BioMicroCenter:Pricing

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(ILLUMINA SEQUENCING)
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{{BioMicroCenter}}
{{BioMicroCenter}}
-
== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==
 
-
 
-
 
=== SEQUENCING ===
=== SEQUENCING ===
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq.
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq.
Line 18: Line 15:
  | Single End (40nt)
  | Single End (40nt)
  |align="right"| $1,085
  |align="right"| $1,085
-
  |align="right"| $1,195
+
  |align="right"| $1,194
-
  |align="right"| $1,628
+
  |align="right"| $1,778
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
  |-
  |-
  | Paired End (40+40nt)
  | Paired End (40+40nt)
Line 36: Line 33:
  |align="center"| per lane
  |align="center"| per lane
  |
  |
 +
|-
 +
| colspan="6" align=center |<BR>'''NextSeq''' sequencing (minimum 250m reads/lane. maximum ~500m reads/lane)
 +
|-
 +
| 91nt kit <BR> (eg. 40PE or 50SE)
 +
|align="right"| $2,000
 +
|align="right"| $2,200
 +
|align="right"| $3,000
 +
|align="center"| per flowcell
 +
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year <BR> ** THESE RUNS WILL HAVE LOW PRIORITY
 +
|-
 +
| 166nt kit <BR> (eg. 80PE or 150SE)
 +
|align="right"| $3,000
 +
|align="right"| $3,300
 +
|align="right"| $4,500
 +
|align="center"| per flowcell
 +
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 +
|-
 +
| 316nt kit <BR> (eg. 150PE or 300SE)
 +
|align="right"| $4,500
 +
|align="right"| $4,950
 +
|align="right"| $6,750
 +
|align="center"| per flowcell
 +
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-  
  |-  
  | colspan="6" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
  | colspan="6" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
  |-
  |-
-
  | 70nt Run
+
  | 70nt Run <BR> (eg. 30PE or 70SE)
-
  |align="right"| $1,150
+
  |align="right"| $1,100
-
  |align="right"| $1,265
+
  |align="right"| $1,210
-
  |align="right"| $1,725
+
  |align="right"| $1,650
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
-
  | 300nt Run (PE or SE)
+
  | 300nt Run <BR> (eg. 150PE)
-
  |align="right"| $1,350
+
  |align="right"| $1,300
-
  |align="right"| $1,485
+
  |align="right"| $1,430
-
  |align="right"| $2,025
+
  |align="right"| $1,950
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
-
  | 500nt Run (PE or SE)
+
  | 500nt Run <BR> (eg. 250PE)
-
  |align="right"| $1,600
+
  |align="right"| $1,500
-
  |align="right"| $1,760
+
  |align="right"| $1,650
-
  |align="right"| $2,400
+
  |align="right"| $2,250
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
  | colspan="6" align=center |<BR>'''MiSeq v3''' sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
  | colspan="6" align=center |<BR>'''MiSeq v3''' sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
  |-
  |-
-
  | 600nt Run (PE or SE)
+
  | 150nt Run <BR> (eg. 75PE)
-
  |align="right"| $2,050
+
  |align="right"| $1,300
-
  |align="right"| $2,255
+
  |align="right"| $1,430
-
  |align="right"| $3,075
+
  |align="right"| $1,950
  |align="center"| per lane
  |align="center"| per lane
-
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
  |-
  |-
  | colspan="6" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
  | colspan="6" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
Line 82: Line 102:
  |align="right"| $75
  |align="right"| $75
  |align="center"| per 10 samples
  |align="center"| per 10 samples
-
  | Combined over the whole order. Only applies to samples combined by BMC technicians.
+
  | Combined over the whole order. Only applies to samples combined by BMC technicians.<BR>Waived for libraries created by the BMC.
  |-
  |-
-
  | Add'l Repriming (HiSeq only)
+
  | Dual Index read (HiSeq only)
  |align="right"| $200
  |align="right"| $200
  |align="right"| $220
  |align="right"| $220
  |align="right"| $300
  |align="right"| $300
  |align="center"| per flow cell
  |align="center"| per flow cell
-
  | Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
+
  | Uses 6-8nt. Cost is divided by number of lanes requiring dual indexing. Initial index read is included.  
  |-
  |-
-
  | Quality Control Only
+
  | Quality Control Only -only one QC is included per sequencing lane.
-
  |align="right"| $25
+
  |align="right"| $20
-
  |align="right"| $28
+
  |align="right"| $26
-
  |align="right"| $38
+
  |align="right"| $30
  |align="center"| per sample
  |align="center"| per sample
  | Includes RT-PCR and BioAnalyzer
  | Includes RT-PCR and BioAnalyzer
Line 101: Line 121:
<BR><BR>
<BR><BR>
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR>
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR>
-
 
