BioMicroCenter:SPRI-Works
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The BMC is currently testing the Beckman SPRIworks library preparation system. This platform is a fully automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. With the exception of Enrichment, it performs all of the traditional Illumina protocol steps listed on the Illumina Library Preparation page. A total of 10 libraries may be simultaneously prepared in 5 hours for Illumina sequencing.
How it Works
The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all of the reagents required to prepare a single library.
This platform utilizes AMPureXP DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel-based size selection. There are two size range options available, 100-400bp or 300-600bp. There is also the choice to perform no size selection. After the size selection and adapter ligation process is complete on the SPRI-TE, samples will be ready for PCR Enrichment and clean-up to complete the library prep process.
For more information on the SPRI-Works system please visit www.spriworks.com
Sample Submission
All samples submitted for direct SPRI-Works library preparation must be submitted with user supplied adapter mix. Please note that samples will need to be resubmitted separately for Illumina sequencing.