BioMicroCenter:Pricing

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{{BioMicroCenter}}
{{BioMicroCenter}}
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Services provided by the BioMicro Center are billed monthly to MIT account numbers (7-digit numbers). If you do not have an MIT account number, you will need to provide a PO number for our services. Cost estimates are available by request.
 
-
 
== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==
== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==
 +
=== SEQUENCING ===
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer.
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer.
-
{| border=1 align="right"
+
{| border=1 align="left"
-
  ! ILLUMINA SEQUENCING
+
  ! ILLUMINA SEQUENCING:HiSeq
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
-
  !width=100| Non-MIT
+
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| unit
  !width=100| unit
  ! Notes
  ! Notes
  |-
  |-
-
  | Single End (36nt)
+
| colspan="5" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 100m reads/lane. Average ~160-240m reads/lane)
-
  |align="right"| $860
+
|-
-
  |align="right"| $1,290
+
  | Single End (40nt)
 +
  |align="right"| $1,170
 +
  |align="right"| $1,755
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
+
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 yrs.
  |-
  |-
-
  | Paired End (36+36nt)
+
  | Paired End (40+40nt)
-
  |align="right"| $1,500
+
  |align="right"| $1,850
-
|align="right"| $2,250
+
|align="right"| $2,775
  |align="center"| per lane
  |align="center"| per lane
  |  
  |  
  |-
  |-
-
  | Add'l 36nt
+
  | Add'l 40nt
-
  |align="right"| $300
+
  |align="right"| $370
-
  |align="right"| $450
+
  |align="right"| $555
  |align="center"| per lane
  |align="center"| per lane
  |   
  |   
  |-
  |-
 +
| colspan="5" align=center |<BR>'''MiSeq''' sequencing (minimum 2m reads/lane. Average ~5m reads/lane)
 +
|-
 +
| 50nt Run
 +
|align="right"| $1,000
 +
|align="right"| $1,500
 +
|align="center"| per lane
 +
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
 +
|-
 +
| 300nt Run (PE or SE)
 +
|align="right"| $1,300
 +
|align="right"| $1,950
 +
|align="center"| per lane
 +
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
 +
|-
 +
| 500nt Run (PE or SE)
 +
|align="right"| $1,450
 +
|align="right"| $2,175
 +
|align="center"| per lane
 +
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
 +
|-
 +
| colspan="5" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
 +
|-
 +
| colspan="5" align=center |''GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.''
 +
|-
 +
| 8 Lanes (SE/PE)
 +
|align="right"| $4,835 / $7,135
 +
|align="right"| $7,253 / $10,702
 +
|align="center"| 1 day
 +
| 24nt per day. 42nt per seq kit.
 +
|-
 +
| 4 Lanes (SE/PE)
 +
|align="right"| $3,465 / $4,700
 +
|align="right"| $5,198 / $7,050
 +
|align="center"| 1 day
 +
| 36nt per day. 72nt per seq kit.
 +
|-
 +
| 2 Lanes (SE/PE)
 +
|align="right"| $2,780 / $4,015
 +
|align="right"| $4,170 / $6,023
 +
|align="center"| 1 day
 +
| 48nt per day. 120nt per seq kit.
 +
|-
 +
| 1 Lane (SE/PE)
 +
|align="right"| $2,440 / $3,675
 +
|align="right"| $3,660 / $5,513
 +
|align="center"| 1 day
 +
| 72nt per day. 180nt per seq kit.
 +
|-
 +
| Add'l Day
 +
|align="right"| $325
 +
|align="right"| $488
 +
|align="center"| 1 day
 +
| covers service contract and data storage requirements.
 +
|-
 +
| Add'l Sequencing Kit
 +
|align="right"| $1,400
 +
|align="right"| $2,100
 +
|align="center"| 1 Kit
 +
|
 +
|-
 +
| colspan="5" align=center |<BR>'''Additional Services'''
 +
|-
 +
| colspan="5" align=center |''These services apply to samples submitted for all platforms.''
 +
|-
  | Sample Multiplexing
  | Sample Multiplexing
  |align="right"| $50
  |align="right"| $50
Line 39: Line 103:
  |-
  |-
  | Add'l Repriming
  | Add'l Repriming
 +
|align="right"| $200
  |align="right"| $300
  |align="right"| $300
-
|align="right"| $450
 