=== SAMPLE PREPARATION ===
=== SAMPLE PREPARATION ===
Line 116: Line 135:
  |-
  |-
  | Full Service Sheared DNA (SPRIWORKS)
  | Full Service Sheared DNA (SPRIWORKS)
-
  |align="right"| $150
+
  |align="right"| $130
-
  |align="right"| $165
+
  |align="right"| $143
-
  |align="right"| $225
+
  |align="right"| $195
  |align="center"| per sample
  |align="center"| per sample
  | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
  | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
  |-
  |-
  | ''SPRI-TE use only''
  | ''SPRI-TE use only''
-
  |align="right"| $100
+
  |align="right"| $85
-
  |align="right"| $110
+
  |align="right"| $95
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per sample
  |align="center"| per sample
Line 130: Line 149:
  |-
  |-
  | Intact Genomic DNA (NEXTERA) - 50ng input
  | Intact Genomic DNA (NEXTERA) - 50ng input
-
  |align="right"| $175
+
  |align="right"| $150
-
  |align="right"| $193
+
  |align="right"| $165
-
  |align="right"| $263
+
  |align="right"| $225
  |align="center"| per sample
  |align="center"| per sample
  | Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
  | Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
Line 142: Line 161:
  |align="center"| per sample
  |align="center"| per sample
  | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).  
  | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).  
 +
|-
 +
| AUTOMATED NEXTERA-XT - 1ng input
 +
|align="right"| $1000
 +
|align="right"| $1100
 +
|align="right"| $1500
 +
|align="center"| per 16 samples
 +
| Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). May have 10% failure rate.
  |-
  |-
  | 16S Amplicon
  | 16S Amplicon
-
|align="right"| $1,200
 
-
|align="right"| $1,320
 
  |align="right"| $1,800
  |align="right"| $1,800
 +
|align="right"| $1,980
 +
|align="right"| $2,700
  |align="center"| per 48 sample
  |align="center"| per 48 sample
  | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).  
  | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).  
  |-
  |-
  | Other Amplicon
  | Other Amplicon
-
|align="right"| $960
 
