
  | style="text-align: center; height: 2em;" | USAGE
=== PLATES ===
  | style="text-align: center; height: 2em;" | Available as a walkup service
* 96well Axygen full skirted PCR plate (PCR-96-FS-C)
  |-
* 96well Axygen RT-PCR plates
  | style="text-align: center; height: 2em;" | UNIT
* 384well Roche RT-PCR plates
  | style="text-align: center; height: 2em;" | Included in high-throughput sample preparation
 
  |-
== SERVICES ==
  | style="text-align: center; height: 2em;" | chemagic dispenser
 
  | style="text-align: center; height: 2em;" | 12-tip 3-row array
Use of the BioMicro Center Tecans can either be supervised or full-service.
  |-
 
  | style="text-align: center; height: 2em;" | Rod Head
=== Supervised ===
  | style="text-align: left; height: 2em;" | 96 well EM head
In supervised use, the BioMicro Center will provide training for a new user. The user is then approved to work on the Tecans on their own. Newer users should work when BioMicro Center staff are available to answer questions. Usage of the robots for practice is *not* charged but has lowest priority. Practice time must be booked on the calender and indicated as practice. Practice time must also be logged on the time sheet next to the robot. A valid cost object number must be on record in the BioMicro Center and consumables are still charged. Once the user is using real samples, time is charged at our current rate found on the [[BioMicroCenter:Pricing|pricing page]].
  |-
 
  | style="text-align: center; height: 2em;" | SCHEDULE USAGE BY PURIFICATION TYPE
'''FAILURE TO FOLLOW BMC POLICIES ON SAFETY OR USAGE OF THE ROBOTS *WILL* RESULT IN REMOVAL OF YOUR ACCESS TO THE BIOMICRO CENTER'''
  | style="text-align: center; height: 2em;" | Contact [[BioMicroCenter:People|Avanyish Toniappa]] a month ahead
 
  |-
=== Full Service ===
  | style="text-align: center; height: 2em;" | DONATED BY
In Full Service use, the BioMicro Center will be responsible for creating scripts, labware, and liquid classes to handle a user's protocol. Once completed, the user will be trained to run the protocol. Setup time will be estimated at a preliminary meeting and a cost and timeline will be estimated for the project. Once the protocol is set up, standard usage rates apply. Rates are on the [[BioMicroCenter:Pricing|pricing page]].
  | style="text-align: center; height: 2em;" |
* MIT Stem Cell Initiative
* Dr Jacqueline A. Lees
  |}
|
<br>
:The verrity (formerly Perkin-Elmer) Chemagic360 is a critical instrument in the BioMicro Center's goal of automated high-throughput library production. 96 tissue or cell lysates take up to 2 hours for a thorough bead based clean. This instrument provides 'walk away' automation, but does require setup steps such as creating lysates before loading, and unloading the instrument properly. There are no optional programming options. <BR><BR>
:If a kit for a specific purification needs to be ordered, it will take a month to arrive.
|
<br>
[[Image:Chemagic360.jpg | 300px]]
|}

Latest revision as of 12:12, 18 April 2024

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

** All users must be trained before being allowed to use the equipment **

The BioMicro Center currently hosts three automated liquid handlers: two Tecan Freedom EVO 150s and one SPT Labtech Mosquito HV. One EVO is optimized for cherry picking applications while the other is optimized for sample processing and assay set-up. The Tecans and Mosquito in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+ a new High-Throughput core has been developed by the Koch Institute.

The Tecans are offered as a walk up service or as an assisted service. Due to the steep learning curves on the robots, we strongly encourage users to allow BMC staff to assist them in automating protocols and programming the instruments and then simply execute the programs as a walk up. Machine downtime is extremely limited and there is minimal time for practice runs.

