BioMicroCenter:Pricing

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{{BioMicroCenter}}
{{BioMicroCenter}}
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== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==
+
== SEQUENCING ==
 +
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq.
-
=== SEQUENCING ===
+
{| border=1  
-
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer.
+
  ! ILLUMINA SEQUENCING
-
 
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
-
{| border=1 align="left"
+
!width=80| MIT
-
  ! ILLUMINA SEQUENCING:HiSeq
+
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
-
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
+
  !width=80| unit
-
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
+
-
  !width=100| unit
+
  ! Notes
  ! Notes
  |-
  |-
-
  | colspan="5" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 100m reads/lane. Average ~160-240m reads/lane)
+
  | colspan="6" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
  |-
  |-
  | Single End (40nt)
  | Single End (40nt)
-
  |align="right"| $1,170
+
  |align="right"| $1,140
-
  |align="right"| $1,755
+
  |align="right"| $1,254
 +
|align="right"| $1,710
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 yrs.
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
  |-
  |-
  | Paired End (40+40nt)
  | Paired End (40+40nt)
-
  |align="right"| $1,850
+
  |align="right"| $1,710
-
  |align="right"| $2,775
+
|align="right"| $1,881
 +
  |align="right"| $2,565
  |align="center"| per lane
  |align="center"| per lane
  |  
  |  
  |-
  |-
  | Add'l 40nt
  | Add'l 40nt
-
  |align="right"| $370
+
  |align="right"| $330
-
  |align="right"| $555
+
  |align="right"| $363
 +
|align="right"| $495
  |align="center"| per lane
  |align="center"| per lane
-
  |   
+
  |
 +
  |-
 +
| colspan="6" align=center |<BR>'''NextSeq''' sequencing (minimum 250m reads/lane. maximum ~500m reads/lane)
  |-
  |-
-
  | colspan="5" align=center |<BR>'''MiSeq''' sequencing (minimum 2m reads/lane. Average ~5m reads/lane)
+
  | 91nt kit <BR> (eg. 40PE or 50SE)
 +
|align="right"| $2,100
 +
|align="right"| $2,310
 +
|align="right"| $3,150
 +
|align="center"| per flowcell
 +
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year <BR> ** THESE RUNS WILL HAVE LOW PRIORITY
  |-
  |-
-
  | 50nt Run
+
  | 166nt kit <BR> (eg. 80PE or 150SE)
-
  |align="right"| $1,000
+
|align="right"| $3,150
-
  |align="right"| $1,500
+
|align="right"| $3,465
 +
|align="right"| $4,725
 +
|align="center"| per flowcell
 +
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 +
|-
 +
| 316nt kit <BR> (eg. 150PE or 300SE)
 +
|align="right"| $4,725
 +
|align="right"| $5,200
 +
|align="right"| $7,088
 +
|align="center"| per flowcell
 +
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 +
|-
 +
| colspan="6" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
 +
|-
 +
| 70nt Run <BR> (eg. 30PE or 70SE)
 +
|align="right"| $1,155
 +
  |align="right"| $1,270
 +
  |align="right"| $1,732
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
-
  | 300nt Run (PE or SE)
+
  | 300nt Run <BR> (eg. 150PE)
-
  |align="right"| $1,300
+
  |align="right"| $1,365
-
  |align="right"| $1,950
+
  |align="right"| $1,500
 +
|align="right"| $2,050
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
-
  | 500nt Run (PE or SE)
+