  |align="center"| per flow cell
  |align="center"| per flow cell
-
  | Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
+
  | Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
  |-
  |-
  | Quality Control Only
  | Quality Control Only
-
  |align="right"| $40
+
  |align="right"| $30
-
  |align="right"| $60
+
  |align="right"| $45
  |align="center"| per sample
  |align="center"| per sample
-
  |  
+
  | Includes RT-PCR and BioAnalyzer
  |}
  |}
<BR>
<BR>
<BR>
<BR>
-
Prices effective 7.1.2010 <BR>
+
Prices effective 7.1.2012 <BR><BR>
 +
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.
 +
 
 +
=== SAMPLE PREPARATION ===
 +
 
 +
{| border=1 align="left"
 +
! ILLUMINA Sample Preparation
 +
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
 +
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
!width=100| unit
 +
! Notes
 +
|-
 +
| colspan="5" | '''STARTING WITH DNA'''
 +
|-
 +
| Full Service Sheared DNA (SPRIWORKS)
 +
|align="right"| $150
 +
|align="right"| $225
 +
|align="center"| per sample
 +
| Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
| ''SPRI-TE use only''
 +
|align="right"| $100
 +
|align="right"| N/A
 +
|align="center"| per sample
 +
| User must provide all primers. Samples will be returned for amplification.
 +
|-
 +
| Full Service Intact Genomic DNA (NEXTERA)
 +
|align="right"| $150
 +
|align="right"| $225
 +
|align="center"| per sample
 +
| Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
| colspan="5" | '''STARTING WITH RNA'''
 +
|-
 +
| Illumina TruSeq
 +
|align="right"| $300
 +
|align="right"| $450
 +
|align="center"| per sample
 +
| Preformed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 +
|-
 +
|''+strand specific''
 +
|align="right"| $30
 +
|align="right"| $45
 +
|align="center"| per sample
 +
| Requires 1ug of RNA. Uses the dUTP method.
 +
|-
 +
| Epicenter Ribozero<br>''no polyA pulldown''
 +
|align="right"| $350
 +
|align="right"| $525
 +
|align="center"| per sample
 +
| Requires 1ug of RNA. Based on Illumina Truseq chemistry
 +
|-
 +
| Low Input (Clontech)
 +
|align="right"| $375
 +
|align="right"| $575
 +
|align="center"| per sample
 +
| Can work on single cells
 +
|-
 +
| Low Input (NuGen)
 +
|align="right"| $400
 +
|align="right"| $600
 +
|align="center"| per sample
 +
| Can work on degraded RNA.
 +
|-
 +
| Tight Size Cut
 +
|align="right"| $75
 +
|align="right"| $125
 +
|align="center"| per pool
 +
| +/- 40bp using PippinPrep
 +
|-
 +
| colspan="5" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
 +
|-
 +
| Full Service ChIP-seq
 +
|align="right"| $210
 +
|align="right"| $315
 +
|align="center"| per sample
 +
| Preformed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
 +
|-
 +
| ''IP-Star Only''
 +
|align="right"| $70
 +
|align="right"| $105
 +
|align="center"| per sample
 +
| Preformed using IP-Star
 +
|-
 +
| ''qPCR of ChIP''<br> ''in development''
 +
|align="right"| TBA
 +
|align="right"| TBA
 +
|align="center"| per sample
 +
|Tests 8 IPs against 5 genomic loci in duplicate. User provides oligos.
 +
|-
 +
|}
 +
<BR>
 +
<BR>
 +
<BR>
.
.
 +
<BR>
 +
<BR>
 +
<BR>
 +
<BR>
 +
<BR>
== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ==
== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ==
Line 63: Line 225:
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| MIT
  !width=100| MIT
-
  !width=100| Non-MIT
+
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| unit
  !width=100| unit
  ! Notes
  ! Notes
Line 69: Line 231:
  | colspan="6" | '''AFFYMETRIX PROCESSING'''
  | colspan="6" | '''AFFYMETRIX PROCESSING'''
  |-
  |-
-
  | Eukaryote 3' (std)
+
  | Eukaryote (3' or Exon: >20ng)
-
  |align="right"| $280
+
  |align="right"| $270
-
  |align="right"| $350
+
  |align="right"| $360
-
  |align="right"| $525
+
  |align="right"| $540
  |align="center"| per sample
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
  | Using NuGEN sample labeling kit.
  |-
  |-
-
  | Eukaryote Exon
+
  | Eukaryote (<20ng)
-
  |align="right"| $320
+
  |align="right"| $370
-
  |align="right"| $420
+
  |align="right"| $460
-
  |align="right"| $630
+
  |align="right"| $690
  |align="center"| per sample
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
  | Using NuGEN sample labeling kit.
  |-
  |-
  | Prokaryote
  | Prokaryote
-
  |align="right"| $260
+
  |align="right"| $365
-
  |align="right"| $360
+
  |align="right"| $455
-
  |align="right"| $540
+
  |align="right"| $680
  |align="center"| per sample
  |align="center"| per sample
  | Using Ambion sample labeling kit.   
  | Using Ambion sample labeling kit.   
  |-
  |-
  | Fluidics Station and Scan
  | Fluidics Station and Scan
-
  |align="right"| $100
+
  |align="right"| $140
-
  |align="right"| $125
+
  |align="right"| $165
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per array
  |align="center"| per array
Line 99: Line 261:
  | colspan="6" | '''AGILENT PROCESSING'''
  | colspan="6" | '''AGILENT PROCESSING'''
  |-
  |-
-
  | Expression - ONE COLOR
+
  | Expression - ONE COLOR - Eukaryote
-
  |align="right"| $230
+
  |align="right"| $180
-
  |align="right"| $315
+
  |align="right"| $240
-
  |align="right"| $480
+
  |align="right"| $360
  |align="center"| per array
  |align="center"| per array
  |  
  |  
  |-
  |-
-
  | Expression and CGH - TWO COLOR
+
  | Expression - TWO COLOR - Eukaryote
-
  |align="right"| $335
+
  |align="right"| $250
-
  |align="right"| $420
+
  |align="right"| $300
-
  |align="right"| $630
+
  |align="right"| $450
  |align="center"| per array
  |align="center"| per array
  | Includes both Cy5 and Cy3
  | Includes both Cy5 and Cy3
  |-
  |-
  | miRNA - ONE COLOR
  | miRNA - ONE COLOR
-
  |align="right"| $250
+
  |align="right"| $230
-
  |align="right"| $295
+
  |align="right"| $300
 +
|align="right"| $450
 +
|align="center"| per array
 +
|
 +
|-
 +
| Prokaryote - ONE COLOR
 +
|align="right"| $230
 +
|align="right"| $300
  |align="right"| $450
  |align="right"| $450
  |align="center"| per array
  |align="center"| per array
Line 128: Line 297:
  |-  
  |-  
|}
|}
-
prices effective 7.1.2010
+
prices effective 7.1.2012
<br>
<br>
Line 137: Line 306:
  $400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
  $400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
  Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
  Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
-
 