JOHNNY 5

Instrument Tecan EVO 150
USAGE Walkup - MIT only
Approval Required
UNIT Per hour + Consumables
ARMS
  • LiHa span4 (2µL-200µL)
  • MCA96 (50µL/200µL tips)
CARRIERS
  • 1 fixed tip washer
  • 6 15ml tube carriers
  • 3 temperature controlled SBS carriers
  • 24 non-temperature controlled SBS carriers
  • 4 racks for 1.5ml tubes (24 each)
  • UV light for decontamination
SCHEDULE USAGE ilabs
NEW USERS New users should request training by emailing the BioMicro Center team.
DONATED BY Department of Biology


"Johnny5" or "J5" is the BioMicro Center robot optimized for cherry picking. It is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 2 and 200 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting.

J5 is used by the BioMicro Center primarily for plate normalization as part of high-throughput library production. Users should plan experiments in advance to coordinate usage with BMC staff.


inline      inline

B9

Instrument Tecan EVO 150
USAGE Walkup - MIT only
Approval Required
UNIT Per hour + Consumables
ARMS
  • MCA96 (50µL/200µL tips)
  • RoMa (Robotic Manipulator)
CARRIERS
  • 1 rack for 2 stacks of nested tips with chute
  • 3 temperature controlled SBS format positions on one carrier.
  • 16 SBS format positions on 4 carrier
  • Vortexer (Te-Shake)
  • 4 on deck racks for short plates
  • Shaking Incubator
  • 4 Stackers (TeStack)
  • Alpaqua 96-well Magnets
  • 384-well Magnets
SCHEDULE USAGE ilabs
NEW USERS New users should request training by emailing the BioMicro Center team.
DONATED BY Dr. WENDY GILBERT (now at Yale)


"B9" is the BioMicro Center EVO150 optimized for nucleic acid cleans and quantification assays. B9 has a 96-channel pipette arm (MCA96) and a robotic manipulator (RoMa) arm for moving plates around the deck. This instrument supports a broad range of fluid transfer operations including reagent addition, sample transfer, plate-to-plate transfer, serial dilutions, and within-well mixing. This robot contains a waste chute and 4 plate stackers which combined with the RoMa arm can be used to add or remove plates makes B9 a highly dynamic automation platform. B9 was donated to the BioMicro Center by Dr. Wendy Gilbert through a shared instrumentation grant from the NIH.

B9 is regularly used by the BioMicro Center for nucleic acid cleans, SYBR and qPCR assay setup, and Illumina Library preparation.


inline    inline

SPT LABTECH MOSQUITO HV

Instrument Mosquito HV
USAGE Not available as a walkup service
UNIT Included in high-throughput library preparation
ARMS 8-tip disposable (0.2µL - 5µL)
CARRIERS
  • 5 SBS positions
  • Humidifying chamber
SCHEDULE USAGE Contact Tatum Murdock for special requests
DONATED BY SPT Labtech (on loan)


The SPT Labtech Mosquito HV is a critical instrument in the BioMicro Center's goal of automated high-throughput library production. The accurate low volume pipetting offered by this liquid handler has allowed for miniaturization of a number of protocols. While the instrument does not provide a 'walk away' level of automation, programming the Mosquito is significantly easier than the Tecans and prototyping methods is much more rapid.

The Mosquito is used in all HTL protocols in the BioMicro Center.


verrity Chemagic 360

Instrument Chemagic360
USAGE Available as a walkup service
UNIT Included in high-throughput sample preparation
chemagic dispenser 12-tip 3-row array
Rod Head 96 well EM head
SCHEDULE USAGE BY PURIFICATION TYPE Contact Avanyish Toniappa a month ahead
DONATED BY
  • MIT Stem Cell Initiative
  • Dr Jacqueline A. Lees


The verrity (formerly Perkin-Elmer) Chemagic360 is a critical instrument in the BioMicro Center's goal of automated high-throughput library production. 96 tissue or cell lysates take up to 2 hours for a thorough bead based clean. This instrument provides 'walk away' automation, but does require setup steps such as creating lysates before loading, and unloading the instrument properly. There are no optional programming options.

If a kit for a specific purification needs to be ordered, it will take a month to arrive.


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