BioMicroCenter:Sequencing - Revision history

Michael Gravina: /* Genome Analyzer IIx */

2013-04-09T12:46:36Z

Genome Analyzer IIx

←Older revision		Revision as of 12:46, 9 April 2013
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-	\|colspan="3" align=center *''Second number pertains to reads greater than 40 nt.''	+	\|colspan="3" align=center \|*''Second number pertains to reads greater than 40 nt.''
	\|}		\|}

Michael Gravina: /* Genome Analyzer IIx */

2013-04-09T12:45:35Z

Genome Analyzer IIx

←Older revision		Revision as of 12:45, 9 April 2013
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-	\|	+	\|colspan="3" align=center *''Second number pertains to reads greater than 40 nt.''
-	\|	+
-	\|*''Second number~~<br>~~pertains to~~<br>~~reads greater~~<br>~~than 40 nt.''	+
	\|}		\|}

Michael Gravina: /* Genome Analyzer IIx */

2013-04-08T17:59:01Z

Genome Analyzer IIx

←Older revision		Revision as of 17:59, 8 April 2013
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-	Using fewer lanes on each flowcell has allowed us to decrease the cycle time by not imaging all the lanes. In a typical 8 lane run, 20 minutes is spent doing chemistry followed by 40 minutes of imaging (each lane takes ~5 minutes to image). Therefore, a 2 lane flowcell runs twice as fast as an 8 lane flowcell. Also, since the chemistry is not running in to all of the lanes, each sequencing kit can go to a longer read length. The relationships are summarized in the chart on the left. Pricing is set on the number of lanes you are using, the number of days you are running the GAII, and the number of sequencing kits you are using. For example, if you wanted to run a 96+96 PE flowcell using 2 lanes, the cost would be the initial cost for the 2 lane PE flowcell plus an additional 3 days (one day is included in the original price) plus ~~a second~~ sequencing ~~kit~~. ~~That~~ kit would not be completely used up (you would have an extra ~~48nt~~ left that would be thrown away).<BR><BR>	+	Using fewer lanes on each flowcell has allowed us to decrease the cycle time by not imaging all the lanes. In a typical 8 lane run, 20 minutes is spent doing chemistry followed by 40 minutes of imaging (each lane takes ~5 minutes to image). Therefore, a 2 lane flowcell runs twice as fast as an 8 lane flowcell. Also, since the chemistry is not running in to all of the lanes, each sequencing kit can go to a longer read length. The relationships are summarized in the chart on the left. Pricing is set on the number of lanes you are using, the number of days you are running the GAII, and the number of sequencing kits you are using. For example, if you wanted to run a 75+75 PE flowcell using 2 lanes, the cost would be the initial cost for the 2 lane PE flowcell plus an additional 3 days (one day is included in the original price) plus two additional sequencing kits. The last kit would not be completely used up (you would have an extra 18nt left that would be thrown away).<BR><BR>

	The GAII/GAIIx is ideal for:		The GAII/GAIIx is ideal for:

Michael Gravina: /* Genome Analyzer IIx */

2013-04-08T17:56:00Z

Genome Analyzer IIx

←Older revision		Revision as of 17:56, 8 April 2013
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	\| 140/81*		\| 140/81*
	\|-		\|-
-	\|*''Second number<br>pertains to ~~reads~~<br>greater than 40 nt.''	+	\|
		+	\|
		+	\|*''Second number<br>pertains to<br>reads greater<br>than 40 nt.''
	\|}		\|}

Michael Gravina: /* Genome Analyzer IIx */

2013-04-08T17:55:18Z

Genome Analyzer IIx

←Older revision		Revision as of 17:55, 8 April 2013
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	\| 140/81*		\| 140/81*
	\|-		\|-
-	\|*''Second number	+	\|*''Second number<br>pertains to reads<br>greater than 40 nt.''
-	\|pertains to reads	+
-	\|greater than 40 nt.''	+
	\|}		\|}