-
|align="right"| $1,056
 
  |align="right"| $1,440
  |align="right"| $1,440
 +
|align="right"| $1,584
 +
|align="right"| $2,160
  |align="center"| per 48 sample
  |align="center"| per 48 sample
  | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). <BR> Samples *must* be submitted amplified with appropriate linkers.
  | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). <BR> Samples *must* be submitted amplified with appropriate linkers.
Line 159: Line 185:
  | colspan="6" | '''STARTING WITH RNA'''  
  | colspan="6" | '''STARTING WITH RNA'''  
  |-
  |-
-
  | Illumina TruSeq  
+
  | Illumina TruSeq (stranded or non)
-
|align="right"| $300
+
  |align="right"| $275
-
|align="right"| $330
+
  |align="right"| $302.5
-
|align="right"| $450
+
  |align="right"| $412.5
-
|align="center"| per sample
+
-
| Preformed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+
-
|-
+
-
|''+strand specific''
+
-
  |align="right"| $30
+
-
  |align="right"| $33
+
-
  |align="right"| $45
+
  |align="center"| per sample
  |align="center"| per sample
-
  | Requires 1ug of RNA. Uses the dUTP method.
+
  | Performed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Strand specifc by dUTP method
  |-
  |-
  | Epicenter Ribozero<br>''no polyA pulldown''
  | Epicenter Ribozero<br>''no polyA pulldown''
Line 181: Line 200:
  |-
  |-
  | Low Input (Clontech)
  | Low Input (Clontech)
-
  |align="right"| $400
+
  |align="right"| $375
-
  |align="right"| $440
+
  |align="right"| $412.5
-
  |align="right"| $600
+
  |align="right"| $562.5
  |align="center"| per sample
  |align="center"| per sample
  | Can work on single cells
  | Can work on single cells
  |-
  |-
  | Low Input (NuGen)
  | Low Input (NuGen)
-
  |align="right"| $475
+
  |align="right"| $430
-
  |align="right"| $523
+
  |align="right"| $473
-
  |align="right"| $713
+
  |align="right"| $645
  |align="center"| per sample
  |align="center"| per sample
  | Can work on degraded RNA.
  | Can work on degraded RNA.
  |-
  |-
  | Tight Size Cut
  | Tight Size Cut
-
  |align="right"| $100
+
  |align="right"| $85
-
  |align="right"| $110
+
  |align="right"| $94
-
  |align="right"| $150
+
  |align="right"| $130
  |align="center"| per pool
  |align="center"| per pool
  | +/- 40bp using PippinPrep
  | +/- 40bp using PippinPrep
Line 215: Line 234:
  |align="right"| $315
  |align="right"| $315
  |align="center"| per sample
  |align="center"| per sample
-
  | Preformed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
+
  | Performed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
  |-
  |-
  | ''IP-Star Only''
  | ''IP-Star Only''
Line 222: Line 241:
  |align="right"| $450
  |align="right"| $450
  |align="center"| per batch (1-8 samples)
  |align="center"| per batch (1-8 samples)
-
  | Preformed using IP-Star
+
  | Performed using IP-Star
 +
|-
 +
| Covaris Sonicator
 +
|align="right"| $10
 +
|align="right"| $15
 +
|align="right"| NA
 +
|align="center"| per hour
 +
| Training Required
  |-
  |-
|}
|}
Line 247: Line 273:
  |-
  |-
  | Eukaryote (3' or Exon: >20ng)
  | Eukaryote (3' or Exon: >20ng)
-
  |align="right"| $300
+
  |align="right"| $200
-
  |align="right"| $380
+
  |align="right"| $250
-
  |align="right"| $570
+
  |align="right"| $325
  |align="center"| per sample
  |align="center"| per sample
-
  | Using NuGEN sample labeling kit.
+
  | Using AFFYMETRIX sample labeling kit. For NuGEN reagents, please refer to 2015 pricing.
  |-
  |-
  | Eukaryote (<20ng)
  | Eukaryote (<20ng)
Line 269: Line 295:
  | colspan="6" | '''AGILENT PROCESSING'''
  | colspan="6" | '''AGILENT PROCESSING'''
  |-
  |-
-
  | Expression - ONE COLOR - Eukaryote
+
  | colspan="6" align=center |Agilent array services are being phased out in FY2016. Requests that must be processed on Agilent will be priced per experiment.
-
|align="right"| $200
+
-
|align="right"| $250
+
-
|align="right"| $375
+
-
|align="center"| per array
+
-
|
+
-
|-
+
-
| Expression - TWO COLOR - Eukaryote
+
-
|align="right"| $290
+
-
|align="right"| $340
+
-
|align="right"| $510
+
-
|align="center"| per array
+
-
| Includes both Cy5 and Cy3
+
-
|-
+
-
| miRNA - ONE COLOR
+
-
|align="right"| $260
+
-
|align="right"| $320
+
-
|align="right"| $480
+
-
|align="center"| per array
+
-
|
+
-
|-
+
-
| Prokaryote - ONE COLOR
+
-
|align="right"| $200
+
-
|align="right"| $260
+
-
|align="right"| $390
+
-
|align="center"| per array
+
-
|
+
  |-  
  |-  
  | Scanning Only
  | Scanning Only
Line 305: Line 305:
  |-  
  |-  
|}
|}
-
prices effective 7.1.2013
+
prices effective 7.1.2015
<br>
<br>
Line 314: Line 314:
  $400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
  $400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
  Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
  Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
-
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.  
+
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
Line 332: Line 332:
  | AA - Preloaded plate
  | AA - Preloaded plate
  |align="right"| $30
  |align="right"| $30
-
  |align="right"|  
+
  |align="right"| N/A
  |align="center"| per row (12)
  |align="center"| per row (12)
  | 2ul per sample in each well using plates from BMC
  | 2ul per sample in each well using plates from BMC
Line 349: Line 349:
  |-
  |-
|}
|}
-
prices effective 7.1.2012
+
prices effective 7.1.2015
<br>.
<br>.
Line 369: Line 369:
  |align="center"| per use
  |align="center"| per use
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
-
  | Contact [[BioMicroCenter:People|Shmulik]]  
+
  | Contact [[BioMicroCenter:People|Jon Penterman]]  
  |-
  |-
  | 48.48 Dynamic Array Chip
  | 48.48 Dynamic Array Chip
Line 379: Line 379:
  |-
  |-
  | 96.96 Dynamic Array Chip
  | 96.96 Dynamic Array Chip
-
  |align="right"| $700
+
  |align="right"| $825
  |align="right"| N/A
  |align="right"| N/A
  |align="center"| per cassette
  |align="center"| per cassette
  | Cassettes are available in the BMC.
  | Cassettes are available in the BMC.
  |
  |
-
|-
 
