BioMicroCenter:Pricing

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(ILLUMINA SEQUENCING)
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{{BioMicroCenter}}
{{BioMicroCenter}}
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Services provided by the BioMicro Center are billed monthly to MIT account numbers (7-digit numbers). If you do not have an MIT account number, you will need to provide a PO number for our services. Cost estimates are available by request.
 
-
 
== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==
== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==
 +
=== SEQUENCING ===
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer.
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer.
-
{| border=1 align="right"
+
{| border=1 align="left"
-
  ! ILLUMINA SEQUENCING
+
  ! ILLUMINA SEQUENCING:HiSeq
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
-
  !width=100| Non-MIT
+
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| unit
  !width=100| unit
  ! Notes
  ! Notes
  |-
  |-
-
  | Single End (36nt)
+
| colspan="5" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 100m reads/lane. Average ~160-240m reads/lane)
-
  |align="right"| $860
+
|-
-
|align="right"| $1,800
+
  | Single End (40nt)
 +
  |align="right"| $1,060
 +
|align="right"| $1,590
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
+
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
  |-
  |-
-
  | Paired End (36+36nt)
+
  | Paired End (40+40nt)
-
  |align="right"| $1,450
+
  |align="right"| $1,575
-
|align="right"| $3,000
+
|align="right"| $2,363
  |align="center"| per lane
  |align="center"| per lane
  |  
  |  
  |-
  |-
-
  | Add'l 36nt
+
  | Add'l 40nt
  |align="right"| $300
  |align="right"| $300
-
  |align="right"| $555
+
  |align="right"| $450
  |align="center"| per lane
  |align="center"| per lane
  |   
  |   
  |-
  |-
 +
| colspan="5" align=center |<BR>'''MiSeq''' sequencing (minimum 8m reads/lane. Average ~15m reads/lane)
 +
|-
 +
| 70nt Run
 +
|align="right"| $1,100
 +
|align="right"| $1,650
 +
|align="center"| per lane
 +
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 +
|-
 +
| 300nt Run (PE or SE)
 +
|align="right"| $1,300
 +
|align="right"| $1,950
 +
|align="center"| per lane
 +
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 +
|-
 +
| 500nt Run (PE or SE)
 +
|align="right"| $1,500
 +
|align="right"| $2,250
 +
|align="center"| per lane
 +
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 +
|-
 +
| colspan="5" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
 +
|-
 +
| colspan="5" align=center |''GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.''
 +
|-
 +
| 8 Lanes (SE/PE)
 +
|align="right"| $5,200 / $7,550
 +
|align="right"| $7,800 / $11,325
 +
|align="center"| 1 day
 +
| 24nt per day. 45nt per seq kit.
 +
|-
 +
| 4 Lanes (SE/PE)
 +
|align="right"| $3,800 / $5,075
 +
|align="right"| $5,700 / $7,613
 +
|align="center"| 1 day
 +
| 36nt per day. 66nt per seq kit.
 +
|-
 +
| 2 Lanes (SE/PE)
 +
|align="right"| $3,100 / $4,375
 +
|align="right"| $4,650 / $6,563
 +
|align="center"| 1 day
 +
| 48nt per day. 102nt per seq kit.
 +
|-
 +
| 1 Lane (SE/PE)
 +
|align="right"| $2,750 / NA
 +
|align="right"| $4,125 / NA
 +
|align="center"| 1 day
 +
| 72nt per day. 140nt per seq kit.
 +
|-
 +
| Add'l Day
 +
|align="right"| $600
 +
|align="right"| $900
 +
|align="center"| 1 day
 +
| covers service contract and data storage requirements.
 +
|-
 +
| Add'l Sequencing Kit
 +
|align="right"| $1,450
 +
|align="right"| $2,175
 +
|align="center"| 1 Kit
 +
|
 +
|-
 +
| colspan="5" align=center |<BR>'''Additional Services'''
 +
|-
 +
| colspan="5" align=center |''These services apply to samples submitted for all platforms.''
 +
|-
  | Sample Multiplexing
  | Sample Multiplexing
  |align="right"| $50
  |align="right"| $50
-
  |align="right"| $100
+
  |align="right"| $75
  |align="center"| per 10 samples
  |align="center"| per 10 samples
-
  | Combined over the whole order. Only applies to samples combined by BCM technicians.
+
  | Combined over the whole order. Only applies to samples combined by BMC technicians.
  |-
  |-
  | Add'l Repriming
  | Add'l Repriming
 +
|align="right"| $200
  |align="right"| $300
  |align="right"| $300
-
|align="right"| $600
 
  |align="center"| per flow cell
  |align="center"| per flow cell
-
  | Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
+
  | Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
 +
|-
 +
| Quality Control Only
 +
|align="right"| $25
 +
|align="right"| $38
 +
|align="center"| per sample
 +
| Includes RT-PCR and BioAnalyzer
  |}
  |}
<BR>
<BR>
<BR>
<BR>
-
Prices effective 4/1/10 <BR>
+
Prices effective 7.1.2013 <BR><BR>
 +
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.
 +
 
