BioMicroCenter:PricingFY2015

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(New page: {{BioMicroCenter}} == ILLUMINA SEQUENCING == DRAFT - NOT FOR USE === SEQUENCING === Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''COR...)
Current revision (12:22, 27 April 2014) (view source)
(COMPUTATIONAL SERVICES)
 
(5 intermediate revisions not shown.)
Line 7: Line 7:
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq.
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq.
-
{| border=1 align="left"
+
{| border=1  
-
  ! ILLUMINA SEQUENCING:HiSeq
+
  ! ILLUMINA SEQUENCING
-
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
-
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
+
!width=80| MIT
-
  !width=100| unit
+
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
  !width=80| unit
  ! Notes
  ! Notes
  |-
  |-
-
  | colspan="5" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
+
  | colspan="6" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
  |-
  |-
  | Single End (40nt)
  | Single End (40nt)
  |align="right"| $1,085
  |align="right"| $1,085
 +
|align="right"| $1,195
  |align="right"| $1,628
  |align="right"| $1,628
  |align="center"| per lane
  |align="center"| per lane
Line 24: Line 26:
  | Paired End (40+40nt)
  | Paired End (40+40nt)
  |align="right"| $1,625
  |align="right"| $1,625
 +
|align="right"| $1,788
  |align="right"| $2,438
  |align="right"| $2,438
  |align="center"| per lane
  |align="center"| per lane
Line 30: Line 33:
  | Add'l 40nt
  | Add'l 40nt
  |align="right"| $300
  |align="right"| $300
 +
|align="right"| $330
  |align="right"| $450
  |align="right"| $450
  |align="center"| per lane
  |align="center"| per lane
-
  |
+
  |
-
  |-
+
  |-  
-
  | colspan="5" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
+
  | colspan="6" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
  |-
  |-
  | 70nt Run
  | 70nt Run
-
  |align="right"| $1,100
+
  |align="right"| $1,150
-
  |align="right"| $1,650
+
  |align="right"| $1,265
 +
|align="right"| $1,725
  |align="center"| per lane
  |align="center"| per lane
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
  | 300nt Run (PE or SE)
  | 300nt Run (PE or SE)
-
  |align="right"| $1,300
+
  |align="right"| $1,350
-
  |align="right"| $1,950
+
  |align="right"| $1,485
 +
|align="right"| $2,025
  |align="center"| per lane
  |align="center"| per lane
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
  | 500nt Run (PE or SE)
  | 500nt Run (PE or SE)
-
  |align="right"| $1,500
+
  |align="right"| $1,600
-
  |align="right"| $2,250
+
|align="right"| $1,760
 +
  |align="right"| $2,400
  |align="center"| per lane
  |align="center"| per lane
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
-
  | colspan="5" align=center |<BR>'''MiSeq v3''' sequencing (minimum 8m reads/lane. Expected ~25m reads/lane) -- minimum will rise
+
  | colspan="6" align=center |<BR>'''MiSeq v3''' sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
-
|-
+
-
| 150nt Run
+
-
|align="right"| $1,300
+
-
|align="right"| $1,950
+
-
|align="center"| per lane
+
-
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
+
  |-
  |-
  | 600nt Run (PE or SE)
  | 600nt Run (PE or SE)
-
  |align="right"| $1,800
+
  |align="right"| $2,050
-
  |align="right"| $2,700
+
  |align="right"| $2,255
 +
|align="right"| $3,075
  |align="center"| per lane
  |align="center"| per lane
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
-
  | colspan="5" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
+
  | colspan="6" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
  |-
  |-
-
  | colspan="5" align=center |''GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.''
+
  | colspan="6" align=center |GAII Services are being phased out in FY2015. Requests that must be processed on the GAII will be priced per experiment.
-
|-
+
-
| 8 Lanes (SE/PE)
+
-
|align="right"| $5,200 / $7,550
+
-
|align="right"| $7,800 / $11,325
+
-
|align="center"| 1 day
+
-
| 24nt per day. 45nt per seq kit.
+
-
|-
+
-
| 4 Lanes (SE/PE)
+
-
|align="right"| $3,800 / $5,075
+
-
|align="right"| $5,700 / $7,613
+
-
|align="center"| 1 day
+
-
| 36nt per day. 66nt per seq kit.
+
-
|-
+
-
| 2 Lanes (SE/PE)
+
-
|align="right"| $3,100 / $4,375
+
-
|align="right"| $4,650 / $6,563
+
-
|align="center"| 1 day
+
-
| 48nt per day. 102nt per seq kit.
+
-
|-
+
-
| 1 Lane (SE/PE)
+
-
|align="right"| $2,750 / NA
+
-
|align="right"| $4,125 / NA
+
-
|align="center"| 1 day
+
-
| 72nt per day. 140nt per seq kit.
+
-
|-
+
-
| Add'l Day
+
-
|align="right"| $600
+
-
|align="right"| $900
+
-
|align="center"| 1 day
+
-
| covers service contract and data storage requirements.
+
-
|-
+
-
| Add'l Sequencing Kit
+
-
|align="right"| $1,450
+
-
|align="right"| $2,175
+
-
|align="center"| 1 Kit
+
-
|
+
  |-
  |-
-
  | colspan="5" align=center |<BR>'''Additional Services'''  
+
  | colspan="6" align=center |<BR>'''Additional Services'''  
  |-  
  |-  
-
  | colspan="5" align=center |''These services apply to samples submitted for all platforms.''
+
  | colspan="6" align=center |''These services apply to samples submitted for all platforms.''
  |-  
  |-  
  | Sample Multiplexing
  | Sample Multiplexing
  |align="right"| $50
  |align="right"| $50
 +
|align="right"| $55
  |align="right"| $75
  |align="right"| $75
  |align="center"| per 10 samples
  |align="center"| per 10 samples
  | Combined over the whole order. Only applies to samples combined by BMC technicians.
  | Combined over the whole order. Only applies to samples combined by BMC technicians.
  |-
  |-
-
  | Add'l Repriming
+
  | Add'l Repriming (HiSeq only)
  |align="right"| $200
  |align="right"| $200
 +
|align="right"| $220
  |align="right"| $300
  |align="right"| $300
  |align="center"| per flow cell
  |align="center"| per flow cell
Line 126: Line 94:
  | Quality Control Only
  | Quality Control Only
  |align="right"| $25
  |align="right"| $25
 +
|align="right"| $28
  |align="right"| $38
  |align="right"| $38
  |align="center"| per sample
  |align="center"| per sample
  | Includes RT-PCR and BioAnalyzer
  | Includes RT-PCR and BioAnalyzer
-
|}
+
|}
-
<BR>
+
 
