BioMicroCenter:Sequencing Quality Control: Difference between revisions
(New page: {{BioMicroCenter}} == Quality Control for Samples Submitted for Illumina Sequqncing == When submitting samples to the BMC for Illumina sequencing it is very important to supply us with ...) |
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Revision as of 09:10, 8 January 2009
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Quality Control for Samples Submitted for Illumina Sequqncing
When submitting samples to the BMC for Illumina sequencing it is very important to supply us with a reliable measurement of the concentration and quantity of starting sample. Having this information allows us to insure reliability of cluster density to maximize the number of reads per lane and therefore maximize the quality of data produced. QC can either be run before sample submission and specified upon testing request or can be run by the BMC. We strongly recommend that you allow us to run QC of samples.
Below are some of the most common techniques used for QCing samples for sequencing:
- Bioanalyzer - More reliable with samples of higher concentration. Available at the BMC for $10 per sample
- Nanodrop - not recommended for sequencing samples because this method is not reliable enough at such low concentrations
- Qubit - not tested at the BMC but information about this application can be found at [1]
- RT-PCR, SYBERgreen assay - coming soon to the BMC!!
