BioMicroCenter:Pricing

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(COMPUTATIONAL SERVICES)
(AGILENT BIOANALYZER)
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Line 15: Line 15:
  |-
  |-
  | Single End (40nt)
  | Single End (40nt)
-
  |align="right"| $1,170
+
  |align="right"| $1,060
-
  |align="right"| $1,755
+
  |align="right"| $1,590
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 yrs.
+
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
  |-
  |-
  | Paired End (40+40nt)
  | Paired End (40+40nt)
-
  |align="right"| $1,850
+
  |align="right"| $1,575
-
  |align="right"| $2,775
+
  |align="right"| $2,363
  |align="center"| per lane
  |align="center"| per lane
  |  
  |  
  |-
  |-
  | Add'l 40nt
  | Add'l 40nt
-
  |align="right"| $370
+
  |align="right"| $300
-
  |align="right"| $555
+
  |align="right"| $450
  |align="center"| per lane
  |align="center"| per lane
  |   
  |   
  |-
  |-
-
  | colspan="5" align=center |<BR>'''MiSeq''' sequencing (minimum 2m reads/lane. Average ~5m reads/lane)
+
  | colspan="5" align=center |<BR>'''MiSeq''' sequencing (minimum 8m reads/lane. Average ~15m reads/lane)
  |-
  |-
-
  | 50nt Run
+
  | 70nt Run
-
  |align="right"| $1,000
+
  |align="right"| $1,100
-
  |align="right"| $1,500
+
  |align="right"| $1,650
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
+
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
  | 300nt Run (PE or SE)
  | 300nt Run (PE or SE)
Line 44: Line 44:
  |align="right"| $1,950
  |align="right"| $1,950
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
+
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
  | 500nt Run (PE or SE)
  | 500nt Run (PE or SE)
-
  |align="right"| $1,450
+
  |align="right"| $1,500
-
  |align="right"| $2,175
+
  |align="right"| $2,250
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
+
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
-
  |-  
+
  |-
  | colspan="5" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
  | colspan="5" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
  |-
  |-
Line 57: Line 57:
  |-
  |-
  | 8 Lanes (SE/PE)
  | 8 Lanes (SE/PE)
-
  |align="right"| $4,835 / $7,135
+
  |align="right"| $5,200 / $7,550
-
  |align="right"| $7,253 / $10,702
+
  |align="right"| $7,800 / $11,325
  |align="center"| 1 day
  |align="center"| 1 day
-
  | 24nt per day. 42nt per seq kit.
+
  | 24nt per day. 45nt per seq kit.
  |-
  |-
  | 4 Lanes (SE/PE)
  | 4 Lanes (SE/PE)
-
  |align="right"| $3,465 / $4,700
+
  |align="right"| $3,800 / $5,075
-
  |align="right"| $5,198 / $7,050
+
  |align="right"| $5,700 / $7,613
  |align="center"| 1 day
  |align="center"| 1 day
-
  | 36nt per day. 72nt per seq kit.
+
  | 36nt per day. 66nt per seq kit.
  |-
  |-
  | 2 Lanes (SE/PE)
  | 2 Lanes (SE/PE)
-
  |align="right"| $2,780 / $4,015
+
  |align="right"| $3,100 / $4,375
-
  |align="right"| $4,170 / $6,023
+
  |align="right"| $4,650 / $6,563
  |align="center"| 1 day
  |align="center"| 1 day
-
  | 48nt per day. 120nt per seq kit.
+
  | 48nt per day. 102nt per seq kit.
  |-
  |-
  | 1 Lane (SE/PE)
  | 1 Lane (SE/PE)
-
  |align="right"| $2,440 / $3,675
+
  |align="right"| $2,750 / NA
-
  |align="right"| $3,660 / $5,513
+