 +
<BR><BR>
 +
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR>
-
<BR>
 
-
Prices effective 4.1.2014 <BR><BR>
 
-
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Hi-Seq. The BioMicro Center reserves the right to reject samples for sample preparations.
 
=== SAMPLE PREPARATION ===
=== SAMPLE PREPARATION ===
-
{| border=1 align="left"
+
{| border=1  
  ! ILLUMINA Sample Preparation
  ! ILLUMINA Sample Preparation
-
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
-
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
+
!width=80| MIT
-
  !width=100| unit
+
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
  !width=80| unit
  ! Notes
  ! Notes
  |-
  |-
-
  | colspan="5" | '''STARTING WITH DNA'''  
+
  | colspan="6" | '''STARTING WITH DNA'''  
  |-
  |-
  | Full Service Sheared DNA (SPRIWORKS)
  | Full Service Sheared DNA (SPRIWORKS)
  |align="right"| $150
  |align="right"| $150
 +
|align="right"| $165
  |align="right"| $225
  |align="right"| $225
  |align="center"| per sample
  |align="center"| per sample
Line 155: Line 125:
  | ''SPRI-TE use only''
  | ''SPRI-TE use only''
  |align="right"| $100
  |align="right"| $100
 +
|align="right"| $110
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per sample
  |align="center"| per sample
  | User must provide all primers. Samples will be returned for amplification.
  | User must provide all primers. Samples will be returned for amplification.
  |-
  |-
-
  | Full Service Intact Genomic DNA (NEXTERA)
+
  | Intact Genomic DNA (NEXTERA) - 50ng input
 +
|align="right"| $175
 +
|align="right"| $193
 +
|align="right"| $263
 +
|align="center"| per sample
 +
| Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
| Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input
 +
|align="right"| $100
 +
|align="right"| $110
  |align="right"| $150
  |align="right"| $150
-
|align="right"| $225
 