+
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.
-
'''Common Agilent Expression Arrays*^:'''
+
-
 
+
-
$807.20  - 8x15k designs (cost per tile, must use all 8 at once)      E.coli / S.cerevisiae / Custom
+
-
$170    - 4x44k designs (cost per tile, must use all 4 at once)      Human / Mouse / C.elegans / Custom
+
-
 
+
-
 
+
-
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.
+
-
 
+
-
^ Discount pricing available through the BioMicro Center.
+
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
Line 152: Line 312:
  ! AGILENT BIOANALYZER
  ! AGILENT BIOANALYZER
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT
-
  !width=100| Non-MIT
+
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| unit
  !width=100| unit
  ! Notes
  ! Notes
  |-
  |-
-
  | All DNA and RNA samples
+
| High Sensitivity DNA or Nano RNA
 +
|align="right"| $10
 +
|align="right"| $25
 +
|align="center"| per sample
 +
|
 +
|-
 +
  | All other DNA and RNA samples
  |align="right"| $15
  |align="right"| $15
  |align="right"| $25
  |align="right"| $25
Line 175: Line 341:
  |-
  |-
|}
|}
-
prices effective 7.1.2010
+
prices effective 7.1.2012
<br>.
<br>.
Line 183: Line 349:
  ! OTHER LAB SERVICES
  ! OTHER LAB SERVICES
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
-
  !width=100| Non-MIT
+
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| unit
  !width=100| unit
  ! SIGN UP
  ! SIGN UP
  ! Notes
  ! Notes
 +
|-
 +
| colspan="6" align="center"| '''FLUIDIGM'''
 +
|-
 +
| Fluidigm BioMark
 +
|align="right"| $150
 +
|align="right"| $225
 +
|align="center"| per use
 +
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
 +
| Contact [[BioMicroCenter:People|Shmulik]]
 +
|-
 +
| 48.48 Dynamic Array Chip
 +
|align="right"| $260
 +
|align="right"| N/A
 +
|align="center"| per cassette
 +
| Cassettes are available in the BMC.
 +
| Contact [[BioMicroCenter:People|Shmulik]]
 +
|-
 +
| 96.96 Dynamic Array Chip
 +
|align="right"| $760
 +
|align="right"| N/A
 +
|align="center"| per cassette
 +
| Cassettes are available in the BMC.
 +
| Contact [[BioMicroCenter:People|Shmulik]]
  |-
  |-
  | colspan="6" align="center"| '''RT-PCR'''
  | colspan="6" align="center"| '''RT-PCR'''
Line 192: Line 381:
  | Roche LightCycler 480
  | Roche LightCycler 480
  |align="right"| $10
  |align="right"| $10
-
  |align="right"| $15
+
  |align="right"| NA
  |align="center"| per plate
  |align="center"| per plate
-
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
+
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
-
  | Contact [[BioMicroCenter:People|Allison]] for training
+
  | Contact [[BioMicroCenter:People|Michael]] for training
  |-
  |-
  | MJ Opticon II
  | MJ Opticon II
Line 204: Line 393:
  | Contact [[BioMicroCenter:People|Manlin]] for training
  | Contact [[BioMicroCenter:People|Manlin]] for training
  |-
  |-
-
  | Fluidigm BioMark
+
  | SYBR Green (KAPA/Roche)
-
  |align="right"| $100
+
  |align="right"| $34
-
  |align="right"| $150
+
  |align="right"| NA
-
  |align="center"| per cassette
+
  |align="center"| per ml
-
  | Cassettes will be available in BMC soon.
+
  |
-
  | Contact [[BioMicroCenter:People|Manlin]]
+
  | Available for MIT only
 +
|-
 +
| 96-well RTPCR PLATE
 +
|align="right"| $6
 +
|align="right"| NA
 +
|align="center"| per plate
 +
|
 +
| Available for MIT only
  |-
  |-
|}
|}
 +
prices effective 7.1.2012
 +
.
 +
<br>
== OTHER LAB SERVICES ==
== OTHER LAB SERVICES ==
Line 218: Line 417:
  ! OTHER LAB SERVICES
  ! OTHER LAB SERVICES
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
-
  !width=100| Non-MIT
+
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| unit
  !width=100| unit
  ! SIGN UP
  ! SIGN UP
  ! Notes
  ! Notes
  |-
  |-
-
  | colspan="6" align="center"| '''PLATE READER'''
+
  | colspan="6" align="center"| '''PIPPIN PREP'''
 +
|-
 +
|Gel Isolation
 +
|align="right"| $50
 +
|align="right"| $75
 +
|align="center"| per gel (5 lanes)
 +
| TBA
 +
|
 +
|-
 +
|Training
 +
|align="right"| $25
 +
|align="right"| $50
 +
|align="center"| per session
 +
| TBA
 +
| Contact [[BioMicroCenter:People|Shmulik]] for training.
 +
|-
 +
| colspan="6" align="center"| '''PLATE READER'''
  |-
  |-
  | Varioskan
  | Varioskan
  |align="right"| $10
  |align="right"| $10
  |align="right"| $15
  |align="right"| $15
-
  |align="center"| per plate
+
  |align="center"| per session
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
-
  | Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Kevin]] for training  
+
  | Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Shmulik]] for training  
  |-
  |-
  | colspan="6" align="center"| '''ROBOTICS'''
  | colspan="6" align="center"| '''ROBOTICS'''
  |-
  |-
  | Tecan EVO 150
  | Tecan EVO 150
-
  |align="right"| $75
+
  |align="right"| $50
-
  |align="right"| $125
+
  |align="right"| $150
-
  |align="center"| per session
+
  |align="center"| per 2h block - based on scheduled time.
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
-
  | (cost may decrease depending on usage levels)
+
  |  
  |-
  |-
  |non-filter tips
  |non-filter tips
-
  |align="right"| $5.00
+
  |align="right"| $6.00
-
  |align="right"| $5.00
+
  |align="right"| $6.00
  |align="center"| per box
  |align="center"| per box
  |
  |
Line 259: Line 474:
  |align="right"| $150
  |align="right"| $150
  |align="center"| per session
  |align="center"| per session
-
  | Contact [[BioMicroCenter:People|Stuart Levine]]
+
  | Contact [[BioMicroCenter:People|Stuart]]
  |  
  |  
-
|-
 
