BioMicroCenter:PricingFY2015

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(SAMPLE PREPARATION)
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  |-
  |-
  | Eukaryote (3' or Exon: >20ng)
  | Eukaryote (3' or Exon: >20ng)
-
  |align="right"| $300
+
  |align="right"| $330
-
  |align="right"| $380
+
  |align="right"| $410
-
  |align="right"| $570
+
  |align="right"| $600
  |align="center"| per sample
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
  | Using NuGEN sample labeling kit.
  |-
  |-
  | Eukaryote (<20ng)
  | Eukaryote (<20ng)
-
  |align="right"| $400
+
  |align="right"| $430
-
  |align="right"| $500
+
  |align="right"| $530
-
  |align="right"| $750
+
  |align="right"| $780
  |align="center"| per sample
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
  | Using NuGEN sample labeling kit.
  |-
  |-
  | Prokaryote
  | Prokaryote
-
  |align="right"| $365
+
  |align="right"| $400
-
  |align="right"| $455
+
  |align="right"| $480
-
  |align="right"| $683
+
  |align="right"| $700
  |align="center"| per sample
  |align="center"| per sample
  | Using Ambion sample labeling kit.   
  | Using Ambion sample labeling kit.   
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  | colspan="6" | '''AGILENT PROCESSING'''
  | colspan="6" | '''AGILENT PROCESSING'''
  |-
  |-
-
  | Expression - ONE COLOR - Eukaryote
+
  | Expression - ONE COLOR  
-
  |align="right"| $200
+
  |align="right"| $225
-
  |align="right"| $250
+
  |align="right"| $275
-
  |align="right"| $375
+
  |align="right"| $400
  |align="center"| per array
  |align="center"| per array
  |  
  |  
  |-
  |-
-
  | Expression - TWO COLOR - Eukaryote
+
  | Expression - TWO COLOR  
-
  |align="right"| $290
+
  |align="right"| $325
-
  |align="right"| $340
+
  |align="right"| $375
-
  |align="right"| $510
+
  |align="right"| $550
  |align="center"| per array
  |align="center"| per array
  | Includes both Cy5 and Cy3
  | Includes both Cy5 and Cy3
-
|-
 