  | 500nt Run <BR> (eg. 250PE)
-
  |align="right"| $1,450
+
  |align="right"| $1,575
-
  |align="right"| $2,175
+
|align="right"| $1,732
 +
  |align="right"| $2,362
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
-
|-
+
-
| colspan="5" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
+
  |-
  |-
-
  | colspan="5" align=center |''GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.''
+
  | colspan="6" align=center |<BR>'''MiSeq v3''' sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
  |-
  |-
-
  | 8 Lanes (SE/PE)
+
  | 150nt Run <BR> (eg. 75PE)
-
  |align="right"| $4,835 / $7,135
+
  |align="right"| $1,365
-
  |align="right"| $7,253 / $10,702
+
|align="right"| $1,500
-
  |align="center"| 1 day
+
  |align="right"| $2,050
-
  | 24nt per day. 42nt per seq kit.
+
  |align="center"| per lane
 +
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
-
  | 4 Lanes (SE/PE)
+
  | 600nt Run <BR> (eg. 300PE)
-
  |align="right"| $3,465 / $4,700
+
  |align="right"| $1,890
-
  |align="right"| $5,198 / $7,050
+
  |align="right"| $2,080
-
|align="center"| 1 day
+
  |align="right"| $2,835
-
| 36nt per day. 72nt per seq kit.
+
  |align="center"| per lane
-
|-
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
-
| 2 Lanes (SE/PE)
+
-
  |align="right"| $2,780 / $4,015
+
-
|align="right"| $4,170 / $6,023
+
-
  |align="center"| 1 day
+
-
| 48nt per day. 120nt per seq kit.
+
-
  |-
+
-
| 1 Lane (SE/PE)
+
-
|align="right"| $2,440 / $3,675
+
-
|align="right"| $3,660 / $5,513
+
-
|align="center"| 1 day
+
-
| 72nt per day. 180nt per seq kit.
+
-
|-
+
-
| Add'l Day
+
-
|align="right"| $325
+
-
|align="right"| $488
+
-
|align="center"| 1 day
+
-
| covers service contract and data storage requirements.
+
-
|-
+
-
| Add'l Sequencing Kit
+
-
|align="right"| $1,400
+
-
|align="right"| $2,100
+
-
|align="center"| 1 Kit
+
-
|
+
  |-
  |-
-
  | colspan="5" align=center |<BR>'''Additional Services'''  
+
  | colspan="6" align=center |<BR>'''Additional Services'''  
  |-  
  |-  
-
  | colspan="5" align=center |''These services apply to samples submitted for all platforms.''
+
  | colspan="6" align=center |''These services apply to samples submitted for all platforms.''
  |-  
  |-  
  | Sample Multiplexing
  | Sample Multiplexing
  |align="right"| $50
  |align="right"| $50
 +
|align="right"| $55
  |align="right"| $75
  |align="right"| $75
  |align="center"| per 10 samples
  |align="center"| per 10 samples
-
  | Combined over the whole order. Only applies to samples combined by BMC technicians.
+
  | Combined over the whole order. Only applies to samples combined by BMC technicians.<BR>Waived for libraries created by the BMC.
  |-
  |-
-
  | Add'l Repriming
+
  | Dual Index read (HiSeq only)
  |align="right"| $200
  |align="right"| $200
 +
|align="right"| $220
  |align="right"| $300
  |align="right"| $300
  |align="center"| per flow cell
  |align="center"| per flow cell
-
  | Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
+
  | Uses 6-8nt. Cost is divided by number of lanes requiring dual indexing. Initial index read is included in sequencing cost.  
  |-
  |-
-
  | Quality Control Only
+
  | Quality Control Only -only one QC is included per sequencing lane.
 +
|align="right"| $20
 +
|align="right"| $26
  |align="right"| $30
  |align="right"| $30
-
|align="right"| $45
 