 +
=== SAMPLE PREPARATION ===
 +
 
 +
{| border=1 align="left"
 +
! ILLUMINA Sample Preparation
 +
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
 +
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
!width=100| unit
 +
! Notes
 +
|-
 +
| colspan="5" | '''STARTING WITH DNA'''
 +
|-
 +
| Full Service Sheared DNA (SPRIWORKS)
 +
|align="right"| $150
 +
|align="right"| $225
 +
|align="center"| per sample
 +
| Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
| ''SPRI-TE use only''
 +
|align="right"| $100
 +
|align="right"| N/A
 +
|align="center"| per sample
 +
| User must provide all primers. Samples will be returned for amplification.
 +
|-
 +
| Full Service Intact Genomic DNA (NEXTERA)
 +
|align="right"| $150
 +
|align="right"| $225
 +
|align="center"| per sample
 +
| Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
| colspan="5" | '''STARTING WITH RNA'''
 +
|-
 +
| Illumina TruSeq
 +
|align="right"| $300
 +
|align="right"| $450
 +
|align="center"| per sample
 +
| Preformed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
|''+strand specific''
 +
|align="right"| $30
 +
|align="right"| $45
 +
|align="center"| per sample
 +
| Requires 1ug of RNA. Uses the dUTP method.
 +
|-
 +
| Epicenter Ribozero<br>''no polyA pulldown''
 +
|align="right"| $350
 +
|align="right"| $525
 +
|align="center"| per sample
 +
| Requires 1ug of RNA. Based on Illumina Truseq chemistry
 +
|-
 +
| Low Input (Clontech)
 +
|align="right"| $400
 +
|align="right"| $600
 +
|align="center"| per sample
 +
| Can work on single cells
 +
|-
 +
| Low Input (NuGen)
 +
|align="right"| $430
 +
|align="right"| $645
 +
|align="center"| per sample
 +
| Can work on degraded RNA.
 +
|-
 +
| Tight Size Cut
 +
|align="right"| $85
 +
|align="right"| $130
 +
|align="center"| per pool
 +
| +/- 40bp using PippinPrep
 +
|-
 +
| Small RNA
 +
| align="right"|$275
 +
| align="right"|$413
 +
| align="center"|per sample
 +
| Uses NEB small RNA kit
 +
|-
 +
| colspan="5" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
 +
|-
 +
| Full Service ChIP-seq
 +
|align="right"| $210
 +
|align="right"| $315
 +
|align="center"| per sample
 +
| Preformed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
 +
|-
 +
| ''IP-Star Only''
 +
|align="right"| $300
 +
|align="right"| $450
 +
|align="center"| per batch (1-8 samples)
 +
| Preformed using IP-Star
 +
|-
 +
|}
 +
<BR>
 +
<BR>
 +
<BR>
.
.
 +
<BR>
 +
<BR>
 +
<BR>
 +
<BR>
 +
<BR>
== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ==
== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ==
Line 57: Line 225:
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| MIT
  !width=100| MIT
-
  !width=100| Non-MIT
+
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| unit
  !width=100| unit
  ! Notes
  ! Notes
Line 63: Line 231:
  | colspan="6" | '''AFFYMETRIX PROCESSING'''
  | colspan="6" | '''AFFYMETRIX PROCESSING'''
  |-
  |-
-
  | Eukaryote 3' (std)
+
  | Eukaryote (3' or Exon: >20ng)
-
  |align="right"| $260
+
  |align="right"| $300
-
  |align="right"| $430
+
  |align="right"| $380
-
  |align="right"| $645
+
  |align="right"| $570
  |align="center"| per sample
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
  | Using NuGEN sample labeling kit.
  |-
  |-
-
  | Eukaryote Exon
+
  | Eukaryote (<20ng)
-
  |align="right"| $320
+
  |align="right"| $400
  |align="right"| $500
  |align="right"| $500
  |align="right"| $750
  |align="right"| $750
Line 78: Line 246:
  |-
  |-
  | Prokaryote
  | Prokaryote
-
  |align="right"| $250
+
  |align="right"| $365
-
  |align="right"| $410
+
  |align="right"| $455
-
  |align="right"| $615
+
  |align="right"| $683
  |align="center"| per sample
  |align="center"| per sample
-
  | Using Ambion sample labeling kit
+
  | Using Ambion sample labeling kit.   
-
|-
+
-
| Fluidics Station and Scan
+
-
|align="right"| $130
+
-
|align="right"| $130
+
-
|align="right"| N/A
+
-
|align="center"| per array
+
-
| Users must be trained and coordinate with Manlin.   
+
  |-
  |-
  | colspan="6" | '''AGILENT PROCESSING'''
  | colspan="6" | '''AGILENT PROCESSING'''
  |-
  |-
-
  | Eukaryote Expression
+
  | Expression - ONE COLOR - Eukaryote
-
  |align="right"| $270
+
  |align="right"| $200
-
  |align="right"| $430
+
  |align="right"| $250
-
  |align="right"| $645
+
  |align="right"| $375
  |align="center"| per array
  |align="center"| per array
-
  | Includes both Cy5 and Cy3
+
  |  
  |-
  |-
-
  | aCGH
+
  | Expression - TWO COLOR - Eukaryote
-
  |align="right"| $250
+
  |align="right"| $290
-
  |align="right"| $430
+
  |align="right"| $340
-
  |align="right"| $645
+
  |align="right"| $510
  |align="center"| per array
  |align="center"| per array
  | Includes both Cy5 and Cy3
  | Includes both Cy5 and Cy3
  |-
  |-
 +
| miRNA - ONE COLOR
 +
|align="right"| $260
 +
|align="right"| $320
 +
|align="right"| $480
 +
|align="center"| per array
 +
|
 +
|-
 +
| Prokaryote - ONE COLOR
 +
|align="right"| $200
 +
|align="right"| $260
 +
|align="right"| $390
 +
|align="center"| per array
 +
|
 +
|-
  | Scanning Only
  | Scanning Only
  |align="right"| $50
  |align="right"| $50
Line 115: Line 290:
  |-  
  |-  
|}
|}
 +
prices effective 7.1.2013
<br>
<br>
Line 123: Line 299:
  $400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
  $400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
  Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
  Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
-
 