  |align="right"| $4,125 / NA
  |align="center"| 1 day
  |align="center"| 1 day
-
  | 72nt per day. 180nt per seq kit.
+
  | 72nt per day. 140nt per seq kit.
  |-
  |-
  | Add'l Day
  | Add'l Day
-
  |align="right"| $325
+
  |align="right"| $600
-
  |align="right"| $488
+
  |align="right"| $900
  |align="center"| 1 day
  |align="center"| 1 day
  | covers service contract and data storage requirements.
  | covers service contract and data storage requirements.
  |-
  |-
  | Add'l Sequencing Kit
  | Add'l Sequencing Kit
-
  |align="right"| $1,400
+
  |align="right"| $1,450
-
  |align="right"| $2,100
+
  |align="right"| $2,175
  |align="center"| 1 Kit
  |align="center"| 1 Kit
  |  
  |  
Line 109: Line 109:
  |-
  |-
  | Quality Control Only
  | Quality Control Only
-
  |align="right"| $30
+
  |align="right"| $25
-
  |align="right"| $45
+
  |align="right"| $38
  |align="center"| per sample
  |align="center"| per sample
  | Includes RT-PCR and BioAnalyzer
  | Includes RT-PCR and BioAnalyzer
Line 117: Line 117:
<BR>
<BR>
-
Prices effective 7.1.2012 <BR><BR>
+
Prices effective 7.1.2013 <BR><BR>
-
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.
+
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Hi-Seq. The BioMicro Center reserves the right to reject samples for sample preparations.
=== SAMPLE PREPARATION ===
=== SAMPLE PREPARATION ===
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  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per sample
  |align="center"| per sample
-
  | User must provide all primers. We do not QC the samples. Samples will be returned for amplification.
+
  | User must provide all primers. Samples will be returned for amplification.
  |-
  |-
  | Full Service Intact Genomic DNA (NEXTERA)
  | Full Service Intact Genomic DNA (NEXTERA)
Line 170: Line 170:
  |-
  |-
  | Low Input (Clontech)
  | Low Input (Clontech)
-
  |align="right"| $375
+
  |align="right"| $400
-
  |align="right"| $575
+
  |align="right"| $600
  |align="center"| per sample
  |align="center"| per sample
  | Can work on single cells
  | Can work on single cells
  |-
  |-
  | Low Input (NuGen)
  | Low Input (NuGen)
-
  |align="right"| $400
+
  |align="right"| $430
-
  |align="right"| $600
+
  |align="right"| $645
  |align="center"| per sample
  |align="center"| per sample
  | Can work on degraded RNA.
  | Can work on degraded RNA.
  |-
  |-
  | Tight Size Cut
  | Tight Size Cut
-
  |align="right"| $75
+
  |align="right"| $85
-
  |align="right"| $125
+
  |align="right"| $130
  |align="center"| per pool
  |align="center"| per pool
  | +/- 40bp using PippinPrep
  | +/- 40bp using PippinPrep
 +
|-
 +
| Small RNA
 +
| align="right"|$275
 +
| align="right"|$413
 +
| align="center"|per sample
 +
| Uses NEB small RNA kit
  |-
  |-
  | colspan="5" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
  | colspan="5" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
Line 196: Line 202:
  |-
  |-
  | ''IP-Star Only''
  | ''IP-Star Only''
-
  |align="right"| $70
+
  |align="right"| $300
-
  |align="right"| $105
+
  |align="right"| $450
-
  |align="center"| per sample
+
  |align="center"| per batch (1-8 samples)
  | Preformed using IP-Star
  | Preformed using IP-Star
-
|-
 
