BioMicroCenter:RTPCR: Difference between revisions
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== [[BioMicroCenter:BioMark | Fluidigm BioMark-UNAVAILABLE- Contact BMC for details | == [[BioMicroCenter:BioMark | Fluidigm BioMark-UNAVAILABLE- Contact BMC for details ]] == | ||
[[Image:BioMicroCenter_BioMarkChip.jpg|left]] | [[Image:BioMicroCenter_BioMarkChip.jpg|left]] | ||
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Using the BioMark system requires some additional processing over traditional RT-PCR. Using only 10nL in each reaction, ensuring that each reaction has sufficient template is a primary concern. To create a concentrated enough template, the BioMark system requires a pre-amplification where all of the primer pairs to be tested are mixed together with each sample and amplified by standard PCR. This increases the concentration of the specific product enough to ensure that sufficient template is added to each of the final reactions. | Using the BioMark system requires some additional processing over traditional RT-PCR. Using only 10nL in each reaction, ensuring that each reaction has sufficient template is a primary concern. To create a concentrated enough template, the BioMark system requires a pre-amplification where all of the primer pairs to be tested are mixed together with each sample and amplified by standard PCR. This increases the concentration of the specific product enough to ensure that sufficient template is added to each of the final reactions. | ||
* <B>[https://mit-ki.ilabsolutions.com/equipment/show/261686/?tab=schedule Schedule time on the Biomark]</B><BR><BR> | * <B>[https://mit-ki.ilabsolutions.com/equipment/show/261686/?tab=schedule Schedule time on the Biomark-UNAVAILABLE- Contact BMC for details ]</B><BR><BR> | ||
Use of the BioMark costs '''$150''' per use to help defray the service contract charge. | Use of the BioMark costs '''$150''' per use to help defray the service contract charge. | ||
Revision as of 07:55, 7 April 2016
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Fluidigm BioMark-UNAVAILABLE- Contact BMC for details
The Fluidigm BioMark system is a highly parallelized system allowing for running thousands of RT-PCR reactions on a single cartridge. Useful for genotyping, gene expression analysis and digital PCR, the BioMark cassette works using thousands of NanoFlex(TM) valves to fill thousands of individual reactions from a matrix of samples and primers. Standard Geneotyping / Gene Expression cassettes come in 48x48 and 96x96 format allowing either ~2,500 or ~10,000 reactions on a single cassette. RT-PCR in the BioMark system is compatible with TaqMan(TM), the Roche Universal priming kit, and SYBR Green technologies.
Using the BioMark system requires some additional processing over traditional RT-PCR. Using only 10nL in each reaction, ensuring that each reaction has sufficient template is a primary concern. To create a concentrated enough template, the BioMark system requires a pre-amplification where all of the primer pairs to be tested are mixed together with each sample and amplified by standard PCR. This increases the concentration of the specific product enough to ensure that sufficient template is added to each of the final reactions.
Use of the BioMark costs $150 per use to help defray the service contract charge. For training please email biomicro@mit.edu
Traditional Real Time PCR
Light Cycler 480 II Real-time PCR Machines
The LightCycler 480 Real-Time PCR System is a fully integrated multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids. Building on the benefits of Roche's capillary-based LightCycler® Systems, it goes one step further in offering enhanced throughput, compatibility with automation equipment and maximum flexibility regarding hard- and software. Providing novel ways to combine speed and accuracy without compromises, the LightCycler® 480 Real-Time PCR System meets the needs of a broad range of applications in research fields such as gene expression studies, discovery and analysis of genetic variation or array data validation.
There is a charge per plate to cover the cost of the service contract.
96 well plates and SYBR Green can also be supplied on demand for use on the LightCycler480
Please Note: There are three LightCycler systems (Thing 1, Thing 2, and Thing 3) that have a sign up maximum of four hours per day during business hours (10AM to 5PM), all time slots are available the night before even if it exceeds the maximum.