-
'''Common Agilent Expression Arrays*^:'''
 
-
 
-
$807.20  - 8x15k designs (cost per tile, must use all 8 at once)      E.coli / S.cerevisiae / Custom
 
-
$170    - 4x44k designs (cost per tile, must use all 4 at once)      Human / Mouse / C.elegans / Custom
 
-
 
-
 
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.  
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.  
-
 
 
-
^ Discount pricing available through the BioMicro Center.
 
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
Line 138: Line 305:
  ! AGILENT BIOANALYZER
  ! AGILENT BIOANALYZER
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
-
  !width=100| Non-MIT
+
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| unit
  !width=100| unit
  ! Notes
  ! Notes
  |-
  |-
-
  | DNA / RNA / PROTEIN
+
  | RNA / DNA on Advanced Analytical
-
  |align="right"| $10
+
  |align="right"| $8
-
  |align="right"| $15
+
  |align="right"| $12
  |align="center"| per sample
  |align="center"| per sample
  |  
  |  
  |-
  |-
-
  | small RNA / High Sens. DNA
+
  | AA - Preloaded plate
 +
|align="right"| $30
 +
|align="right"| $45
 +
|align="center"| per row (12)
 +
| 2ul per sample in each well using plates from BMC
 +
|-
 +
| All DNA & RNA on BIOANALYZER
  |align="right"| $15
  |align="right"| $15
  |align="right"| $25
  |align="right"| $25
Line 155: Line 328:
  |-
  |-
  | High Sensitivity protein
  | High Sensitivity protein
-
  |align="right"| $25
+
  |align="right"| $40
-
  |align="right"| $35
+
  |align="right"| $60
  |align="center"| per sample
  |align="center"| per sample
  | detection of proteins at < 30ng/ul.   
  | detection of proteins at < 30ng/ul.   
-
|-
 