-
| ''qPCR of ChIP''<br> ''in development''
 
-
|align="right"| TBA
 
-
|align="right"| TBA
 
-
|align="center"| per sample
 
-
|Tests 8 IPs against 5 genomic loci in duplicate. User provides oligos.
 
  |-
  |-
|}
|}
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  |-
  |-
  | Eukaryote (3' or Exon: >20ng)
  | Eukaryote (3' or Exon: >20ng)
-
  |align="right"| $270
+
  |align="right"| $300
-
  |align="right"| $360
+
  |align="right"| $380
-
  |align="right"| $540
+
  |align="right"| $570
  |align="center"| per sample
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
  | Using NuGEN sample labeling kit.
  |-
  |-
  | Eukaryote (<20ng)
  | Eukaryote (<20ng)
-
  |align="right"| $370
+
  |align="right"| $400
-
  |align="right"| $460
+
  |align="right"| $500
-
  |align="right"| $690
+
  |align="right"| $750
  |align="center"| per sample
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
  | Using NuGEN sample labeling kit.
Line 248: Line 248:
  |align="right"| $365
  |align="right"| $365
  |align="right"| $455
  |align="right"| $455
-
  |align="right"| $680
+
  |align="right"| $683
  |align="center"| per sample
  |align="center"| per sample
-
  | Using Ambion sample labeling kit
+
  | Using Ambion sample labeling kit.   
-
|-
+
-
| Fluidics Station and Scan
+
-
|align="right"| $140
+
-
|align="right"| $165
+
-
|align="right"| N/A
+
-
|align="center"| per array
+
-
| Users must be trained and coordinate with Manlin.   
+
  |-
  |-
  | colspan="6" | '''AGILENT PROCESSING'''
  | colspan="6" | '''AGILENT PROCESSING'''
  |-
  |-
  | Expression - ONE COLOR - Eukaryote
  | Expression - ONE COLOR - Eukaryote
-
  |align="right"| $180
+
  |align="right"| $200
-
  |align="right"| $240
+
  |align="right"| $250
-
  |align="right"| $360
+
  |align="right"| $375
  |align="center"| per array
  |align="center"| per array
  |  
  |  
  |-
  |-
  | Expression - TWO COLOR - Eukaryote
  | Expression - TWO COLOR - Eukaryote
-
  |align="right"| $250
+
  |align="right"| $290
-
  |align="right"| $300
+
  |align="right"| $340
-
  |align="right"| $450
+
  |align="right"| $510
  |align="center"| per array
  |align="center"| per array
  | Includes both Cy5 and Cy3
  | Includes both Cy5 and Cy3
  |-
  |-
  | miRNA - ONE COLOR
  | miRNA - ONE COLOR
-
  |align="right"| $230
+
  |align="right"| $260
-
  |align="right"| $300
+
  |align="right"| $320
-
  |align="right"| $450
+
  |align="right"| $480
  |align="center"| per array
  |align="center"| per array
  |  
  |  
  |-
  |-
  | Prokaryote - ONE COLOR
  | Prokaryote - ONE COLOR
-
  |align="right"| $230
+
  |align="right"| $200
-
  |align="right"| $300
+
  |align="right"| $260
-
  |align="right"| $450
+
  |align="right"| $390
  |align="center"| per array
  |align="center"| per array
  |  
  |  
Line 297: Line 290:
  |-  
  |-  
|}
|}
-
prices effective 7.1.2012
+
prices effective 7.1.2013
<br>
<br>
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  $400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
  $400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
  Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
  Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
-
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.
+
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.  
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
{| border=1
{| border=1
-
  ! AGILENT BIOANALYZER
+
  ! AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
Line 316: Line 309:
  ! Notes
  ! Notes
  |-
  |-
-
  | High Sensitivity DNA or Nano RNA
+
  | RNA / DNA on Advanced Analytical
-
  |align="right"| $10
+
  |align="right"| $8
-
  |align="right"| $25
+
  |align="right"| $12
  |align="center"| per sample
  |align="center"| per sample
  |  
  |  
  |-
  |-
-
  | All other DNA and RNA samples
+
| AA - Preloaded plate
 +
|align="right"| $30
 +
|align="right"|
 +
|align="center"| per row (12)
 +
| 2ul per sample in each well using plates from BMC
 +
|-
 +
  | All DNA & RNA on BIOANALYZER
  |align="right"| $15
  |align="right"| $15
  |align="right"| $25
  |align="right"| $25
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  |align="center"| per sample
  |align="center"| per sample
  | detection of proteins at < 30ng/ul.   
  | detection of proteins at < 30ng/ul.   
-
|-
 