-
| colspan="6" align="center"| '''NANOSTRING'''
 
-
|-
 
-
| Nanostring
 
-
|align="right"| $10.00
 
-
|align="right"| N/A
 
-
|align="center"| per sample
 
-
| Contact [[BioMicroCenter:People|Manlin Luo]]
 
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| ** available on a trial basis
 
  |-
  |-
  | colspan="6" align="center"| '''NANODROP'''
  | colspan="6" align="center"| '''NANODROP'''
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  |-
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prices effective 7.1.2010
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prices effective 7.1.2012
<br><br>
<br><br>
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  ! COMPUTATIONAL SERVICES
  ! COMPUTATIONAL SERVICES
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
-
  !width=100| Other MIT / Non-MIT
+
  !width=100| Other MIT / [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| unit
  !width=100| unit
  ! Notes
  ! Notes
 +
|-
 +
| Informatics Support
 +
| align="right"| $65
 +
| align="right"| $85 / $120
 +
| align="right"| per hour
 +
| Limited support for non-MIT users
  |-
  |-
  | Data storage (1 year)
  | Data storage (1 year)
-
  |align="right"| $500
+
  |align="right"| $400
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per TB
  |align="center"| per TB
  | (Limited space available)
  | (Limited space available)
 +
|-
 +
| Spotfire License (1 year)
 +
|align="right"| ''Contact Stephen Goldman''
 +
|align="right"| N/A
 +
|align="center"| per user
 +
| (Limited seats available)
  |-
  |-
|}
|}
 +
 +
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]<BR>
 +
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]<BR>
 +
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

Revision as of 21:24, 16 November 2012

Image:BioMicroCenter-header6.jpg

Contents

ILLUMINA SEQUENCING

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer.

ILLUMINA SEQUENCING:HiSeq CORE LAB/MIT Non-MIT unit Notes

HiSEQ 2000 sequencing (minimum 100m reads/lane. Average ~160-240m reads/lane)
Single End (40nt) $1,170 $1,755 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 yrs.
Paired End (40+40nt) $1,850 $2,775 per lane
Add'l 40nt $370 $555 per lane

MiSeq sequencing (minimum 2m reads/lane. Average ~5m reads/lane)
50nt Run $1,000 $1,500 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
300nt Run (PE or SE) $1,300 $1,950 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years
500nt Run (PE or SE) $1,450 $2,175 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years

GAII / GAIIx sequencing (Average ~25-40m reads/lane)
GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.
8 Lanes (SE/PE) $4,835 / $7,135 $7,253 / $10,702 1 day 24nt per day. 42nt per seq kit.
4 Lanes (SE/PE) $3,465 / $4,700 $5,198 / $7,050 1 day 36nt per day. 72nt per seq kit.
2 Lanes (SE/PE) $2,780 / $4,015 $4,170 / $6,023 1 day 48nt per day. 120nt per seq kit.
1 Lane (SE/PE) $2,440 / $3,675 $3,660 / $5,513 1 day 72nt per day. 180nt per seq kit.
Add'l Day $325 $488 1 day covers service contract and data storage requirements.
Add'l Sequencing Kit $1,400 $2,100 1 Kit

Additional Services
These services apply to samples submitted for all platforms.
Sample Multiplexing $50 $75 per 10 samples Combined over the whole order. Only applies to samples combined by BMC technicians.
Add'l Repriming $200 $300 per flow cell Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Quality Control Only $30 $45 per sample Includes RT-PCR and BioAnalyzer



Prices effective 7.1.2012

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.

