BioMicroCenter:Protocols

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Revision as of 17:36, 22 April 2009 (view source) Allison Perrotta (Talk | contribs) (→ILLUMINA SEQUENCING PROTOCOLS) ← Previous diff		Revision as of 16:12, 2 June 2009 (view source) Allison Perrotta (Talk | contribs) (→ILLUMINA SEQUENCING PROTOCOLS) Next diff →
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	== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING PROTOCOLS]] ==		== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING PROTOCOLS]] ==
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		+	[[Media:BioMicro_Illumina_Sequence_Information_for_Customers_DEC2008.pdf | Illumina Adapter and Primer Sequence Information]]
	<B><BIG> Cluster Station and Genome Analyzer </BIG></B>		<B><BIG> Cluster Station and Genome Analyzer </BIG></B>

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Revision as of 16:12, 2 June 2009

BioMicro Center Protocol Testing

• RT-PCR Protocol for Sample QC
• Cluster Visualization Protocol for Flowcell QC

ILLUMINA SEQUENCING PROTOCOLS

Illumina Adapter and Primer Sequence Information

Cluster Station and Genome Analyzer

• Priming and Single Rread Protocol
• Priming and Paired-End Protocol

ChIP Seq

• Illumina Protocol

Digital Gene Expression

• Illumina Protocol (NlaIII, full kit protocol)

• Illumina Protocol (NlaIII, oligos only protocol)- unlike the full kit, this kit provides only the adapters PCR primers. Users wishing to adapting current lab protocols may find this protocol more useful.

• Illumina Protocol (DpnII, full kit protocol)

• Illumina Protocol (DpnII, olgios only protocol)

mRNA Seq

• Illumina Protocol
• Paired End Illumina Protocol

Small RNA Seq

• Tuschl Lab Protocol

• Illumina Protocol (full kit protocol)

• Illumina Protocol (oligos only protocol)

• Illumina Protocol (Alternate v1.5 protocol)

Genomic DNA

• Generic Illumina protocol

• Illumina protocol (oligos only protocol)

• Paired End Illumina Protocol

• Paired End Multiplexing Illumina Protocol

• Mate Pair Illumina Protocol (2-5kb samples)