  |align="center"| per sample
  |align="center"| per sample
-
  | Includes RT-PCR and BioAnalyzer
+
  | Includes RT-PCR and Frag.Analyzer
-
  |}
+
  |-
-
<BR>
+
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
 +
|align="right"| $10
 +
|align="right"| $13
 +
|align="right"| $15
 +
|align="center"| per sample
 +
| Includes Fluorometric quantification and Frag.Analyzer
 +
|}
-
<BR>
+
<BR><BR>
-
Prices effective 7.1.2012 <BR><BR>
+
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR>
-
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.
+
-
=== SAMPLE PREPARATION ===
+
=== SAMPLE PREPARATION - STANDARD ===
-
{| border=1 align="left"
+
{| border=1  
-
  ! ILLUMINA Sample Preparation
+
  ! ILLUMINA Sample Preparation - STANDARD
-
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
-
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
+
!width=80| MIT
-
  !width=100| unit
+
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
  !width=80| unit
  ! Notes
  ! Notes
  |-
  |-
-
  | colspan="5" | '''STARTING WITH DNA'''  
+
  | colspan="6" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 +
|-
 +
| NEOPREP DNA
 +
|align="right"| $70
 +
|align="right"| $77
 +
|align="right"| $105
 +
|align="center"| per sample
 +
| Includes adapter ligation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Insert sizes above 250bp.
  |-
  |-
  | Full Service Sheared DNA (SPRIWORKS)
  | Full Service Sheared DNA (SPRIWORKS)
-
  |align="right"| $150
+
  |align="right"| $130
-
  |align="right"| $225
+
  |align="right"| $143
 +
|align="right"| $195
  |align="center"| per sample
  |align="center"| per sample
  | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
  | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
  |-
  |-
  | ''SPRI-TE use only''
  | ''SPRI-TE use only''
-
  |align="right"| $100
+
  |align="right"| $85
 +
|align="right"| $95
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per sample
  |align="center"| per sample
-
  | User must provide all primers. We do not QC the samples. Samples will be returned for amplification.
+
  | User must provide all primers. Samples will be returned for amplification.
  |-
  |-
-
  | Full Service Intact Genomic DNA (NEXTERA)
+
  | Intact Genomic DNA (NEXTERA) - 50ng input
  |align="right"| $150
  |align="right"| $150
 +
|align="right"| $165
  |align="right"| $225
  |align="right"| $225
  |align="center"| per sample
  |align="center"| per sample
-
  | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+
  | Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
  |-
  |-
-
  | colspan="5" | '''STARTING WITH RNA'''
+
  | Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input
 +
|align="right"| $100
 +
|align="right"| $110
 +
|align="right"| $150
 +
|align="center"| per sample
 +
| Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
  |-
  |-
-
  | Illumina TruSeq
+
| colspan="6" | '''STARTING WITH RNA'''<BR>Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
-
  |align="right"| $300
+
|-
-
  |align="right"| $450
+
  | Illumina NEOPREP
 +
|align="right"| $100
 +
  |align="right"| $110
 +
  |align="right"| $150
  |align="center"| per sample
  |align="center"| per sample
-
  | Preformed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+
  | Performed using Neoprep system. <br> Requires polyA+ total RNA as input. No other inputs allowed. <BR>Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer)
  |-
  |-
-
  |''+strand specific''
+
  | Illumina TruSeq (stranded or non)
-
  |align="right"| $30
+
  |align="right"| $275
-
  |align="right"| $45
+
  |align="right"| $302.5
 +
|align="right"| $412.5
  |align="center"| per sample
  |align="center"| per sample
-
  | Requires 1ug of RNA. Uses the dUTP method.
+
  | Performed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Strand specifc by dUTP method
  |-
  |-
  | Epicenter Ribozero<br>''no polyA pulldown''
  | Epicenter Ribozero<br>''no polyA pulldown''
  |align="right"| $350
  |align="right"| $350
 +
|align="right"| $385
  |align="right"| $525
  |align="right"| $525
  |align="center"| per sample
  |align="center"| per sample
Line 171: Line 204:
  | Low Input (Clontech)
  | Low Input (Clontech)
  |align="right"| $375
  |align="right"| $375
-
  |align="right"| $575
+
  |align="right"| $412.5
 +
|align="right"| $562.5
  |align="center"| per sample
  |align="center"| per sample
  | Can work on single cells
  | Can work on single cells
  |-
  |-
  | Low Input (NuGen)
  | Low Input (NuGen)
-
  |align="right"| $400
+
  |align="right"| $430
-
  |align="right"| $600
+
  |align="right"| $473
 +
|align="right"| $645
  |align="center"| per sample
  |align="center"| per sample
  | Can work on degraded RNA.
  | Can work on degraded RNA.
  |-
  |-
  | Tight Size Cut
  | Tight Size Cut
-
  |align="right"| $75
+
  |align="right"| $85
-
  |align="right"| $125
+
  |align="right"| $94
 +
|align="right"| $130
  |align="center"| per pool
  |align="center"| per pool
  | +/- 40bp using PippinPrep
  | +/- 40bp using PippinPrep
  |-
  |-
-
  | colspan="5" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
+
| Small RNA
 +
| align="right"|$275
 +
| align="right"|$303
 +
| align="right"|$413
 +
| align="center"|per sample
 +
| Uses NEB small RNA kit
 +
|-
 +
  | colspan="6" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
  |-
  |-
  | Full Service ChIP-seq
  | Full Service ChIP-seq
  |align="right"| $210
  |align="right"| $210
 +
|align="right"| $231
  |align="right"| $315
  |align="right"| $315
  |align="center"| per sample
  |align="center"| per sample
-
  | Preformed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
+
  | Performed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
  |-
  |-
  | ''IP-Star Only''
  | ''IP-Star Only''
-
  |align="right"| $70
+
  |align="right"| $300
-
  |align="right"| $105
+
  |align="right"| $330
-
  |align="center"| per sample
+
|align="right"| $450
-
  | Preformed using IP-Star
+
  |align="center"| per batch (1-8 samples)
 +
  | Performed using IP-Star
  |-
  |-
-
  | ''qPCR of ChIP''<br> ''in development''
+
  | Covaris Sonicator
-
  |align="right"| TBA
+
  |align="right"| $15
-
  |align="right"| TBA
+
  |align="right"| $20
-
  |align="center"| per sample
+
|align="right"| NA
-
  |Tests 8 IPs against 5 genomic loci in duplicate. User provides oligos.
+
  |align="center"| per hour
 +
  | Training Required
  |-
  |-
|}
|}
-
<BR>
+
 