-
| Flow Cytometry
 
-
|align="right"| by request
 
-
|align="right"| NA
 
-
|align="center"| per sample
 
-
 
  |-
  |-
|}
|}
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  |-
  |-
  | 48.48 Dynamic Array Chip
  | 48.48 Dynamic Array Chip
-
  |align="right"| $260
+
  |align="right"| $250
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per cassette
  |align="center"| per cassette
  | Cassettes are available in the BMC.
  | Cassettes are available in the BMC.
-
  | Contact [[BioMicroCenter:People|Shmulik]]
+
  |
  |-
  |-
  | 96.96 Dynamic Array Chip
  | 96.96 Dynamic Array Chip
-
  |align="right"| $760
+
  |align="right"| $700
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per cassette
  |align="center"| per cassette
  | Cassettes are available in the BMC.
  | Cassettes are available in the BMC.
-
  | Contact [[BioMicroCenter:People|Shmulik]]
+
  |
  |-
  |-
-
  | colspan="6" align="center"| '''RT-PCR'''
+
  | WAFERGEN SMART CHIP
 +
|align="right"| $150
 +
|align="right"| N/A
 +
|align="center"| per chip
 +
| Chips available from BMC
 +
| Contact [[BioMicroCenter:People|Michael]]
 +
|-
 +
| WAFERGEN TRAINING
 +
|align="right"| $100
 +
|align="right"| N/A
 +
|align="center"| per chip
 +
| Mandatory
 +
| Contact [[BioMicroCenter:People|Michael]]
 +
|- | colspan="6" align="center"| '''RT-PCR'''
  |-
  |-
  | Roche LightCycler 480
  | Roche LightCycler 480
Line 385: Line 391:
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
  | Contact [[BioMicroCenter:People|Michael]] for training
  | Contact [[BioMicroCenter:People|Michael]] for training
-
|-
 
-
| MJ Opticon II
 
-
|align="right"| $10
 
-
|align="right"| $15
 
-
|align="center"| per plate
 
-
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
 
-
| Contact [[BioMicroCenter:People|Manlin]] for training
 
  |-
  |-
  | SYBR Green (KAPA/Roche)
  | SYBR Green (KAPA/Roche)
-
  |align="right"| $34
+
  |align="right"| $35
  |align="right"| NA
  |align="right"| NA
  |align="center"| per ml
  |align="center"| per ml
Line 400: Line 399:
  | Available for MIT only  
  | Available for MIT only  
  |-
  |-
-
  | 96-well RTPCR PLATE
+
  | 96-well PLATES
  |align="right"| $6
  |align="right"| $6
  |align="right"| NA
  |align="right"| NA
Line 408: Line 407:
  |-
  |-
|}
|}
-
prices effective 7.1.2012
+
prices effective 7.1.2013
.
.
<br>
<br>
Line 436: Line 435:
  |align="center"| per session
  |align="center"| per session
  | TBA
  | TBA
-
  | Contact [[BioMicroCenter:People|Shmulik]] for training.
+
  | Contact [[BioMicroCenter:People|Ryan]] for training.
  |-
  |-
| colspan="6" align="center"| '''PLATE READER'''
| colspan="6" align="center"| '''PLATE READER'''
Line 445: Line 444:
  |align="center"| per session
  |align="center"| per session
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
-
  | Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Shmulik]] for training  
+
  | Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Kevin]] for training  
  |-
  |-
  | colspan="6" align="center"| '''ROBOTICS'''
  | colspan="6" align="center"| '''ROBOTICS'''
  |-
  |-
  | Tecan EVO 150
  | Tecan EVO 150
-
  |align="right"| $50
+
  |align="right"| $60
  |align="right"| $150
  |align="right"| $150
  |align="center"| per 2h block - based on scheduled time.
  |align="center"| per 2h block - based on scheduled time.
Line 487: Line 486:
  |-
  |-
|}
|}
-
prices effective 7.1.2012
+
prices effective 7.1.2013
<br><br>
<br><br>
Line 495: Line 494:
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| Other MIT / [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| Other MIT / [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
-
  !width=100| unit
+
  !width=200| unit
  ! Notes
  ! Notes
  |-
  |-
  | Informatics Support
  | Informatics Support
  | align="right"| $70
  | align="right"| $70
-
  | align="right"| $90 / $125
+
  | align="right"| $90 / $135
  | align="right"| per hour
  | align="right"| per hour
  | Limited support for non-MIT users
  | Limited support for non-MIT users
  |-
  |-
-
  | Informatics Share (1 year - 10% FTE)
+
  | Informatics Share
  | align="right"| $960
  | align="right"| $960
-
  | align="right"| NA
+
  | align="right"| N/A
-
  | align="right"| per month
+
  | align="right"| per 0.1FTE
-
  | Contact Stuart Levine or Charlie Whittaker for more information
+
  | MIT only. Priority access to informatics.
  |-
  |-
  | Data storage (1 year)
  | Data storage (1 year)
Line 514: Line 513:
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per TB
  |align="center"| per TB
-
  | (Limited space available)
+
  |  
  |-
  |-
 +
| ROUS node (1 year)
 +
|align="right"| $400
 +
|align="right"| N/A
 +
|align="center"| 8 CPU
 +
| Provides a priority queue for the lab on ROUS.
 +
|-
  | Spotfire License (1 year)
  | Spotfire License (1 year)
  |align="right"| ''Contact Stephen Goldman''
  |align="right"| ''Contact Stephen Goldman''
Line 524: Line 529:
|}
|}
 +
 +
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]]<BR>
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]<BR>
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]<BR>
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]<BR>
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]<BR>
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

