BioMicroCenter:Pricing

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(ILLUMINA SEQUENCING)
(ILLUMINA SEQUENCING)
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  !width=100| unit
  !width=100| unit
  ! Notes
  ! Notes
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|-
 +
| colspan="5" align=center |<BR>'''GAII / GAIIx''' sequencing (minimum 10m reads/lane. Average ~25-30m reads/lane)
  |-
  |-
  | Single End (36nt)
  | Single End (36nt)
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  |align="center"| per lane
  |align="center"| per lane
  |   
  |   
 +
|-
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| colspan="5" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 25m reads/lane. Average ~75-100m reads/lane)
 +
|-
 +
| Single End (40nt)
 +
|align="right"| $1,270
 +
|align="right"| $1,905
 +
|align="center"| per lane
 +
| Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
 +
|-
 +
| Paired End (40+40nt)
 +
|align="right"| $2,230
 +
|align="right"| $3,345
 +
|align="center"| per lane
 +
|
 +
|-
 +
| Add'l 40nt
 +
|align="right"| $450
 +
|align="right"| $675
 +
|align="center"| per lane
 +
 +
|-
 +
| colspan="5" align=center |<BR>'''Additional Services'''
  |-
  |-
  | Sample Multiplexing
  | Sample Multiplexing
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  ! Notes
  ! Notes
  |-
  |-
-
  | Genomic DNA
+
  | Full Service Genomic DNA
-
  |align="right"| $225
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  |align="right"| $185
-
  |align="right"| $340
+
  |align="right"| $275
  |align="center"| per sample
  |align="center"| per sample
  | Includes adapter ligation, size selection, enrichment, and quality control (RT-PCR and BioAnalyzer),
  | Includes adapter ligation, size selection, enrichment, and quality control (RT-PCR and BioAnalyzer),
 +
|-
 +
| Robotics Only
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|align="right"| $130
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|align="right"| N/A
 +
|align="center"| per sample
 +
| User must provide all primers. Samples will be returned for amplification.
  |}
  |}
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Revision as of 14:42, 7 November 2010

Image:BioMicroCenter-header6.jpg

Services provided by the BioMicro Center are billed monthly to MIT account numbers (7-digit numbers). If you do not have an MIT account number, you will need to provide a PO number for our services. Cost estimates are available by request.

Contents

ILLUMINA SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer.

ILLUMINA SEQUENCING CORE LAB/MIT Non-MIT unit Notes

GAII / GAIIx sequencing (minimum 10m reads/lane. Average ~25-30m reads/lane)
Single End (36nt) $860 $1,290 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
Paired End (36+36nt) $1,500 $2,250 per lane
Add'l 36nt $300 $450 per lane

HiSEQ 2000 sequencing (minimum 25m reads/lane. Average ~75-100m reads/lane)
Single End (40nt) $1,270 $1,905 per lane Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
Paired End (40+40nt) $2,230 $3,345 per lane
Add'l 40nt $450 $675 per lane

Additional Services
Sample Multiplexing $50 $75 per 10 samples Combined over the whole order. Only applies to samples combined by BMC technicians.
Add'l Repriming $300 $450 per flow cell Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Quality Control Only $40 $60 per sample



Prices effective 7.1.2010

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.

ILLUMINA Sample Preparation CORE LAB/MIT Non-MIT unit Notes
Full Service Genomic DNA $185 $275 per sample Includes adapter ligation, size selection, enrichment, and quality control (RT-PCR and BioAnalyzer),
Robotics Only $130 N/A per sample User must provide all primers. Samples will be returned for amplification.





MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS CORE LAB MIT Non-MIT unit Notes
AFFYMETRIX PROCESSING
Eukaryote 3' (std) $280 $350 $525 per sample Using NuGEN sample labeling kit.
Eukaryote Exon $320 $420 $630 per sample Using NuGEN sample labeling kit.
Prokaryote $260 $360 $540 per sample Using Ambion sample labeling kit.
Fluidics Station and Scan $100 $125 N/A per array Users must be trained and coordinate with Manlin.
AGILENT PROCESSING
Expression - ONE COLOR $230 $315 $480 per array
Expression and CGH - TWO COLOR $335 $420 $630 per array Includes both Cy5 and Cy3
miRNA - ONE COLOR $250 $295 $450 per array
Scanning Only $50 $50 N/A per slide Price per glass slide.

prices effective 7.1.2010

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices

Common Agilent Expression Arrays*^:

$807.20  - 8x15k designs (cost per tile, must use all 8 at once)       E.coli / S.cerevisiae / Custom
$170     - 4x44k designs (cost per tile, must use all 4 at once)       Human / Mouse / C.elegans / Custom


  • available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

^ Discount pricing available through the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER CORE LAB / MIT Non-MIT unit Notes
All DNA and RNA samples $15 $25 per sample
High Sensitivity protein $40 $60 per sample detection of proteins at < 30ng/ul.
Flow Cytometry by request NA per sample

prices effective 7.1.2010
.

REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
FLUIDIGM
Fluidigm BioMark $100 $150 per cassette Cassettes will be available in BMC soon. Contact Manlin
RT-PCR
Roche LightCycler 480 $10 NA per plate CALENDER *available for CORE only Contact Allison for training
MJ Opticon II $10 $15 per plate CALENDER Contact Manlin for training
SYBR Green (Roche) $40 NA per ml Available for MIT only
96-well RTPCR PLATE $6 NA per plate Available for MIT only

prices effective 7.1.2010 .

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
PLATE READER
Varioskan $10 $15 per plate CALENDER Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Kevin for training
ROBOTICS
Tecan EVO 150 $75 $125 per session CALENDER (cost may decrease depending on usage levels)
non-filter tips $5.00 $5.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $100 $150 per session Contact Stuart Levine
NANOSTRING
Nanostring $10.00 N/A per sample Contact Manlin Luo ** available on a trial basis
NANODROP
Nanodrop -- -- Walk up service * no charge

prices effective 7.1.2010

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES CORE LAB Other MIT / Non-MIT unit Notes
Data storage (1 year) $500 N/A per TB (Limited space available)
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