-
<BR>
+
-
<BR>
+
.
.
-
<BR>
+
=== SAMPLE PREPARATION - HIGH THROUGHPUT ===
-
<BR>
+
 
-
<BR>
+
{| border=1
-
<BR>
+
! ILLUMINA Sample Preparation - HIGH-THROUGHPUT
-
<BR>
+
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 +
!width=80| MIT
 +
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
!width=80| Batch size
 +
!width=160| INPUT REQUIREMENT
 +
! Notes
 +
|-
 +
| colspan="7" | '''LIBRARY PREP''' <BR> Repreps will NOT be attempted on failed samples without charge. Submission must be coordinated with BMC staff.
 +
|-
 +
| AUTOMATED NEXTERA-XT - 1ng input
 +
|align="right"| $1000
 +
|align="right"| $1100
 +
|align="right"| $1500
 +
|align="center"| 16 samples <BR>(2 column)
 +
|>7ul at 0.2ng/ul in Axygen plate
 +
| Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). May have 10% failure rate.
 +
|-
 +
| 16S Amplicon
 +
|align="right"| $1,800
 +
|align="right"| $1,980
 +
|align="right"| $2,700
 +
|align="center"|48 samples <BR>(6 columns)
 +
| >5ul, must amplify with Ct <20 by qPCR.
 +
| Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
| Other Amplicon
 +
|align="right"| $1,440
 +
|align="right"| $1,584
 +
|align="right"| $2,160
 +
|align="center"|48 samples <BR>(6 columns)
 +
| >5ul, must amplify with Ct <20 by qPCR.
 +
| Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). <BR> Samples *must* be submitted amplified with appropriate linkers.
 +
|-
 +
| 3'DGE
 +
|align="right"| $1,200
 +
|align="right"| $1,320
 +
|align="right"| $1,800
 +
|align="center"|24 samples <BR>(2 rows)
 +
|5ul of RIN7+ polyA+ RNA.
 +
|Includes cDNA generation, amplification, pooling and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
| colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in ideal input format will be subject to these additional charges.
 +
|-
 +
|Setup
 +
|align="right"| $50
 +
|align="right"| $55
 +
|align="right"| $75
 +
|align="center"|48 samples
 +
|
 +
|
 +
|-
 +
|Cleanup
 +
|align="right"| $5
 +
|align="right"| $5.5
 +
|align="right"| $7.5
 +
|align="center"|per sample
 +
|
 +
|SPRI cleanup of samples to remove impurities.
 +
|-
 +
|Quantification
 +
|align="right"| $5
 +
|align="right"| $5.5
 +
|align="right"| $7.5
 +
|align="center"|per sample
 +
|
 +
|Quantification of samples (FA) prior to arraying.
 +
|-
 +
|}
== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ==
== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ==
Line 232: Line 343:
  |-
  |-
  | Eukaryote (3' or Exon: >20ng)
  | Eukaryote (3' or Exon: >20ng)
-
  |align="right"| $270
+
  |align="right"| $200
-
  |align="right"| $360
+
  |align="right"| $250
-
  |align="right"| $540
+
  |align="right"| $325
  |align="center"| per sample
  |align="center"| per sample
-
  | Using NuGEN sample labeling kit.
+
  | Using AFFYMETRIX sample labeling kit. For NuGEN reagents, please refer to 2015 pricing.
  |-
  |-
  | Eukaryote (<20ng)
  | Eukaryote (<20ng)
-
  |align="right"| $370
+
  |align="right"| $400
-
  |align="right"| $460
+
  |align="right"| $500
-
  |align="right"| $690
+
  |align="right"| $750
  |align="center"| per sample
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
  | Using NuGEN sample labeling kit.
Line 248: Line 359:
  |align="right"| $365
  |align="right"| $365