  |align="center"| per sample
  |align="center"| per sample
-
  | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+
  | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
| 16S Amplicon
 +
|align="right"| $1,200
 +
|align="right"| $1,320
 +
|align="right"| $1,800
 +
|align="center"| per 48 sample
 +
| Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
| Other Amplicon
 +
|align="right"| $960
 +
|align="right"| $1,056
 +
|align="right"| $1,440
 +
|align="center"| per 48 sample
 +
| Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). <BR> Samples *must* be submitted amplified with appropriate linkers.
  |-
  |-
-
  | colspan="5" | '''STARTING WITH RNA'''  
+
  | colspan="6" | '''STARTING WITH RNA'''  
  |-
  |-
  | Illumina TruSeq  
  | Illumina TruSeq  
  |align="right"| $300
  |align="right"| $300
 +
|align="right"| $330
  |align="right"| $450
  |align="right"| $450
  |align="center"| per sample
  |align="center"| per sample
Line 175: Line 169:
  |''+strand specific''
  |''+strand specific''
  |align="right"| $30
  |align="right"| $30
 +
|align="right"| $33
  |align="right"| $45
  |align="right"| $45
  |align="center"| per sample
  |align="center"| per sample
Line 181: Line 176:
  | Epicenter Ribozero<br>''no polyA pulldown''
  | Epicenter Ribozero<br>''no polyA pulldown''
  |align="right"| $350
  |align="right"| $350
 +
|align="right"| $385
  |align="right"| $525
  |align="right"| $525
  |align="center"| per sample
  |align="center"| per sample
Line 187: Line 183:
  | Low Input (Clontech)
  | Low Input (Clontech)
  |align="right"| $400
  |align="right"| $400
 +
|align="right"| $440
  |align="right"| $600
  |align="right"| $600
  |align="center"| per sample
  |align="center"| per sample
Line 192: Line 189:
  |-
  |-
  | Low Input (NuGen)
  | Low Input (NuGen)
-
  |align="right"| $430
+
  |align="right"| $475
-
  |align="right"| $645
+
  |align="right"| $523
 +
|align="right"| $713
  |align="center"| per sample
  |align="center"| per sample
  | Can work on degraded RNA.
  | Can work on degraded RNA.
  |-
  |-
  | Tight Size Cut
  | Tight Size Cut
-
  |align="right"| $85
+
  |align="right"| $100
-
  |align="right"| $130
+
  |align="right"| $110
 +
|align="right"| $150
  |align="center"| per pool
  |align="center"| per pool
  | +/- 40bp using PippinPrep
  | +/- 40bp using PippinPrep
Line 205: Line 204:
  | Small RNA
  | Small RNA
  | align="right"|$275
  | align="right"|$275
 +
| align="right"|$303
  | align="right"|$413
  | align="right"|$413
  | align="center"|per sample
  | align="center"|per sample
  | Uses NEB small RNA kit
  | Uses NEB small RNA kit
  |-
  |-
-
  | colspan="5" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
+
  | colspan="6" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
  |-
  |-
  | Full Service ChIP-seq
  | Full Service ChIP-seq
  |align="right"| $210
  |align="right"| $210
 +
|align="right"| $231
  |align="right"| $315
  |align="right"| $315
  |align="center"| per sample
  |align="center"| per sample
Line 219: Line 220:
  | ''IP-Star Only''
  | ''IP-Star Only''
  |align="right"| $300
  |align="right"| $300
 +
|align="right"| $330
  |align="right"| $450
  |align="right"| $450
  |align="center"| per batch (1-8 samples)
  |align="center"| per batch (1-8 samples)
Line 224: Line 226:
  |-
  |-
|}
|}
-
<BR>
+
 
