BioMicroCenter:SPRI-Works: Difference between revisions
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[[Image: BioMicro_SPRIworks.jpg|right|200px]] | [[Image: BioMicro_SPRIworks.jpg|right|200px]] | ||
The BMC is currently testing the Beckman SPRIworks library preparation system. This platform is a fully automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. | The BMC is currently testing the Beckman SPRIworks library preparation system. This platform is a fully automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. With the exception of Enrichment, it performs all of the traditional Illumina protocol steps listed on the [[BioMicroCenter:Illumina Library Preparation|Illumina Library Preparation page]]. A total of 10 libraries may be simultaneously prepared in 5 hours for Illumina sequencing. | ||
==How it Works== | ==How it Works== | ||
[[Image:spri-works.jpg|right|200px]] | [[Image:spri-works.jpg|right|200px]] | ||
The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all the reagents required to prepare a single library. | The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all of the reagents required to prepare a single library. | ||
This platform utilizes AMPureXP DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel-based size selection. There are two size range options available, 100-400bp or 300-600bp. There is also the choice to perform no size selection. | This platform utilizes AMPureXP DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel-based size selection. There are two size range options available, 100-400bp or 300-600bp. There is also the choice to perform no size selection. After the size selection and adapter ligation process is complete on the SPRI-TE, samples will be ready for PCR Enrichment and clean-up to complete the library prep process. | ||
For more information on the SPRI-Works system please visit www.spriworks.com | For more information on the SPRI-Works system please visit www.spriworks.com | ||
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==Sample Submission== | ==Sample Submission== | ||
All samples submitted for direct SPRI-Works library preparation must be submitted with '''user supplied''' adapter mix. Please note that samples will need to be | All samples submitted for direct SPRI-Works library preparation must be submitted with '''user supplied''' adapter mix. Please note that samples will need to be resubmitted separately for Illumina sequencing. | ||
[[BioMicroCenter:Forms#Downloads|Sample Submission Forms]] | [[BioMicroCenter:Forms#Downloads|Sample Submission Forms]] | ||
Revision as of 08:19, 4 August 2010
| HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
The BMC is currently testing the Beckman SPRIworks library preparation system. This platform is a fully automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. With the exception of Enrichment, it performs all of the traditional Illumina protocol steps listed on the Illumina Library Preparation page. A total of 10 libraries may be simultaneously prepared in 5 hours for Illumina sequencing.
How it Works
The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all of the reagents required to prepare a single library.
This platform utilizes AMPureXP DNA binding magnetic beads to purify fragmented DNA and perform size selection, replacing the need for column purification and gel-based size selection. There are two size range options available, 100-400bp or 300-600bp. There is also the choice to perform no size selection. After the size selection and adapter ligation process is complete on the SPRI-TE, samples will be ready for PCR Enrichment and clean-up to complete the library prep process.
For more information on the SPRI-Works system please visit www.spriworks.com
Sample Submission
All samples submitted for direct SPRI-Works library preparation must be submitted with user supplied adapter mix. Please note that samples will need to be resubmitted separately for Illumina sequencing.