  |align="right"| $455
  |align="right"| $455
-
  |align="right"| $680
+
  |align="right"| $683
  |align="center"| per sample
  |align="center"| per sample
-
  | Using Ambion sample labeling kit
+
  | Using Ambion sample labeling kit.   
-
|-
+
-
| Fluidics Station and Scan
+
-
|align="right"| $140
+
-
|align="right"| $165
+
-
|align="right"| N/A
+
-
|align="center"| per array
+
-
| Users must be trained and coordinate with Manlin.   
+
  |-
  |-
  | colspan="6" | '''AGILENT PROCESSING'''
  | colspan="6" | '''AGILENT PROCESSING'''
  |-
  |-
-
  | Expression - ONE COLOR - Eukaryote
+
  | colspan="6" align=center |Agilent array services are being phased out in FY2016. Requests that must be processed on Agilent will be priced per experiment.
-
|align="right"| $180
+
-
|align="right"| $240
+
-
|align="right"| $360
+
-
|align="center"| per array
+
-
|
+
-
|-
+
-
| Expression - TWO COLOR - Eukaryote
+
-
|align="right"| $250
+
-
|align="right"| $300
+
-
|align="right"| $450
+
-
|align="center"| per array
+
-
| Includes both Cy5 and Cy3
+
-
|-
+
-
| miRNA - ONE COLOR
+
-
|align="right"| $230
+
-
|align="right"| $300
+
-
|align="right"| $450
+
-
|align="center"| per array
+
-
|
+
-
|-
+
-
| Prokaryote - ONE COLOR
+
-
|align="right"| $230
+
-
|align="right"| $300
+
-
|align="right"| $450
+
-
|align="center"| per array
+
-
|
+
  |-  
  |-  
  | Scanning Only
  | Scanning Only
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  |-  
  |-  
|}
|}
-
prices effective 7.1.2012
+
prices effective 7.1.2015
<br>
<br>
Line 310: Line 388:
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
{| border=1
{| border=1
-
  ! AGILENT BIOANALYZER
+
  ! AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
Line 316: Line 394:
  ! Notes
  ! Notes
  |-
  |-
-
  | High Sensitivity DNA or Nano RNA
+
  | RNA / DNA on Advanced Analytical
-
  |align="right"| $10
+
  |align="right"| $8
-
  |align="right"| $25
+
  |align="right"| $12
  |align="center"| per sample
  |align="center"| per sample
  |  
  |  
  |-
  |-
-
  | All other DNA and RNA samples
+
| AA - Preloaded plate
 +
|align="right"| $30
 +
|align="right"| N/A
 +
|align="center"| per row (12)
 +
| 2ul per sample in each well using plates from BMC
 +
|-
 +
  | All DNA & RNA on BIOANALYZER
  |align="right"| $15
  |align="right"| $15
  |align="right"| $25
  |align="right"| $25
Line 333: Line 417:
  |align="center"| per sample
  |align="center"| per sample
  | detection of proteins at < 30ng/ul.   
  | detection of proteins at < 30ng/ul.   
-
|-
 
-
| Flow Cytometry
 
-
|align="right"| by request
 
-
|align="right"| NA
 
-
|align="center"| per sample
 
-
 
  |-
  |-
|}
|}
-
prices effective 7.1.2012
+
prices effective 7.1.2015
<br>.
<br>.
Line 353: Line 431:
  ! SIGN UP
  ! SIGN UP
  ! Notes
  ! Notes
-
|-
 
-
| colspan="6" align="center"| '''FLUIDIGM'''
 
-
|-
 
-
| Fluidigm BioMark
 
-
|align="right"| $150
 
-
|align="right"| $225
 
-
|align="center"| per use
 
-
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
 
-
| Contact [[BioMicroCenter:People|Shmulik]]
 
-
|-
 
-
| 48.48 Dynamic Array Chip
 
-
|align="right"| $260
 
-
|align="right"| N/A
 
-
|align="center"| per cassette
 
-
| Cassettes are available in the BMC.
 