Revision as of 11:17, 19 August 2013

Image:BioMicroCenter-header6.jpg

Contents

ILLUMINA SEQUENCING

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer.

ILLUMINA SEQUENCING:HiSeq CORE LAB/MIT Non-MIT unit Notes

HiSEQ 2000 sequencing (minimum 100m reads/lane. Average ~160-240m reads/lane)
Single End (40nt) $1,060 $1,590 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Paired End (40+40nt) $1,575 $2,363 per lane
Add'l 40nt $300 $450 per lane

MiSeq sequencing (minimum 8m reads/lane. Average ~15m reads/lane)
70nt Run $1,100 $1,650 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
300nt Run (PE or SE) $1,300 $1,950 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
500nt Run (PE or SE) $1,500 $2,250 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

GAII / GAIIx sequencing (Average ~25-40m reads/lane)
GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.
8 Lanes (SE/PE) $5,200 / $7,550 $7,800 / $11,325 1 day 24nt per day. 45nt per seq kit.
4 Lanes (SE/PE) $3,800 / $5,075 $5,700 / $7,613 1 day 36nt per day. 66nt per seq kit.
2 Lanes (SE/PE) $3,100 / $4,375 $4,650 / $6,563 1 day 48nt per day. 102nt per seq kit.
1 Lane (SE/PE) $2,750 / NA $4,125 / NA 1 day 72nt per day. 140nt per seq kit.
Add'l Day $600 $900 1 day covers service contract and data storage requirements.
Add'l Sequencing Kit $1,450 $2,175 1 Kit

Additional Services
These services apply to samples submitted for all platforms.
Sample Multiplexing $50 $75 per 10 samples Combined over the whole order. Only applies to samples combined by BMC technicians.
Add'l Repriming $200 $300 per flow cell Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Quality Control Only $25 $38 per sample Includes RT-PCR and BioAnalyzer



Prices effective 7.1.2013

Please note that samples submitted from CORE LABs will be given priority on the Hi-Seq. The BioMicro Center reserves the right to reject samples for sample preparations.