-
| WAFERGEN SMART CHIP
 
-
|align="right"| $150
 
-
|align="right"| N/A
 
-
|align="center"| per chip
 
-
| Chips available from BMC
 
-
| Contact [[BioMicroCenter:People|Michael]]
 
-
|-
 
-
| WAFERGEN TRAINING
 
-
|align="right"| $100
 
-
|align="right"| N/A
 
-
|align="center"| per chip
 
-
| Mandatory
 
-
| Contact [[BioMicroCenter:People|Michael]]
 
-
|- | colspan="6" align="center"| '''RT-PCR'''
 
  |-
  |-
  | Roche LightCycler 480
  | Roche LightCycler 480
  |align="right"| $10
  |align="right"| $10
-
  |align="right"| NA
+
  |align="right"| N/A
  |align="center"| per plate
  |align="center"| per plate
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
-
  | Contact [[BioMicroCenter:People|Scott]] for training
+
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training
  |-
  |-
  | SYBR Green (KAPA/Roche)
  | SYBR Green (KAPA/Roche)
  |align="right"| $35
  |align="right"| $35
-
  |align="right"| NA
+
  |align="right"| N/A
  |align="center"| per ml
  |align="center"| per ml
  |
  |
Line 416: Line 401:
  | 96-well PLATES
  | 96-well PLATES
  |align="right"| $6
  |align="right"| $6
-
  |align="right"| NA
+
  |align="right"| N/A
  |align="center"| per plate
  |align="center"| per plate
  |
  |
Line 422: Line 407:
  |-
  |-
|}
|}
-
prices effective 7.1.2013
+
prices effective 7.1.2014
.
.
<br>
<br>
Line 450: Line 435:
  |align="center"| per session
  |align="center"| per session
  | TBA
  | TBA
-
  | Contact [[BioMicroCenter:People|Shmulik]] for training.
+
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training.
  |-
  |-
| colspan="6" align="center"| '''PLATE READER'''
| colspan="6" align="center"| '''PLATE READER'''
  |-
  |-
  | Varioskan
  | Varioskan
-
  |align="right"| $10
+
  |align="right"| $12
-
  |align="right"| $15
+
  |align="right"| $18
-
  |align="center"| per session
+
  |align="center"| per hour
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
-
  | Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Shmulik]] for training  
+
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training  
  |-
  |-
  | colspan="6" align="center"| '''ROBOTICS'''
  | colspan="6" align="center"| '''ROBOTICS'''
Line 501: Line 486:
  |-
  |-
|}
|}
-
prices effective 7.1.2013
+
prices effective 7.1.2014
<br><br>
<br><br>
Line 526: Line 511:
  | Data storage (1 year)
  | Data storage (1 year)
  |align="right"| $280
  |align="right"| $280
-
  |align="right"| N/A
+
  |align="right"| $400 / N/A
  |align="center"| per TB
  |align="center"| per TB
  |  
  |  
  |-
  |-
-
  | ROUS node (1 year)
+
  | Data storage (1 year)
-
  |align="right"| $400
+
  |align="right"| $280
-
  |align="right"| N/A
+
  |align="right"| $400 / N/A
-
  |align="center"| 8 CPU
+
  |align="center"| per TB
-
  | Provides a priority queue for the lab on ROUS.
+
  |  
-
  |-  
+
  |-
-
  | Spotfire License (1 year)
+
  | Tape Archive / Recovery
-
  |align="right"| ''Contact Stephen Goldman''
+
  |align="right"| $60
-
  |align="right"| N/A
+
  |align="right"| $70
-
  |align="center"| per user
+
  |align="center"| per tape
-
  | (Limited seats available)
+
  |  
  |-
  |-
|}
|}
 +
 +
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]]<BR>
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]]<BR>
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Current revision

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Contents

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the HiSeq.

ILLUMINA SEQUENCING CORE LAB MIT Non-MIT unit Notes

HiSEQ 2000 sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
Single End (40nt) $1,085 $1,194 $1,778 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Paired End (40+40nt) $1,625 $1,788 $2,438 per lane
Add'l 40nt $300 $330 $450 per lane

NextSeq sequencing (minimum 250m reads/lane. maximum ~500m reads/lane)
91nt kit
(eg. 40PE or 50SE)
$2,000 $2,200 $3,000 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
** THESE RUNS WILL HAVE LOW PRIORITY
166nt kit
(eg. 80PE or 150SE)
$3,000 $3,300 $4,500 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
316nt kit
(eg. 150PE or 300SE)
$4,500 $4,950 $6,750 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

MiSeq v2 sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
70nt Run
(eg. 30PE or 70SE)
$1,100 $1,210 $1,650 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
300nt Run
(eg. 150PE)
$1,300 $1,430 $1,950 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
500nt Run
(eg. 250PE)
$1,500 $1,650 $2,250 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

MiSeq v3 sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
150nt Run
(eg. 75PE)
$1,300 $1,430 $1,950 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

GAII / GAIIx sequencing (Average ~25-40m reads/lane)
GAII Services are being phased out in FY2015. Requests that must be processed on the GAII will be priced per experiment.