-
| Contact [[BioMicroCenter:People|Shmulik]]
 
-
|-
 
-
| 96.96 Dynamic Array Chip
 
-
|align="right"| $760
 
-
|align="right"| N/A
 
-
|align="center"| per cassette
 
-
| Cassettes are available in the BMC.
 
-
| Contact [[BioMicroCenter:People|Shmulik]]
 
-
|-
 
-
| colspan="6" align="center"| '''RT-PCR'''
 
  |-
  |-
  | Roche LightCycler 480
  | Roche LightCycler 480
  |align="right"| $10
  |align="right"| $10
-
  |align="right"| NA
+
  |align="right"| N/A
  |align="center"| per plate
  |align="center"| per plate
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
-
  | Contact [[BioMicroCenter:People|Michael]] for training
+
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training
-
|-
+
-
| MJ Opticon II
+
-
|align="right"| $10
+
-
|align="right"| $15
+
-
|align="center"| per plate
+
-
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
+
-
| Contact [[BioMicroCenter:People|Manlin]] for training
+
  |-
  |-
  | SYBR Green (KAPA/Roche)
  | SYBR Green (KAPA/Roche)
-
  |align="right"| $34
+
  |align="right"| $36
-
  |align="right"| NA
+
  |align="right"| N/A
  |align="center"| per ml
  |align="center"| per ml
  |
  |
  | Available for MIT only  
  | Available for MIT only  
  |-
  |-
-
  | 96-well RTPCR PLATE
+
  | 96-well PLATES
  |align="right"| $6
  |align="right"| $6
-
  |align="right"| NA
+
  |align="right"| N/A
  |align="center"| per plate
  |align="center"| per plate
  |
  |
Line 408: Line 454:
  |-
  |-
|}
|}
-
prices effective 7.1.2012
 
.
.
<br>
<br>
Line 436: Line 481:
  |align="center"| per session
  |align="center"| per session
  | TBA
  | TBA
-
  | Contact [[BioMicroCenter:People|Shmulik]] for training.
+
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training.
  |-
  |-
| colspan="6" align="center"| '''PLATE READER'''
| colspan="6" align="center"| '''PLATE READER'''
  |-
  |-
  | Varioskan
  | Varioskan
-
  |align="right"| $10
+
  |align="right"| $18
-
  |align="right"| $15
+
  |align="right"| $27
-
  |align="center"| per session
+
  |align="center"| per hour
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
-
  | Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Shmulik]] for training  
+
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training  
  |-
  |-
  | colspan="6" align="center"| '''ROBOTICS'''
  | colspan="6" align="center"| '''ROBOTICS'''
Line 451: Line 496:
  | Tecan EVO 150
  | Tecan EVO 150
  |align="right"| $50
  |align="right"| $50
-
  |align="right"| $150
+
  |align="right"| $55
-
  |align="center"| per 2h block - based on scheduled time.
+
  |align="center"| per 1h block - based on scheduled time.
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  |  
  |  
Line 471: Line 516:
  |-
  |-
  | Training
  | Training
-
  |align="right"| $100
+
  |align="right"| $50
-
  |align="right"| $150
+
  |align="right"| $55
  |align="center"| per session
  |align="center"| per session
  | Contact [[BioMicroCenter:People|Stuart]]
  | Contact [[BioMicroCenter:People|Stuart]]
Line 487: Line 532:
  |-
  |-
|}
|}
-
prices effective 7.1.2012
 