-
<BR>
+
-
<BR>
+
.
.
<BR>
<BR>
Line 248: Line 248:
  |-
  |-
  | Eukaryote (3' or Exon: >20ng)
  | Eukaryote (3' or Exon: >20ng)
-
  |align="right"| $300
+
  |align="right"| $330
-
  |align="right"| $380
+
  |align="right"| $410
-
  |align="right"| $570
+
  |align="right"| $600
  |align="center"| per sample
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
  | Using NuGEN sample labeling kit.
  |-
  |-
  | Eukaryote (<20ng)
  | Eukaryote (<20ng)
-
  |align="right"| $400
+
  |align="right"| $430
-
  |align="right"| $500
+
  |align="right"| $530
-
  |align="right"| $750
+
  |align="right"| $780
  |align="center"| per sample
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
  | Using NuGEN sample labeling kit.
  |-
  |-
  | Prokaryote
  | Prokaryote
-
  |align="right"| $365
+
  |align="right"| $400
-
  |align="right"| $455
+
  |align="right"| $480
-
  |align="right"| $683
+
  |align="right"| $700
  |align="center"| per sample
  |align="center"| per sample
  | Using Ambion sample labeling kit.   
  | Using Ambion sample labeling kit.   
Line 270: Line 270:
  | colspan="6" | '''AGILENT PROCESSING'''
  | colspan="6" | '''AGILENT PROCESSING'''
  |-
  |-
-
  | Expression - ONE COLOR - Eukaryote
+
  | Expression - ONE COLOR  
-
  |align="right"| $200
+
  |align="right"| $225
-
  |align="right"| $250
+
  |align="right"| $275
-
  |align="right"| $375
+
  |align="right"| $400
  |align="center"| per array
  |align="center"| per array
  |  
  |  
  |-
  |-
-
  | Expression - TWO COLOR - Eukaryote
+
  | Expression - TWO COLOR  
-
  |align="right"| $290
+
  |align="right"| $325
-
  |align="right"| $340
+
  |align="right"| $375
-
  |align="right"| $510
+
  |align="right"| $550
  |align="center"| per array
  |align="center"| per array
  | Includes both Cy5 and Cy3
  | Includes both Cy5 and Cy3
-
|-
 