-
| Flow Cytometry
 
-
|align="right"| $20
 
-
|align="right"| $30
 
-
|align="center"| per sample
 
-
 
  |-
  |-
|}
|}
 +
prices effective 7.1.2012
<br>.
<br>.
-
== OTHER LAB SERVICES ==
+
== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==
{| border=1
{| border=1
  ! OTHER LAB SERVICES
  ! OTHER LAB SERVICES
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
-
  !width=100| Non-MIT
+
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| unit
  !width=100| unit
  ! SIGN UP
  ! SIGN UP
  ! Notes
  ! Notes
  |-
  |-
-
  | colspan="6" align="center"| '''RT-PCR'''
+
  | colspan="6" align="center"| '''FLUIDIGM'''
 +
|-
 +
| Fluidigm BioMark
 +
|align="right"| $150
 +
|align="right"| $225
 +
|align="center"| per use
 +
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
 +
| Contact [[BioMicroCenter:People|Shmulik]]
 +
|-
 +
| 48.48 Dynamic Array Chip
 +
|align="right"| $250
 +
|align="right"| N/A
 +
|align="center"| per cassette
 +
| Cassettes are available in the BMC.
 +
|
 +
|-
 +
| 96.96 Dynamic Array Chip
 +
|align="right"| $700
 +
|align="right"| N/A
 +
|align="center"| per cassette
 +
| Cassettes are available in the BMC.
 +
|
 +
|-
 +
| WAFERGEN SMART CHIP
 +
|align="right"| $150
 +
|align="right"| N/A
 +
|align="center"| per chip
 +
| Chips available from BMC
 +
| Contact [[BioMicroCenter:People|Michael]]
 +
|-
 +
| WAFERGEN TRAINING
 +
|align="right"| $100
 +
|align="right"| N/A
 +
|align="center"| per chip
 +
| Mandatory
 +
| Contact [[BioMicroCenter:People|Michael]]
 +
|- | colspan="6" align="center"| '''RT-PCR'''
  |-
  |-
  | Roche LightCycler 480
  | Roche LightCycler 480
  |align="right"| $10
  |align="right"| $10
-
  |align="right"| $15
+
  |align="right"| NA
  |align="center"| per plate
  |align="center"| per plate
-
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
+
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
-
  | (cost effective July 1, 2009)
+
  | Contact [[BioMicroCenter:People|Michael]] for training
  |-
  |-
-
  | MJ Opticon II
+
  | SYBR Green (KAPA/Roche)
-
  |align="right"| $10
+
  |align="right"| $35
-
  |align="right"| $15
+
|align="right"| NA
 +
|align="center"| per ml
 +
|
 +
| Available for MIT only
 +
|-
 +
| 96-well PLATES
 +
  |align="right"| $6
 +
|align="right"| NA
  |align="center"| per plate
  |align="center"| per plate
-
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
+
  |
 +
| Available for MIT only
 +
|-
 +
|}
 +
prices effective 7.1.2013
 +
.
 +
<br>
 +
 