<br><br>
<br><br>
Line 495: Line 539:
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| Other MIT / [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| Other MIT / [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
-
  !width=100| unit
+
  !width=200| unit
  ! Notes
  ! Notes
  |-
  |-
  | Informatics Support
  | Informatics Support
-
  | align="right"| $65
+
  | align="right"| $75
-
  | align="right"| $85 / $120
+
  | align="right"| $100 / $130
  | align="right"| per hour
  | align="right"| per hour
  | Limited support for non-MIT users
  | Limited support for non-MIT users
 +
|-
 +
| Informatics Share
 +
| align="right"| $1,000
 +
| align="right"| N/A
 +
| align="right"| per 0.1FTE
 +
| MIT only. Priority access to informatics.
  |-
  |-
  | Data storage (1 year)
  | Data storage (1 year)
-
  |align="right"| $400
+
  |align="right"| $280
-
  |align="right"| N/A
+
  |align="right"| $400 / N/A
  |align="center"| per TB
  |align="center"| per TB
-
  | (Limited space available)
+
  |  
  |-
  |-
-
  | Spotfire License (1 year)
+
  | Archival Storage (1 year - read only. Unmutable)
-
  |align="right"| ''Contact Stephen Goldman''
+
  |align="right"| $140
-
  |align="right"| N/A
+
  |align="right"| $200 / N/A
-
  |align="center"| per user
+
  |align="center"| per TB
-
  | (Limited seats available)
+
|
 +
|-
 +
| Tape Archive / Recovery
 +
|align="right"| $60
 +
|align="right"| $70
 +
|align="center"| per tape
 +
  |  
  |-
  |-
|}
|}
 +
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]]<BR>
 +
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]]<BR>
 +
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]]<BR>
 +
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]]<BR>
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]<BR>
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]<BR>
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]<BR>
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]<BR>
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

Current revision

Image:BioMicroCenter-header6.jpg

Contents

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the HiSeq.

ILLUMINA SEQUENCING CORE LAB MIT Non-MIT unit Notes

HiSEQ 2000 sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
Single End (40nt) $1,140 $1,254 $1,710 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Paired End (40+40nt) $1,710 $1,881 $2,565 per lane
Add'l 40nt $330 $363 $495 per lane

NextSeq sequencing (minimum 250m reads/lane. maximum ~500m reads/lane)
91nt kit
(eg. 40PE or 50SE)
$2,100 $2,310 $3,150 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
** THESE RUNS WILL HAVE LOW PRIORITY
166nt kit
(eg. 80PE or 150SE)
$3,150 $3,465 $4,725 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
316nt kit
(eg. 150PE or 300SE)
$4,725 $5,200 $7,088 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

MiSeq v2 sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
70nt Run
(eg. 30PE or 70SE)
$1,155 $1,270 $1,732 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
300nt Run
(eg. 150PE)
$1,365 $1,500 $2,050 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
500nt Run
(eg. 250PE)
$1,575 $1,732 $2,362 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

MiSeq v3 sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
150nt Run
(eg. 75PE)
$1,365 $1,500 $2,050 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
600nt Run
(eg. 300PE)
$1,890 $2,080 $2,835 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

Additional Services
These services apply to samples submitted for all platforms.
Sample Multiplexing $50 $55 $75 per 10 samples Combined over the whole order. Only applies to samples combined by BMC technicians.
Waived for libraries created by the BMC.
Dual Index read (HiSeq only) $200 $220 $300 per flow cell Uses 6-8nt. Cost is divided by number of lanes requiring dual indexing. Initial index read is included in sequencing cost.
Quality Control Only -only one QC is included per sequencing lane. $20 $26 $30 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $10 $13 $15 per sample Includes Fluorometric quantification and Frag.Analyzer



Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SAMPLE PREPARATION - STANDARD

ILLUMINA Sample Preparation - STANDARD CORE LAB MIT Non-MIT unit Notes
STANDARD THROUGHPUT DNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
NEOPREP DNA $70 $77 $105 per sample Includes adapter ligation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Insert sizes above 250bp.
Full Service Sheared DNA (SPRIWORKS) $130 $143 $195 per sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only $85 $95 N/A per sample User must provide all primers. Samples will be returned for amplification.
Intact Genomic DNA (NEXTERA) - 50ng input $150 $165 $225 per sample Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input $100 $110 $150 per sample Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
STARTING WITH RNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
Illumina NEOPREP $100 $110 $150 per sample Performed using Neoprep system.
Requires polyA+ total RNA as input. No other inputs allowed.
Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer)
Illumina TruSeq (stranded or non) $275 $302.5 $412.5 per sample Performed using TruSeq AND SPRIworks system.
Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Strand specifc by dUTP method
Epicenter Ribozero
no polyA pulldown
$350 $385 $525 per sample Requires 1ug of RNA. Based on Illumina Truseq chemistry
Low Input (Clontech) $375 $412.5 $562.5 per sample Can work on single cells
Low Input (NuGen) $430 $473 $645 per sample Can work on degraded RNA.
Tight Size Cut $85 $94 $130 per pool +/- 40bp using PippinPrep
Small RNA $275 $303 $413 per sample Uses NEB small RNA kit
STARTING WITH CHROMATIN Note: User must supply antibodies.
Full Service ChIP-seq $210 $231 $315 per sample Performed using IP-Star AND SPRIworks system.
Includes ChIP, library contruction and barcoding.
IP-Star Only $300 $330 $450 per batch (1-8 samples) Performed using IP-Star
Covaris Sonicator $15 $20 NA per hour Training Required

.