-
| miRNA - ONE COLOR
 
-
|align="right"| $260
 
-
|align="right"| $320
 
-
|align="right"| $480
 
-
|align="center"| per array
 
-
|
 
-
|-
 
-
| Prokaryote - ONE COLOR
 
-
|align="right"| $200
 
-
|align="right"| $260
 
-
|align="right"| $390
 
-
|align="center"| per array
 
-
|
 
  |-  
  |-  
  | Scanning Only
  | Scanning Only
Line 306: Line 292:
  |-  
  |-  
|}
|}
-
prices effective 7.1.2013
+
prices effective 7.1.2014
<br>
<br>
Line 320: Line 306:
{| border=1
{| border=1
  ! AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER
  ! AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER
-
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]  
-
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
+
!width=80| MIT
-
  !width=100| unit
+
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
  !width=80| unit
  ! Notes
  ! Notes
  |-
  |-
  | RNA / DNA on Advanced Analytical
  | RNA / DNA on Advanced Analytical
 +
|align="right"| $8
  |align="right"| $8
  |align="right"| $8
  |align="right"| $12
  |align="right"| $12
Line 333: Line 321:
  | AA - Preloaded plate
  | AA - Preloaded plate
  |align="right"| $30
  |align="right"| $30
-
  |align="right"|  
+
  |align="right"| $33
 +
|align="right"| $45
  |align="center"| per row (12)
  |align="center"| per row (12)
  | 2ul per sample in each well using plates from BMC
  | 2ul per sample in each well using plates from BMC
  |-
  |-
  | All DNA & RNA on BIOANALYZER
  | All DNA & RNA on BIOANALYZER
 +
|align="right"| $15
  |align="right"| $15
  |align="right"| $15
  |align="right"| $25
  |align="right"| $25
Line 345: Line 335:
  | High Sensitivity protein
  | High Sensitivity protein
  |align="right"| $40
  |align="right"| $40
 +
|align="right"| $45
  |align="right"| $60
  |align="right"| $60
  |align="center"| per sample
  |align="center"| per sample
  | detection of proteins at < 30ng/ul.   
  | detection of proteins at < 30ng/ul.   
 +
|-
 +
| Self run chips
 +
|align="right"| $55-90
 +
|align="right"| request
 +
|align="right"| NA
 +
|align="center"| per chip
 +
| Price varies by flavor of Chip. 
  |-
  |-
|}
|}
-
prices effective 7.1.2012
+
prices effective 7.1.2014
<br>.
<br>.
Line 357: Line 355:
{| border=1
{| border=1
  ! OTHER LAB SERVICES
  ! OTHER LAB SERVICES
-
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB MIT]]
-
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
+
  !width=80| MIT
-
  !width=100| unit
+
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
  !width=80| unit
  ! SIGN UP
  ! SIGN UP
  ! Notes
  ! Notes
  |-
  |-
-
  | colspan="6" align="center"| '''FLUIDIGM'''
+
  | colspan="7" align="center"| '''FLUIDIGM'''
  |-
  |-
  | Fluidigm BioMark
  | Fluidigm BioMark
  |align="right"| $150
  |align="right"| $150
 +
|align="right"| $165
  |align="right"| $225
  |align="right"| $225
  |align="center"| per use
  |align="center"| per use
Line 373: Line 373:
  |-
  |-
  | 48.48 Dynamic Array Chip
  | 48.48 Dynamic Array Chip
-
  |align="right"| $250
+
  |colspan="2" align="right"| $250
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per cassette
  |align="center"| per cassette
Line 380: Line 380:
  |-
  |-
  | 96.96 Dynamic Array Chip
  | 96.96 Dynamic Array Chip
-
  |align="right"| $700
+
  |colspan="2" align="right"| $700
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per cassette
  |align="center"| per cassette
Line 387: Line 387:
  |-
  |-
  | WAFERGEN SMART CHIP
  | WAFERGEN SMART CHIP
-
  |align="right"| $150
+
  |colspan="2" align="right"| $150
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per chip
  |align="center"| per chip
Line 395: Line 395:
  | WAFERGEN TRAINING
  | WAFERGEN TRAINING
  |align="right"| $100
  |align="right"| $100
 +
|align="right"| $110
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per chip
  |align="center"| per chip
  | Mandatory
  | Mandatory
  | Contact [[BioMicroCenter:People|Michael]]
  | Contact [[BioMicroCenter:People|Michael]]
-
  |- | colspan="6" align="center"| '''RT-PCR'''
+
  |-  
 +
| colspan="7" align="center"| '''RT-PCR'''
  |-
  |-
  | Roche LightCycler 480
  | Roche LightCycler 480
-
  |align="right"| $10
+
  |align="right"| $15
 +
|align="right"| $20
  |align="right"| NA
  |align="right"| NA
  |align="center"| per plate
  |align="center"| per plate
Line 409: Line 412:
  |-
  |-
  | SYBR Green (KAPA/Roche)
  | SYBR Green (KAPA/Roche)
-
  |align="right"| $35
+
  |colspan="2"  align="right"| $35
  |align="right"| NA
  |align="right"| NA
  |align="center"| per ml
  |align="center"| per ml