Michael Gravina: /* Genome Analyzer IIx */

2013-04-08T17:54:09Z

Genome Analyzer IIx

←Older revision		Revision as of 17:54, 8 April 2013
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	\| 72		\| 72
	\| 140/81*		\| 140/81*
-	\|~~}<br><br><br><br><br><br><br><br>~~	+	\|-
-	~~{\| border=0 align="right"~~	+	\|*''Second number
-	\|*''Second number pertains to reads greater than 40 nt.''	+	\|pertains to reads
		+	\|greater than 40 nt.''
	\|}		\|}

Michael Gravina: /* Genome Analyzer IIx */

2013-04-08T17:53:23Z

Genome Analyzer IIx

←Older revision		Revision as of 17:53, 8 April 2013
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	\| 140/81*		\| 140/81*
-	\|}<br>	+	\|}<br><br><br><br><br><br><br><br>
	{\| border=0 align="right"		{\| border=0 align="right"
	\|*''Second number pertains to reads greater than 40 nt.''		\|*''Second number pertains to reads greater than 40 nt.''

Michael Gravina: /* Genome Analyzer IIx */

2013-04-08T17:53:01Z

Genome Analyzer IIx

←Older revision		Revision as of 17:53, 8 April 2013
Line 59:		Line 59:
	\| 72		\| 72
	\| 140/81*		\| 140/81*
-	\|}	+	\|}<br>
	{\| border=0 align="right"		{\| border=0 align="right"
	\|*''Second number pertains to reads greater than 40 nt.''		\|*''Second number pertains to reads greater than 40 nt.''

Michael Gravina: /* Genome Analyzer IIx */

2013-04-08T17:52:42Z

Genome Analyzer IIx

←Older revision		Revision as of 17:52, 8 April 2013
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	\| 72		\| 72
	\| 140/81*		\| 140/81*
-	\|-	+	\|}
		+	{\| border=0 align="right"
	\|*''Second number pertains to reads greater than 40 nt.''		\|*''Second number pertains to reads greater than 40 nt.''
	\|}		\|}

Michael Gravina: /* Genome Analyzer IIx */

2013-04-08T17:51:29Z

Genome Analyzer IIx

←Older revision		Revision as of 17:51, 8 April 2013
Line 59:		Line 59:
	\| 72		\| 72
	\| 140/81*		\| 140/81*
		+	\|-
		+	\|*''Second number pertains to reads greater than 40 nt.''
	\|}		\|}
-	~~&#42: ''Second number pertains to reads greater than 40 nt.''<br><br>~~

	Using fewer lanes on each flowcell has allowed us to decrease the cycle time by not imaging all the lanes. In a typical 8 lane run, 20 minutes is spent doing chemistry followed by 40 minutes of imaging (each lane takes ~5 minutes to image). Therefore, a 2 lane flowcell runs twice as fast as an 8 lane flowcell. Also, since the chemistry is not running in to all of the lanes, each sequencing kit can go to a longer read length. The relationships are summarized in the chart on the left. Pricing is set on the number of lanes you are using, the number of days you are running the GAII, and the number of sequencing kits you are using. For example, if you wanted to run a 96+96 PE flowcell using 2 lanes, the cost would be the initial cost for the 2 lane PE flowcell plus an additional 3 days (one day is included in the original price) plus a second sequencing kit. That kit would not be completely used up (you would have an extra 48nt left that would be thrown away).<BR><BR>		Using fewer lanes on each flowcell has allowed us to decrease the cycle time by not imaging all the lanes. In a typical 8 lane run, 20 minutes is spent doing chemistry followed by 40 minutes of imaging (each lane takes ~5 minutes to image). Therefore, a 2 lane flowcell runs twice as fast as an 8 lane flowcell. Also, since the chemistry is not running in to all of the lanes, each sequencing kit can go to a longer read length. The relationships are summarized in the chart on the left. Pricing is set on the number of lanes you are using, the number of days you are running the GAII, and the number of sequencing kits you are using. For example, if you wanted to run a 96+96 PE flowcell using 2 lanes, the cost would be the initial cost for the 2 lane PE flowcell plus an additional 3 days (one day is included in the original price) plus a second sequencing kit. That kit would not be completely used up (you would have an extra 48nt left that would be thrown away).<BR><BR>