-
| miRNA - ONE COLOR
 
-
|align="right"| $260
 
-
|align="right"| $320
 
-
|align="right"| $480
 
-
|align="center"| per array
 
-
|
 
-
|-
 
-
| Prokaryote - ONE COLOR
 
-
|align="right"| $200
 
-
|align="right"| $260
 
-
|align="right"| $390
 
-
|align="center"| per array
 
-
|
 
  |-  
  |-  
  | Scanning Only
  | Scanning Only
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  |-  
  |-  
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prices effective 7.1.2013
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prices effective 7.1.2014
<br>
<br>
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{| border=1
{| border=1
  ! AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER
  ! AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER
-
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]  
-
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
+
!width=80| MIT
-
  !width=100| unit
+
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
  !width=80| unit
  ! Notes
  ! Notes
  |-
  |-
  | RNA / DNA on Advanced Analytical
  | RNA / DNA on Advanced Analytical
 +
|align="right"| $8
  |align="right"| $8
  |align="right"| $8
  |align="right"| $12
  |align="right"| $12
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  | AA - Preloaded plate
  | AA - Preloaded plate
  |align="right"| $30
  |align="right"| $30
-
  |align="right"|  
+
  |align="right"| $33
 +
|align="right"| $45
  |align="center"| per row (12)
  |align="center"| per row (12)
  | 2ul per sample in each well using plates from BMC
  | 2ul per sample in each well using plates from BMC
  |-
  |-
  | All DNA & RNA on BIOANALYZER
  | All DNA & RNA on BIOANALYZER
 +
|align="right"| $15
  |align="right"| $15
  |align="right"| $15
  |align="right"| $25
  |align="right"| $25
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  | High Sensitivity protein
  | High Sensitivity protein
  |align="right"| $40
  |align="right"| $40
 +
|align="right"| $45
  |align="right"| $60
  |align="right"| $60
  |align="center"| per sample
  |align="center"| per sample
  | detection of proteins at < 30ng/ul.   
  | detection of proteins at < 30ng/ul.   
 +
|-
 +
| Self run chips
 +
|align="right"| $55-90
 +
|align="right"| request
 +
|align="right"| NA
 +
|align="center"| per chip
 +
| Price varies by flavor of Chip. 
  |-
  |-
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prices effective 7.1.2012
+
prices effective 7.1.2014
<br>.
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{| border=1
{| border=1
  ! OTHER LAB SERVICES
  ! OTHER LAB SERVICES
-
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB MIT]]
-
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
+
  !width=80| MIT
-
  !width=100| unit
+
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
  !width=80| unit
  ! SIGN UP
  ! SIGN UP
  ! Notes
  ! Notes
  |-
  |-
-
  | colspan="6" align="center"| '''FLUIDIGM'''
+
  | colspan="7" align="center"| '''FLUIDIGM'''
  |-
  |-
  | Fluidigm BioMark
  | Fluidigm BioMark
  |align="right"| $150
  |align="right"| $150
 +
|align="right"| $165
  |align="right"| $225
  |align="right"| $225
  |align="center"| per use
  |align="center"| per use
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  |-
  |-
  | 48.48 Dynamic Array Chip
  | 48.48 Dynamic Array Chip
-
  |align="right"| $250
+
  |colspan="2" align="right"| $250
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per cassette
  |align="center"| per cassette
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  |-
  |-
  | 96.96 Dynamic Array Chip
  | 96.96 Dynamic Array Chip
-
  |align="right"| $700
+
  |colspan="2" align="right"| $700
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per cassette
  |align="center"| per cassette
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  |-
  |-
  | WAFERGEN SMART CHIP
  | WAFERGEN SMART CHIP
-
  |align="right"| $150
+
  |colspan="2" align="right"| $150
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per chip
  |align="center"| per chip
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  | WAFERGEN TRAINING
  | WAFERGEN TRAINING
  |align="right"| $100
  |align="right"| $100
 +
|align="right"| $110
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per chip
  |align="center"| per chip
  | Mandatory
  | Mandatory
  | Contact [[BioMicroCenter:People|Michael]]
  | Contact [[BioMicroCenter:People|Michael]]
-
  |- | colspan="6" align="center"| '''RT-PCR'''
+
  |-  
 +
| colspan="7" align="center"| '''RT-PCR'''
  |-
  |-
  | Roche LightCycler 480
  | Roche LightCycler 480
-
  |align="right"| $10
+
  |align="right"| $15
 +
|align="right"| $20
  |align="right"| NA
  |align="right"| NA
  |align="center"| per plate
  |align="center"| per plate
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  |-
  |-
  | SYBR Green (KAPA/Roche)
  | SYBR Green (KAPA/Roche)
-
  |align="right"| $35
+
  |colspan="2"  align="right"| $35
  |align="right"| NA
  |align="right"| NA
  |align="center"| per ml
  |align="center"| per ml
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  |-
  |-
  | 96-well PLATES
  | 96-well PLATES
-
  |align="right"| $6
+
  |colspan="2" align="right"| $6
  |align="right"| NA
  |align="right"| NA
  |align="center"| per plate
  |align="center"| per plate
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  |-
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prices effective 7.1.2013
+
prices effective 7.1.2014
.
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<br>
<br>

Revision as of 11:39, 26 April 2014

Image:BioMicroCenter-header6.jpg

Contents

ILLUMINA SEQUENCING

DRAFT - NOT FOR USE

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the HiSeq.

ILLUMINA SEQUENCING CORE LAB MIT Non-MIT unit Notes

HiSEQ 2000 sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
Single End (40nt) $1,085 $1,195 $1,628 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Paired End (40+40nt) $1,625 $1,788 $2,438 per lane
Add'l 40nt $300 $330 $450 per lane

MiSeq v2 sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
70nt Run $1,150 $1,265 $1,725 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
300nt Run (PE or SE) $1,350 $1,485 $2,025 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
500nt Run (PE or SE) $1,600 $1,760 $2,400 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

MiSeq v3 sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
600nt Run (PE or SE) $2,050 $2,255 $3,075 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

GAII / GAIIx sequencing (Average ~25-40m reads/lane)
GAII Services are being phased out in FY2015. Requests that must be processed on the GAII will be priced per experiment.

Additional Services
These services apply to samples submitted for all platforms.
Sample Multiplexing $50 $55 $75 per 10 samples Combined over the whole order. Only applies to samples combined by BMC technicians.
Add'l Repriming (HiSeq only) $200 $220 $300 per flow cell Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Quality Control Only $25 $28 $38 per sample Includes RT-PCR and BioAnalyzer



Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.