Line 416: Line 419:
  |-
  |-
  | 96-well PLATES
  | 96-well PLATES
-
  |align="right"| $6
+
  |colspan="2" align="right"| $6
  |align="right"| NA
  |align="right"| NA
  |align="center"| per plate
  |align="center"| per plate
Line 423: Line 426:
  |-
  |-
|}
|}
-
prices effective 7.1.2013
+
prices effective 7.1.2014
.
.
<br>
<br>
Line 431: Line 434:
{| border=1
{| border=1
  ! OTHER LAB SERVICES
  ! OTHER LAB SERVICES
-
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
-
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
+
  !width=80| MIT
-
  !width=100| unit
+
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
  ! unit
  ! SIGN UP
  ! SIGN UP
  ! Notes
  ! Notes
  |-
  |-
-
  | colspan="6" align="center"| '''PIPPIN PREP'''
+
  | colspan="7" align="center"| '''PIPPIN PREP'''
  |-
  |-
  |Gel Isolation
  |Gel Isolation
  |align="right"| $50
  |align="right"| $50
 +
|align="right"| $55
  |align="right"| $75
  |align="right"| $75
  |align="center"| per gel (5 lanes)
  |align="center"| per gel (5 lanes)
-
  | TBA
+
  | email biomicro
  |  
  |  
  |-
  |-
  |Training
  |Training
  |align="right"| $25
  |align="right"| $25
 +
|align="right"| $30
  |align="right"| $50
  |align="right"| $50
  |align="center"| per session
  |align="center"| per session
-
  | TBA
+
  | email biomicro
  | Contact [[BioMicroCenter:People|Shmulik]] for training.
  | Contact [[BioMicroCenter:People|Shmulik]] for training.
  |-
  |-
-
| colspan="6" align="center"| '''PLATE READER'''
+
| colspan="7" align="center"| '''PLATE READER'''
  |-
  |-
  | Varioskan
  | Varioskan
 +
|align="right"| $12
 +
|align="right"| $13.2
 +
|align="right"| $18
 +
|align="center"| per HOUR
 +
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
 +
| Quick scans (those requiring less then ''20'' minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Shmulik]] for training
 +
|-
 +
| colspan="7" align="center"| '''SONICATOR'''
 +
|-
 +
| Covaris S2
  |align="right"| $10
  |align="right"| $10
 +
|align="right"| $11
  |align="right"| $15
  |align="right"| $15
-
  |align="center"| per session
+
  |align="center"| per HOUR
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
-
  | Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Shmulik]] for training
+
  |  
  |-
  |-
-
  | colspan="6" align="center"| '''ROBOTICS'''
+
| Covaris Training
 +
|align="right"| $25
 +
|align="right"| $28
 +
|align="right"| $50
 +
|align="center"| per session
 +
|
 +
| Contact [[BioMicroCenter:People|Shmulik]] for training
 +
|-
 +
  | colspan="7" align="center"| '''ROBOTICS'''
  |-
  |-
  | Tecan EVO 150
  | Tecan EVO 150
  |align="right"| $60
  |align="right"| $60
 +
|align="right"| $66
  |align="right"| $150
  |align="right"| $150
  |align="center"| per 2h block - based on scheduled time.
  |align="center"| per 2h block - based on scheduled time.
 +
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
 +
|
 +
|-
 +
| Tecan EVO 150 Practice
 +
|align="right"| $10
 +
|align="right"| $11
 +
|align="right"| $150
 +
|align="center"| per 1h block - based on scheduled time.<BR> Must indicate practice on the calendar.
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  |  
  |  
  |-
  |-
  |non-filter tips
  |non-filter tips
-
  |align="right"| $6.00
+
  |colspan="3" align="center"| $6.00
-
|align="right"| $6.00
+
  |align="center"| per box
  |align="center"| per box
  |
  |
Line 479: Line 512:
  |-
  |-
  |Filter tips
  |Filter tips
-
  |align="right"| $8.00
+
  |colspan="3" align="center"| $8.00
-
|align="right"| $8.00
+
  |align="center"| per box
  |align="center"| per box
  |
  |
Line 487: Line 519:
  | Training
  | Training
  |align="right"| $100
  |align="right"| $100
 +
|align="right"| $110
  |align="right"| $150
  |align="right"| $150
  |align="center"| per session
  |align="center"| per session
Line 492: Line 525:
  |  
  |  
  |-
  |-
-
  | colspan="6" align="center"| '''NANODROP'''
+
  | colspan="7" align="center"| '''NANODROP'''
  |-
  |-
  | Nanodrop
  | Nanodrop
-
  |align="right"| --
+
  |colspan="3" align="center"| --
-
|align="right"| --
+
  |align="center"|  
  |align="center"|  
  | Walk up service
  | Walk up service
Line 502: Line 534:
  |-
  |-
|}
|}
-
prices effective 7.1.2013
+
prices effective 7.1.2014
<br><br>
<br><br>
Line 527: Line 559:
  | Data storage (1 year)
  | Data storage (1 year)
  |align="right"| $280
  |align="right"| $280
-
  |align="right"| N/A
+
  |align="right"| $400 / N/A
  |align="center"| per TB
  |align="center"| per TB
  |  
  |  
-
|-
 
