BioMicroCenter:RTPCR for Solexa: Difference between revisions
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[[Image:BioMicroCenter_RTPCR_std1.jpg|400px]] | [[Image:BioMicroCenter_RTPCR_std1.jpg|400px]] | ||
Simultaneous test. Samples were tested for RT-PCR and | Simultaneous test. Samples were tested for RT-PCR and clustered for sequencing on the same day. Their DNA concentration and the correlation of the concentration as measured by RT-PCR was then compared to their cluster number. All DNA samples had been thought to be at 10nM. <br> | ||
[[Image:|400px]] | [[Image:BioMicroCenter-QPCR_proof_of_concept.jpg|400px]] | ||
Each sample is individually colored and represented as two replicates. | Each sample is individually colored and represented as two replicates. | ||
Revision as of 15:05, 3 February 2009
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Testing of RT-PCR assay to control DNA concentration for Solexa sequencing
Standard curve using serial dilution of PhiX control DNA.
Simultaneous test. Samples were tested for RT-PCR and clustered for sequencing on the same day. Their DNA concentration and the correlation of the concentration as measured by RT-PCR was then compared to their cluster number. All DNA samples had been thought to be at 10nM.
Each sample is individually colored and represented as two replicates.
