BioMicroCenter:SPRI-Works: Difference between revisions
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The SPRI-Works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that contains the liquid handling program, and a cartridge containing all of the reagents required to prepare a single library. | The SPRI-Works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that contains the liquid handling program, and a cartridge containing all of the reagents required to prepare a single library. | ||
The platform utilizes AMPureXP DNA-binding magnetic beads to purify fragmented DNA and perform size selection, eliminating the need for column purification and gel-based size selection. There are three size range options available, 200-400bp, 300-600bp, or no size selection.<br> | The platform utilizes AMPureXP DNA-binding magnetic beads to purify fragmented DNA and perform size selection, eliminating the need for column purification and gel-based size selection. There are three size range options available, 200-400bp, 300-600bp, or no size selection. (These ranges apply to the inserts together with the partial adapters. For optimal yield, libraries should be fragmented so that average fragment size is approximately 60nt shorter than the chosen size range, to allow for the partial adapters.)<br> | ||
After the adapter ligation and size selection steps are complete, samples are ready for PCR enrichment and clean-up to complete the library prep process. | After the adapter ligation and size selection steps are complete, samples are ready for PCR enrichment and clean-up to complete the library prep process. | ||
Revision as of 06:59, 29 September 2011
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The SPRI-te Nucleic Acid Extractor is a fully-automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. It performs all of the traditional Illumina protocol steps listed on the Illumina Library Preparation page with the exception of PCR enrichment. A total of 10 libraries may be prepared simultaneously in 5 hours.
How it Works
The SPRI-Works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that contains the liquid handling program, and a cartridge containing all of the reagents required to prepare a single library.
The platform utilizes AMPureXP DNA-binding magnetic beads to purify fragmented DNA and perform size selection, eliminating the need for column purification and gel-based size selection. There are three size range options available, 200-400bp, 300-600bp, or no size selection. (These ranges apply to the inserts together with the partial adapters. For optimal yield, libraries should be fragmented so that average fragment size is approximately 60nt shorter than the chosen size range, to allow for the partial adapters.)
After the adapter ligation and size selection steps are complete, samples are ready for PCR enrichment and clean-up to complete the library prep process.
For more information on the SPRI-Works system please visit www.spriworks.com.
Sample Submission
Input sample concentrations on the SPRI-te are variable (we have successfully run samples as low as 0.3ng/uL). We suggest that you submit as much volume as possible since the first step of the protocol brings all sample volumes up to 100uL in water.
All samples submitted for BMC Fragmented DNA sample prep (SPRI-only) must be submitted with user supplied adapter mix. Adapter mixes should be pre-diluted and should have enough volume for 10uL of adapter per sample. For recommended dilutions for various sample concentrations, please see the chart below:
Please note that SPRI-only samples will need to be re-submitted separately if you wish to sequence with us later on.