-
| ROUS node (1 year)
 
-
|align="right"| $400
 
-
|align="right"| N/A
 
-
|align="center"| 8 CPU
 
-
| Provides a priority queue for the lab on ROUS.
 
-
|-
 
-
| Spotfire License (1 year)
 
-
|align="right"| ''Contact Stephen Goldman''
 
-
|align="right"| N/A
 
-
|align="center"| per user
 
-
| (Limited seats available)
 
  |-
  |-
|}
|}

Current revision

Image:BioMicroCenter-header6.jpg

Contents

ILLUMINA SEQUENCING

DRAFT - NOT FOR USE

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the HiSeq.

ILLUMINA SEQUENCING CORE LAB MIT Non-MIT unit Notes

HiSEQ 2000 sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
Single End (40nt) $1,085 $1,195 $1,628 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Paired End (40+40nt) $1,625 $1,788 $2,438 per lane
Add'l 40nt $300 $330 $450 per lane

MiSeq v2 sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
70nt Run $1,150 $1,265 $1,725 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
300nt Run (PE or SE) $1,350 $1,485 $2,025 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
500nt Run (PE or SE) $1,600 $1,760 $2,400 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

MiSeq v3 sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
600nt Run (PE or SE) $2,050 $2,255 $3,075 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

GAII / GAIIx sequencing (Average ~25-40m reads/lane)
GAII Services are being phased out in FY2015. Requests that must be processed on the GAII will be priced per experiment.

Additional Services
These services apply to samples submitted for all platforms.
Sample Multiplexing $50 $55 $75 per 10 samples Combined over the whole order. Only applies to samples combined by BMC technicians.
Add'l Repriming (HiSeq only) $200 $220 $300 per flow cell Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Quality Control Only $25 $28 $38 per sample Includes RT-PCR and BioAnalyzer



Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.


SAMPLE PREPARATION

ILLUMINA Sample Preparation CORE LAB MIT Non-MIT unit Notes
STARTING WITH DNA
Full Service Sheared DNA (SPRIWORKS) $150 $165 $225 per sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only $100 $110 N/A per sample User must provide all primers. Samples will be returned for amplification.
Intact Genomic DNA (NEXTERA) - 50ng input $175 $193 $263 per sample Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input $100 $110 $150 per sample Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
16S Amplicon $1,200 $1,320 $1,800 per 48 sample Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
Other Amplicon $960 $1,056 $1,440 per 48 sample Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
Samples *must* be submitted amplified with appropriate linkers.
STARTING WITH RNA
Illumina TruSeq $300 $330 $450 per sample Preformed using TruSeq AND SPRIworks system.
Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+strand specific $30 $33 $45 per sample Requires 1ug of RNA. Uses the dUTP method.
Epicenter Ribozero
no polyA pulldown
$350 $385 $525 per sample Requires 1ug of RNA. Based on Illumina Truseq chemistry
Low Input (Clontech) $400 $440 $600 per sample Can work on single cells
Low Input (NuGen) $475 $523 $713 per sample Can work on degraded RNA.
Tight Size Cut $100 $110 $150 per pool +/- 40bp using PippinPrep
Small RNA $275 $303 $413 per sample Uses NEB small RNA kit
STARTING WITH CHROMATIN Note: User must supply antibodies.
Full Service ChIP-seq $210 $231 $315 per sample Preformed using IP-Star AND SPRIworks system.
Includes ChIP, library contruction and barcoding.
IP-Star Only $300 $330 $450 per batch (1-8 samples) Preformed using IP-Star