SAMPLE PREPARATION

ILLUMINA Sample Preparation CORE LAB/MIT Non-MIT unit Notes
STARTING WITH DNA
Full Service Sheared DNA (SPRIWORKS) $150 $225 per sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only $100 N/A per sample User must provide all primers. Samples will be returned for amplification.
Full Service Intact Genomic DNA (NEXTERA) $150 $225 per sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
STARTING WITH RNA
Illumina TruSeq $300 $450 per sample Preformed using TruSeq AND SPRIworks system.
Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+strand specific $30 $45 per sample Requires 1ug of RNA. Uses the dUTP method.
Epicenter Ribozero
no polyA pulldown
$350 $525 per sample Requires 1ug of RNA. Based on Illumina Truseq chemistry
Low Input (Clontech) $375 $575 per sample Can work on single cells
Low Input (NuGen) $400 $600 per sample Can work on degraded RNA.
Tight Size Cut $75 $125 per pool +/- 40bp using PippinPrep
STARTING WITH CHROMATIN Note: User must supply antibodies.
Full Service ChIP-seq $210 $315 per sample Preformed using IP-Star AND SPRIworks system.
Includes ChIP, library contruction and barcoding.
IP-Star Only $70 $105 per sample Preformed using IP-Star
qPCR of ChIP
in development
TBA TBA per sample Tests 8 IPs against 5 genomic loci in duplicate. User provides oligos.




.




MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS CORE LAB MIT Non-MIT unit Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng) $270 $360 $540 per sample Using NuGEN sample labeling kit.
Eukaryote (<20ng) $370 $460 $690 per sample Using NuGEN sample labeling kit.
Prokaryote $365 $455 $680 per sample Using Ambion sample labeling kit.
Fluidics Station and Scan $140 $165 N/A per array Users must be trained and coordinate with Manlin.
AGILENT PROCESSING
Expression - ONE COLOR - Eukaryote $180 $240 $360 per array
Expression - TWO COLOR - Eukaryote $250 $300 $450 per array Includes both Cy5 and Cy3
miRNA - ONE COLOR $230 $300 $450 per array
Prokaryote - ONE COLOR $230 $300 $450 per array
Scanning Only $50 $50 N/A per slide Price per glass slide.

prices effective 7.1.2012

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices
  • available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER CORE LAB / MIT Non-MIT unit Notes
High Sensitivity DNA or Nano RNA $10 $25 per sample
All other DNA and RNA samples $15 $25 per sample
High Sensitivity protein $40 $60 per sample detection of proteins at < 30ng/ul.
Flow Cytometry by request NA per sample

prices effective 7.1.2012
.

REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
FLUIDIGM
Fluidigm BioMark $150 $225 per use CALENDER Contact Shmulik
48.48 Dynamic Array Chip $260 N/A per cassette Cassettes are available in the BMC. Contact Shmulik
96.96 Dynamic Array Chip $760 N/A per cassette Cassettes are available in the BMC. Contact Shmulik
RT-PCR
Roche LightCycler 480 $10 NA per plate CALENDER *available for CORE only Contact Michael for training
MJ Opticon II $10 $15 per plate CALENDER Contact Manlin for training
SYBR Green (KAPA/Roche) $34 NA per ml Available for MIT only
96-well RTPCR PLATE $6 NA per plate Available for MIT only

prices effective 7.1.2012 .

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
PIPPIN PREP
Gel Isolation $50 $75 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Shmulik for training.
PLATE READER
Varioskan $10 $15 per session CALENDER Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Shmulik for training
ROBOTICS
Tecan EVO 150 $50 $150 per 2h block - based on scheduled time. CALENDER
non-filter tips $6.00 $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $100 $150 per session Contact Stuart
NANODROP
Nanodrop -- -- Walk up service * no charge

prices effective 7.1.2012

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES CORE LAB Other MIT / Non-MIT unit Notes
Informatics Support $65 $85 / $120 per hour Limited support for non-MIT users
Data storage (1 year) $400 N/A per TB (Limited space available)
Spotfire License (1 year) Contact Stephen Goldman N/A per user (Limited seats available)

FY2013 Pricing
FY2012 Pricing
FY2011 Pricing

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