BioMicroCenter:Pricing

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ILLUMINA SEQUENCING

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer.

ILLUMINA SEQUENCING:HiSeq	CORE LAB/MIT	Non-MIT	unit	Notes
HiSEQ 2000 sequencing (minimum 100m reads/lane. Average ~160-240m reads/lane)
Single End (40nt)	$1,170	$1,755	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 yrs.
Paired End (40+40nt)	$1,850	$2,775	per lane
Add'l 40nt	$370	$555	per lane
MiSeq sequencing (minimum 2m reads/lane. Average ~5m reads/lane)
50nt Run	$1,000	$1,500	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
300nt Run (PE or SE)	$1,300	$1,950	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
500nt Run (PE or SE)	$1,450	$2,175	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
GAII / GAIIx sequencing (Average ~25-40m reads/lane)
GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.
8 Lanes (SE/PE)	$4,835 / $7,135	$7,253 / $10,702	1 day	24nt per day. 42nt per seq kit.
4 Lanes (SE/PE)	$3,465 / $4,700	$5,198 / $7,050	1 day	36nt per day. 72nt per seq kit.
2 Lanes (SE/PE)	$2,780 / $4,015	$4,170 / $6,023	1 day	48nt per day. 120nt per seq kit.
1 Lane (SE/PE)	$2,440 / $3,675	$3,660 / $5,513	1 day	72nt per day. 180nt per seq kit.
Add'l Day	$325	$488	1 day	covers service contract and data storage requirements.
Add'l Sequencing Kit	$1,400	$2,100	1 Kit
Additional Services
These services apply to samples submitted for all platforms.
Sample Multiplexing	$50	$75	per 10 samples	Combined over the whole order. Only applies to samples combined by BMC technicians.
Add'l Repriming	$200	$300	per flow cell	Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Quality Control Only	$30	$45	per sample	Includes RT-PCR and BioAnalyzer

Prices effective 7.1.2012

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.

SAMPLE PREPARATION

ILLUMINA Sample Preparation	CORE LAB/MIT	Non-MIT	unit	Notes
STARTING WITH DNA
Full Service Sheared DNA (SPRIWORKS)	$150	$225	per sample	Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only	$100	N/A	per sample	User must provide all primers. We do not QC the samples. Samples will be returned for amplification.
Full Service Intact Genomic DNA (NEXTERA)	$150	$225	per sample	Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
STARTING WITH RNA
Illumina TruSeq	$300	$450	per sample	Preformed using TruSeq AND SPRIworks system. Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+strand specific	$30	$45	per sample	Requires 1ug of RNA. Uses the dUTP method.
Epicenter Ribozero no polyA pulldown	$350	$525	per sample	Requires 1ug of RNA. Based on Illumina Truseq chemistry
Low Input (Clontech)	$375	$575	per sample	Can work on single cells
Low Input (NuGen)	$400	$600	per sample	Can work on degraded RNA.
Tight Size Cut	$75	$125	per pool	+/- 40bp using PippinPrep
STARTING WITH CHROMATIN Note: User must supply antibodies.
Full Service ChIP-seq	$210	$315	per sample	Preformed using IP-Star AND SPRIworks system. Includes ChIP, library contruction and barcoding.
IP-Star Only	$70	$105	per sample	Preformed using IP-Star
qPCR of ChIP in development	TBA	TBA	per sample	Tests 8 IPs against 5 genomic loci in duplicate. User provides oligos.

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MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS	CORE LAB	MIT	Non-MIT	unit	Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng)	$270	$360	$540	per sample	Using NuGEN sample labeling kit.
Eukaryote (<20ng)	$370	$460	$690	per sample	Using NuGEN sample labeling kit.
Prokaryote	$365	$455	$680	per sample	Using Ambion sample labeling kit.
Fluidics Station and Scan	$140	$165	N/A	per array	Users must be trained and coordinate with Manlin.
AGILENT PROCESSING
Expression - ONE COLOR - Eukaryote	$180	$240	$360	per array
Expression - TWO COLOR - Eukaryote	$250	$300	$450	per array	Includes both Cy5 and Cy3
miRNA - ONE COLOR	$230	$300	$450	per array
Prokaryote - ONE COLOR	$230	$300	$450	per array
Scanning Only	$50	$50	N/A	per slide	Price per glass slide.

prices effective 7.1.2012

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices

• available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER	CORE LAB / MIT	Non-MIT	unit	Notes
High Sensitivity DNA or Nano RNA	$10	$25	per sample
All other DNA and RNA samples	$15	$25	per sample
High Sensitivity protein	$40	$60	per sample	detection of proteins at < 30ng/ul.
Flow Cytometry	by request	NA	per sample

prices effective 7.1.2012
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REAL TIME PCR

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
FLUIDIGM
Fluidigm BioMark	$150	$225	per use	CALENDER	Contact Shmulik
48.48 Dynamic Array Chip	$260	N/A	per cassette	Cassettes are available in the BMC.	Contact Shmulik
96.96 Dynamic Array Chip	$760	N/A	per cassette	Cassettes are available in the BMC.	Contact Shmulik
RT-PCR
Roche LightCycler 480	$10	NA	per plate	CALENDER *available for CORE only	Contact Michael for training
MJ Opticon II	$10	$15	per plate	CALENDER	Contact Manlin for training
SYBR Green (KAPA/Roche)	$34	NA	per ml		Available for MIT only
96-well RTPCR PLATE	$6	NA	per plate		Available for MIT only

prices effective 7.1.2012 .

OTHER LAB SERVICES

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
PIPPIN PREP
Gel Isolation	$50	$75	per gel (5 lanes)	TBA
Training	$25	$50	per session	TBA	Contact Shmulik for training.
PLATE READER
Varioskan	$10	$15	per session	CALENDER	Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Shmulik for training
ROBOTICS
Tecan EVO 150	$50	$150	per 2h block - based on scheduled time.	CALENDER
non-filter tips	$6.00	$6.00	per box		** Please let us know if there is additional plasticware you would like us to stock.
Filter tips	$8.00	$8.00	per box
Training	$100	$150	per session	Contact Stuart
NANODROP
Nanodrop	--	--		Walk up service	* no charge

prices effective 7.1.2012

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES	CORE LAB	Other MIT / Non-MIT	unit	Notes
Informatics Support	$65	$85 / $120	per hour	Limited support for non-MIT users
Data storage (1 year)	$400	N/A	per TB	(Limited space available)
Spotfire License (1 year)	Contact Stephen Goldman	N/A	per user	(Limited seats available)

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