.




MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS CORE LAB MIT Non-MIT unit Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng) $330 $410 $600 per sample Using NuGEN sample labeling kit.
Eukaryote (<20ng) $430 $530 $780 per sample Using NuGEN sample labeling kit.
Prokaryote $400 $480 $700 per sample Using Ambion sample labeling kit.
AGILENT PROCESSING
Expression - ONE COLOR $225 $275 $400 per array
Expression - TWO COLOR $325 $375 $550 per array Includes both Cy5 and Cy3
Scanning Only $50 $50 N/A per slide Price per glass slide.

prices effective 7.1.2014

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices
  • available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER CORE LAB MIT Non-MIT unit Notes
RNA / DNA on Advanced Analytical $8 $8 $12 per sample
AA - Preloaded plate $30 $33 $45 per row (12) 2ul per sample in each well using plates from BMC
All DNA & RNA on BIOANALYZER $15 $15 $25 per sample
High Sensitivity protein $40 $45 $60 per sample detection of proteins at < 30ng/ul.
Self run chips $55-90 request NA per chip Price varies by flavor of Chip.

prices effective 7.1.2014
.

REAL TIME PCR

OTHER LAB SERVICES CORE LAB MIT MIT Non-MIT unit SIGN UP Notes
FLUIDIGM
Fluidigm BioMark $150 $165 $225 per use CALENDER Contact Shmulik
48.48 Dynamic Array Chip $250 N/A per cassette Cassettes are available in the BMC.
96.96 Dynamic Array Chip $700 N/A per cassette Cassettes are available in the BMC.
WAFERGEN SMART CHIP $150 N/A per chip Chips available from BMC Contact Michael
WAFERGEN TRAINING $100 $110 N/A per chip Mandatory Contact Michael
RT-PCR
Roche LightCycler 480 $15 $20 NA per plate CALENDER *available for CORE only Contact Scott for training
SYBR Green (KAPA/Roche) $35 NA per ml Available for MIT only
96-well PLATES $6 NA per plate Available for MIT only

prices effective 7.1.2014 .

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB MIT Non-MIT unit SIGN UP Notes
PIPPIN PREP
Gel Isolation $50 $55 $75 per gel (5 lanes) email biomicro
Training $25 $30 $50 per session email biomicro Contact Shmulik for training.
PLATE READER
Varioskan $12 $13.2 $18 per HOUR CALENDER Quick scans (those requiring less then 20 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Shmulik for training
SONICATOR
Covaris S2 $10 $11 $15 per HOUR CALENDER
Covaris Training $25 $28 $50 per session Contact Shmulik for training
ROBOTICS
Tecan EVO 150 $60 $66 $150 per 2h block - based on scheduled time. CALENDER
Tecan EVO 150 Practice $10 $11 $150 per 1h block - based on scheduled time.
Must indicate practice on the calendar.
CALENDER
non-filter tips $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 per box
Training $100 $110 $150 per session Contact Stuart
NANODROP
Nanodrop -- Walk up service * no charge

prices effective 7.1.2014

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES CORE LAB Other MIT / Non-MIT unit Notes
Informatics Support $70 $90 / $135 per hour Limited support for non-MIT users
Informatics Share $960 N/A per 0.1FTE MIT only. Priority access to informatics.
Data storage (1 year) $280 $400 / N/A per TB
Personal tools