 +
== OTHER LAB SERVICES ==
 +
 
 +
{| border=1
 +
! OTHER LAB SERVICES
 +
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
 +
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
!width=100| unit
 +
! SIGN UP
 +
! Notes
 +
|-
 +
| colspan="6" align="center"| '''PIPPIN PREP'''
 +
|-
 +
|Gel Isolation
 +
|align="right"| $50
 +
|align="right"| $75
 +
|align="center"| per gel (5 lanes)
 +
| TBA
  |  
  |  
  |-
  |-
-
  | colspan="6" align="center"| '''PLATE READER'''
+
  |Training
 +
|align="right"| $25
 +
|align="right"| $50
 +
|align="center"| per session
 +
| TBA
 +
| Contact [[BioMicroCenter:People|Ryan]] for training.
 +
|-
 +
| colspan="6" align="center"| '''PLATE READER'''
  |-
  |-
  | Varioskan
  | Varioskan
  |align="right"| $10
  |align="right"| $10
  |align="right"| $15
  |align="right"| $15
-
  |align="center"| per plate
+
  |align="center"| per session
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
-
  | Quick scans (those requiring less then 10 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority.
+
  | Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Kevin]] for training
  |-
  |-
  | colspan="6" align="center"| '''ROBOTICS'''
  | colspan="6" align="center"| '''ROBOTICS'''
  |-
  |-
  | Tecan EVO 150
  | Tecan EVO 150
-
  |align="right"| $75
+
  |align="right"| $60
-
  |align="right"| $125
+
  |align="right"| $150
-
  |align="center"| per session
+
  |align="center"| per 2h block - based on scheduled time.
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
-
  | (cost may decrease depending on usage levels)
+
  |  
  |-
  |-
-
  |50ul non-sterile tips
+
  |non-filter tips
-
  |align="right"| $6.50
+
  |align="right"| $6.00
-
  |align="right"| $6.50
+
  |align="right"| $6.00
  |align="center"| per box
  |align="center"| per box
  |
  |
  |** Please let us know if there is additional plasticware you would like us to stock.
  |** Please let us know if there is additional plasticware you would like us to stock.
  |-
  |-
-
  |200ul non-sterile tips
+
  |Filter tips
-
  |align="right"| $5.00
+
  |align="right"| $8.00
-
  |align="right"| $5.00
+
  |align="right"| $8.00
-
|align="center"| per box
+
-
|
+
-
|
+
-
|-
+
-
|200ul sterile tips
+
-
|align="right"| $5.50
+
-
|align="right"| $5.50
+
  |align="center"| per box
  |align="center"| per box
  |
  |
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  |align="right"| $150
  |align="right"| $150
  |align="center"| per session
  |align="center"| per session
-
  | Contact [[BioMicroCenter:People|Manlin]
+
  | Contact [[BioMicroCenter:People|Stuart]]
  |  
  |  
  |-
  |-
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  |-
  |-
|}
|}
 +
prices effective 7.1.2013
<br><br>
<br><br>
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  ! COMPUTATIONAL SERVICES
  ! COMPUTATIONAL SERVICES
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
-
  !width=100| Other MIT / Non-MIT
+
  !width=100| Other MIT / [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
-
  !width=100| unit
+
  !width=200| unit
  ! Notes
  ! Notes
 +
|-
 +
| Informatics Support
 +
| align="right"| $70
 +
| align="right"| $90 / $135
 +
| align="right"| per hour
 +
| Limited support for non-MIT users
 +
|-
 +
| Informatics Share
 +
| align="right"| $960
 +
| align="right"| N/A
 +
| align="right"| per 0.1FTE
 +
| MIT only. Priority access to informatics.
  |-
  |-
  | Data storage (1 year)
  | Data storage (1 year)
-
  |align="right"| $500
+
  |align="right"| $400
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per TB
  |align="center"| per TB
-
  | (Limited space available)
+
|
 +
|-
 +
| ROUS node (1 year)
 +
|align="right"| $400
 +
|align="right"| N/A
 +
|align="center"| 8 CPU
 +
| Provides a priority queue for the lab on ROUS.
 +
|-
 +
| Spotfire License (1 year)
 +
|align="right"| ''Contact Stephen Goldman''
 +
|align="right"| N/A
 +
|align="center"| per user
 +
  | (Limited seats available)
  |-
  |-
|}
|}
 +
 +
 +
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]]<BR>
 +
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]<BR>
 +
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]<BR>
 +
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

Revision as of 21:06, 1 July 2013

Image:BioMicroCenter-header6.jpg

Contents

ILLUMINA SEQUENCING

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer.

ILLUMINA SEQUENCING:HiSeq CORE LAB/MIT Non-MIT unit Notes

HiSEQ 2000 sequencing (minimum 100m reads/lane. Average ~160-240m reads/lane)
Single End (40nt) $1,060 $1,590 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Paired End (40+40nt) $1,575 $2,363 per lane
Add'l 40nt $300 $450 per lane

MiSeq sequencing (minimum 8m reads/lane. Average ~15m reads/lane)
70nt Run $1,100 $1,650 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
300nt Run (PE or SE) $1,300 $1,950 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
500nt Run (PE or SE) $1,500 $2,250 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

GAII / GAIIx sequencing (Average ~25-40m reads/lane)
GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.
8 Lanes (SE/PE) $5,200 / $7,550 $7,800 / $11,325 1 day 24nt per day. 45nt per seq kit.
4 Lanes (SE/PE) $3,800 / $5,075 $5,700 / $7,613 1 day 36nt per day. 66nt per seq kit.
2 Lanes (SE/PE) $3,100 / $4,375 $4,650 / $6,563 1 day 48nt per day. 102nt per seq kit.
1 Lane (SE/PE) $2,750 / NA $4,125 / NA 1 day 72nt per day. 140nt per seq kit.
Add'l Day $600 $900 1 day covers service contract and data storage requirements.
Add'l Sequencing Kit $1,450 $2,175 1 Kit

Additional Services
These services apply to samples submitted for all platforms.
Sample Multiplexing $50 $75 per 10 samples Combined over the whole order. Only applies to samples combined by BMC technicians.
Add'l Repriming $200 $300 per flow cell Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Quality Control Only $25 $38 per sample Includes RT-PCR and BioAnalyzer



Prices effective 7.1.2013

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.