SAMPLE PREPARATION

ILLUMINA Sample Preparation CORE LAB MIT Non-MIT unit Notes
STARTING WITH DNA
Full Service Sheared DNA (SPRIWORKS) $150 $165 $225 per sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only $100 $110 N/A per sample User must provide all primers. Samples will be returned for amplification.
Intact Genomic DNA (NEXTERA) - 50ng input $175 $193 $263 per sample Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input $100 $110 $150 per sample Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
16S Amplicon $1,200 $1,320 $1,800 per 48 sample Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
Other Amplicon $960 $1,056 $1,440 per 48 sample Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
Samples *must* be submitted amplified with appropriate linkers.
STARTING WITH RNA
Illumina TruSeq $300 $330 $450 per sample Preformed using TruSeq AND SPRIworks system.
Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+strand specific $30 $33 $45 per sample Requires 1ug of RNA. Uses the dUTP method.
Epicenter Ribozero
no polyA pulldown
$350 $385 $525 per sample Requires 1ug of RNA. Based on Illumina Truseq chemistry
Low Input (Clontech) $400 $440 $600 per sample Can work on single cells
Low Input (NuGen) $475 $523 $713 per sample Can work on degraded RNA.
Tight Size Cut $100 $110 $150 per pool +/- 40bp using PippinPrep
Small RNA $275 $303 $413 per sample Uses NEB small RNA kit
STARTING WITH CHROMATIN Note: User must supply antibodies.
Full Service ChIP-seq $210 $231 $315 per sample Preformed using IP-Star AND SPRIworks system.
Includes ChIP, library contruction and barcoding.
IP-Star Only $300 $330 $450 per batch (1-8 samples) Preformed using IP-Star

.




MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS CORE LAB MIT Non-MIT unit Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng) $330 $410 $600 per sample Using NuGEN sample labeling kit.
Eukaryote (<20ng) $430 $530 $780 per sample Using NuGEN sample labeling kit.
Prokaryote $400 $480 $700 per sample Using Ambion sample labeling kit.
AGILENT PROCESSING
Expression - ONE COLOR $225 $275 $400 per array
Expression - TWO COLOR $325 $375 $550 per array Includes both Cy5 and Cy3
Scanning Only $50 $50 N/A per slide Price per glass slide.

prices effective 7.1.2014

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices
  • available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER CORE LAB MIT Non-MIT unit Notes
RNA / DNA on Advanced Analytical $8 $8 $12 per sample
AA - Preloaded plate $30 $33 $45 per row (12) 2ul per sample in each well using plates from BMC
All DNA & RNA on BIOANALYZER $15 $15 $25 per sample
High Sensitivity protein $40 $45 $60 per sample detection of proteins at < 30ng/ul.
Self run chips $55-90 request NA per chip Price varies by flavor of Chip.

prices effective 7.1.2014
.

REAL TIME PCR

OTHER LAB SERVICES CORE LAB MIT MIT Non-MIT unit SIGN UP Notes
FLUIDIGM
Fluidigm BioMark $150 $165 $225 per use CALENDER Contact Shmulik
48.48 Dynamic Array Chip $250 N/A per cassette Cassettes are available in the BMC.
96.96 Dynamic Array Chip $700 N/A per cassette Cassettes are available in the BMC.
WAFERGEN SMART CHIP $150 N/A per chip Chips available from BMC Contact Michael
WAFERGEN TRAINING $100 $110 N/A per chip Mandatory Contact Michael
RT-PCR
Roche LightCycler 480 $15 $20 NA per plate CALENDER *available for CORE only Contact Scott for training
SYBR Green (KAPA/Roche) $35 NA per ml Available for MIT only
96-well PLATES $6 NA per plate Available for MIT only

prices effective 7.1.2014 .

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
PIPPIN PREP
Gel Isolation $50 $75 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Shmulik for training.
PLATE READER
Varioskan $10 $15 per session CALENDER Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Shmulik for training
ROBOTICS
Tecan EVO 150 $60 $150 per 2h block - based on scheduled time. CALENDER
non-filter tips $6.00 $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $100 $150 per session Contact Stuart
NANODROP
Nanodrop -- -- Walk up service * no charge

prices effective 7.1.2013

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES CORE LAB Other MIT / Non-MIT unit Notes
Informatics Support $70 $90 / $135 per hour Limited support for non-MIT users
Informatics Share $960 N/A per 0.1FTE MIT only. Priority access to informatics.
Data storage (1 year) $280 N/A per TB
ROUS node (1 year) $400 N/A 8 CPU Provides a priority queue for the lab on ROUS.
Spotfire License (1 year) Contact Stephen Goldman N/A per user (Limited seats available)
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