SAMPLE PREPARATION - HIGH THROUGHPUT

ILLUMINA Sample Preparation - HIGH-THROUGHPUT CORE LAB MIT Non-MIT Batch size INPUT REQUIREMENT Notes
LIBRARY PREP
Repreps will NOT be attempted on failed samples without charge. Submission must be coordinated with BMC staff.
AUTOMATED NEXTERA-XT - 1ng input $1000 $1100 $1500 16 samples
(2 column)
>7ul at 0.2ng/ul in Axygen plate Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). May have 10% failure rate.
16S Amplicon $1,800 $1,980 $2,700 48 samples
(6 columns)
>5ul, must amplify with Ct <20 by qPCR. Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
Other Amplicon $1,440 $1,584 $2,160 48 samples
(6 columns)
>5ul, must amplify with Ct <20 by qPCR. Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
Samples *must* be submitted amplified with appropriate linkers.
3'DGE $1,200 $1,320 $1,800 24 samples
(2 rows)
5ul of RIN7+ polyA+ RNA. Includes cDNA generation, amplification, pooling and quality control (RT-PCR and BioAnalyzer).
ADDITIONAL FEES
Samples not in ideal input format will be subject to these additional charges.
Setup $50 $55 $75 48 samples
Cleanup $5 $5.5 $7.5 per sample SPRI cleanup of samples to remove impurities.
Quantification $5 $5.5 $7.5 per sample Quantification of samples (FA) prior to arraying.

MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS CORE LAB MIT Non-MIT unit Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng) $200 $250 $325 per sample Using AFFYMETRIX sample labeling kit. For NuGEN reagents, please refer to 2015 pricing.
Eukaryote (<20ng) $400 $500 $750 per sample Using NuGEN sample labeling kit.
Prokaryote $365 $455 $683 per sample Using Ambion sample labeling kit.
AGILENT PROCESSING
Agilent array services are being phased out in FY2016. Requests that must be processed on Agilent will be priced per experiment.
Scanning Only $50 $50 N/A per slide Price per glass slide.

prices effective 7.1.2015

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices
  • available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER CORE LAB / MIT Non-MIT unit Notes
RNA / DNA on Advanced Analytical $8 $12 per sample
AA - Preloaded plate $30 N/A per row (12) 2ul per sample in each well using plates from BMC
All DNA & RNA on BIOANALYZER $15 $25 per sample
High Sensitivity protein $40 $60 per sample detection of proteins at < 30ng/ul.

prices effective 7.1.2015
.

REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
Roche LightCycler 480 $10 N/A per plate CALENDER *available for CORE only Contact Jon Penterman for training
SYBR Green (KAPA/Roche) $36 N/A per ml Available for MIT only
96-well PLATES $6 N/A per plate Available for MIT only

.

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
PIPPIN PREP
Gel Isolation $50 $75 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Jon Penterman for training.
PLATE READER
Varioskan $18 $27 per hour CALENDER Contact Jon Penterman for training
ROBOTICS
Tecan EVO 150 $50 $55 per 1h block - based on scheduled time. CALENDER
non-filter tips $6.00 $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $50 $55 per session Contact Stuart
NANODROP
Nanodrop -- -- Walk up service * no charge



COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES CORE LAB Other MIT / Non-MIT unit Notes
Informatics Support $75 $100 / $130 per hour Limited support for non-MIT users
Informatics Share $1,000 N/A per 0.1FTE MIT only. Priority access to informatics.
Data storage (1 year) $280 $400 / N/A per TB
Archival Storage (1 year - read only. Unmutable) $140 $200 / N/A per TB
Tape Archive / Recovery $60 $70 per tape

FY2017 Pricing
FY2016 Pricing
FY2015 Pricing
FY2014 Pricing
FY2013 Pricing
FY2012 Pricing
FY2011 Pricing

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