SAMPLE PREPARATION

ILLUMINA Sample Preparation CORE LAB/MIT Non-MIT unit Notes
STARTING WITH DNA
Full Service Sheared DNA (SPRIWORKS) $150 $225 per sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only $100 N/A per sample User must provide all primers. Samples will be returned for amplification.
Full Service Intact Genomic DNA (NEXTERA) $150 $225 per sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
STARTING WITH RNA
Illumina TruSeq $300 $450 per sample Preformed using TruSeq AND SPRIworks system.
Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+strand specific $30 $45 per sample Requires 1ug of RNA. Uses the dUTP method.
Epicenter Ribozero
no polyA pulldown
$350 $525 per sample Requires 1ug of RNA. Based on Illumina Truseq chemistry
Low Input (Clontech) $400 $600 per sample Can work on single cells
Low Input (NuGen) $430 $645 per sample Can work on degraded RNA.
Tight Size Cut $85 $130 per pool +/- 40bp using PippinPrep
Small RNA $275 $413 per sample Uses NEB small RNA kit
STARTING WITH CHROMATIN Note: User must supply antibodies.
Full Service ChIP-seq $210 $315 per sample Preformed using IP-Star AND SPRIworks system.
Includes ChIP, library contruction and barcoding.
IP-Star Only $300 $450 per batch (1-8 samples) Preformed using IP-Star




.




MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS CORE LAB MIT Non-MIT unit Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng) $300 $380 $570 per sample Using NuGEN sample labeling kit.
Eukaryote (<20ng) $400 $500 $750 per sample Using NuGEN sample labeling kit.
Prokaryote $365 $455 $683 per sample Using Ambion sample labeling kit.
AGILENT PROCESSING
Expression - ONE COLOR - Eukaryote $200 $250 $375 per array
Expression - TWO COLOR - Eukaryote $290 $340 $510 per array Includes both Cy5 and Cy3
miRNA - ONE COLOR $260 $320 $480 per array
Prokaryote - ONE COLOR $200 $260 $390 per array
Scanning Only $50 $50 N/A per slide Price per glass slide.

prices effective 7.1.2013

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices
  • available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER CORE LAB / MIT Non-MIT unit Notes
RNA / DNA on Advanced Analytical $8 $12 per sample
AA - Preloaded plate $30 $45 per row (12) 2ul per sample in each well using plates from BMC
All DNA & RNA on BIOANALYZER $15 $25 per sample
High Sensitivity protein $40 $60 per sample detection of proteins at < 30ng/ul.

prices effective 7.1.2012
.

REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
FLUIDIGM
Fluidigm BioMark $150 $225 per use CALENDER Contact Shmulik
48.48 Dynamic Array Chip $250 N/A per cassette Cassettes are available in the BMC.
96.96 Dynamic Array Chip $700 N/A per cassette Cassettes are available in the BMC.
WAFERGEN SMART CHIP $150 N/A per chip Chips available from BMC Contact Michael
WAFERGEN TRAINING $100 N/A per chip Mandatory Contact Michael
Roche LightCycler 480 $10 NA per plate CALENDER *available for CORE only Contact Michael for training
SYBR Green (KAPA/Roche) $35 NA per ml Available for MIT only
96-well PLATES $6 NA per plate Available for MIT only

prices effective 7.1.2013 .

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
PIPPIN PREP
Gel Isolation $50 $75 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Ryan for training.
PLATE READER
Varioskan $10 $15 per session CALENDER Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Kevin for training
ROBOTICS
Tecan EVO 150 $60 $150 per 2h block - based on scheduled time. CALENDER
non-filter tips $6.00 $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $100 $150 per session Contact Stuart
NANODROP
Nanodrop -- -- Walk up service * no charge

prices effective 7.1.2013

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES CORE LAB Other MIT / Non-MIT unit Notes
Informatics Support $70 $90 / $135 per hour Limited support for non-MIT users
Informatics Share $960 N/A per 0.1FTE MIT only. Priority access to informatics.
Data storage (1 year) $400 N/A per TB
ROUS node (1 year) $400 N/A 8 CPU Provides a priority queue for the lab on ROUS.
Spotfire License (1 year) Contact Stephen Goldman N/A per user (Limited seats available)


FY2014 Pricing
FY2013 Pricing
FY2012 Pricing
FY2011 Pricing

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