SAMPLE PREPARATION

ILLUMINA Sample Preparation CORE LAB/MIT Non-MIT unit Notes
STARTING WITH DNA
Full Service Sheared DNA (SPRIWORKS) $150 $225 per sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only $100 N/A per sample User must provide all primers. Samples will be returned for amplification.
Full Service Intact Genomic DNA (NEXTERA) $150 $225 per sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
STARTING WITH RNA
Illumina TruSeq $300 $450 per sample Preformed using TruSeq AND SPRIworks system.
Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+strand specific $30 $45 per sample Requires 1ug of RNA. Uses the dUTP method.
Epicenter Ribozero
no polyA pulldown
$350 $525 per sample Requires 1ug of RNA. Based on Illumina Truseq chemistry
Low Input (Clontech) $400 $600 per sample Can work on single cells
Low Input (NuGen) $430 $645 per sample Can work on degraded RNA.
Tight Size Cut $85 $130 per pool +/- 40bp using PippinPrep
Small RNA $275 $413 per sample Uses NEB small RNA kit
STARTING WITH CHROMATIN Note: User must supply antibodies.
Full Service ChIP-seq $210 $315 per sample Preformed using IP-Star AND SPRIworks system.
Includes ChIP, library contruction and barcoding.
IP-Star Only $300 $450 per batch (1-8 samples) Preformed using IP-Star




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MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS CORE LAB MIT Non-MIT unit Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng) $300 $380 $570 per sample Using NuGEN sample labeling kit.
Eukaryote (<20ng) $400 $500 $750 per sample Using NuGEN sample labeling kit.
Prokaryote $365 $455 $683 per sample Using Ambion sample labeling kit.
AGILENT PROCESSING
Expression - ONE COLOR - Eukaryote $200 $250 $375 per array
Expression - TWO COLOR - Eukaryote $290 $340 $510 per array Includes both Cy5 and Cy3
miRNA - ONE COLOR $260 $320 $480 per array
Prokaryote - ONE COLOR $200 $260 $390 per array
Scanning Only $50 $50 N/A per slide Price per glass slide.

prices effective 7.1.2013

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices
  • available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER CORE LAB / MIT Non-MIT unit Notes
RNA / DNA on Advanced Analytical $8 $12 per sample
AA - Preloaded plate $30 per row (12) 2ul per sample in each well using plates from BMC
All DNA & RNA on BIOANALYZER $15 $25 per sample
High Sensitivity protein $40 $60 per sample detection of proteins at < 30ng/ul.

prices effective 7.1.2012
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REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
FLUIDIGM
Fluidigm BioMark $150 $225 per use CALENDER Contact Shmulik
48.48 Dynamic Array Chip $250 N/A per cassette Cassettes are available in the BMC.
96.96 Dynamic Array Chip $700 N/A per cassette Cassettes are available in the BMC.
WAFERGEN SMART CHIP $150 N/A per chip Chips available from BMC Contact Michael
WAFERGEN TRAINING $100 N/A per chip Mandatory Contact Michael
Roche LightCycler 480 $10 NA per plate CALENDER *available for CORE only Contact Michael for training
SYBR Green (KAPA/Roche) $35 NA per ml Available for MIT only
96-well PLATES $6 NA per plate Available for MIT only

prices effective 7.1.2013 .

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
PIPPIN PREP
Gel Isolation $50 $75 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Ryan for training.
PLATE READER
Varioskan $10 $15 per session CALENDER Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Kevin for training
ROBOTICS
Tecan EVO 150 $60 $150 per 2h block - based on scheduled time. CALENDER
non-filter tips $6.00 $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $100 $150 per session Contact Stuart
NANODROP
Nanodrop -- -- Walk up service * no charge

prices effective 7.1.2013

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES CORE LAB Other MIT / Non-MIT unit Notes
Informatics Support $70 $90 / $135 per hour Limited support for non-MIT users
Informatics Share $960 N/A per 0.1FTE MIT only. Priority access to informatics.
Data storage (1 year) $400 N/A per TB
ROUS node (1 year) $400 N/A 8 CPU Provides a priority queue for the lab on ROUS.
Spotfire License (1 year) Contact Stephen Goldman N/A per user (Limited seats available)


FY2014 Pricing
FY2013 Pricing
FY2012 Pricing
FY2011 Pricing

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