Additional Services
These services apply to samples submitted for all platforms.
Sample Multiplexing $50 $55 $75 per 10 samples Combined over the whole order. Only applies to samples combined by BMC technicians.
Waived for libraries created by the BMC.
Dual Index read (HiSeq only) $200 $220 $300 per flow cell Uses 6-8nt. Cost is divided by number of lanes requiring dual indexing. Initial index read is included.
Quality Control Only -only one QC is included per sequencing lane. $20 $26 $30 per sample Includes RT-PCR and BioAnalyzer



Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SAMPLE PREPARATION

ILLUMINA Sample Preparation CORE LAB MIT Non-MIT unit Notes
STARTING WITH DNA
Full Service Sheared DNA (SPRIWORKS) $130 $143 $195 per sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only $85 $95 N/A per sample User must provide all primers. Samples will be returned for amplification.
Intact Genomic DNA (NEXTERA) - 50ng input $150 $165 $225 per sample Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input $100 $110 $150 per sample Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
AUTOMATED NEXTERA-XT - 1ng input $1000 $1100 $1500 per 16 samples Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). May have 10% failure rate.
16S Amplicon $1,800 $1,980 $2,700 per 48 sample Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
Other Amplicon $1,440 $1,584 $2,160 per 48 sample Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
Samples *must* be submitted amplified with appropriate linkers.
STARTING WITH RNA
Illumina TruSeq (stranded or non) $275 $302.5 $412.5 per sample Performed using TruSeq AND SPRIworks system.
Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Strand specifc by dUTP method
Epicenter Ribozero
no polyA pulldown
$350 $385 $525 per sample Requires 1ug of RNA. Based on Illumina Truseq chemistry
Low Input (Clontech) $375 $412.5 $562.5 per sample Can work on single cells
Low Input (NuGen) $430 $473 $645 per sample Can work on degraded RNA.
Tight Size Cut $85 $94 $130 per pool +/- 40bp using PippinPrep
Small RNA $275 $303 $413 per sample Uses NEB small RNA kit
STARTING WITH CHROMATIN Note: User must supply antibodies.
Full Service ChIP-seq $210 $231 $315 per sample Performed using IP-Star AND SPRIworks system.
Includes ChIP, library contruction and barcoding.
IP-Star Only $300 $330 $450 per batch (1-8 samples) Performed using IP-Star
Covaris Sonicator $10 $15 NA per hour Training Required

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MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS CORE LAB MIT Non-MIT unit Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng) $200 $250 $325 per sample Using AFFYMETRIX sample labeling kit. For NuGEN reagents, please refer to 2015 pricing.
Eukaryote (<20ng) $400 $500 $750 per sample Using NuGEN sample labeling kit.
Prokaryote $365 $455 $683 per sample Using Ambion sample labeling kit.
AGILENT PROCESSING
Agilent array services are being phased out in FY2016. Requests that must be processed on Agilent will be priced per experiment.
Scanning Only $50 $50 N/A per slide Price per glass slide.

prices effective 7.1.2015

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices
  • available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER CORE LAB / MIT Non-MIT unit Notes
RNA / DNA on Advanced Analytical $8 $12 per sample
AA - Preloaded plate $30 N/A per row (12) 2ul per sample in each well using plates from BMC
All DNA & RNA on BIOANALYZER $15 $25 per sample
High Sensitivity protein $40 $60 per sample detection of proteins at < 30ng/ul.

prices effective 7.1.2015
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REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
FLUIDIGM
Fluidigm BioMark $150 $225 per use CALENDER Contact Jon Penterman
48.48 Dynamic Array Chip $250 N/A per cassette Cassettes are available in the BMC.
96.96 Dynamic Array Chip $825 N/A per cassette Cassettes are available in the BMC.
Roche LightCycler 480 $10 N/A per plate CALENDER *available for CORE only Contact Jon Penterman for training
SYBR Green (KAPA/Roche) $35 N/A per ml Available for MIT only
96-well PLATES $6 N/A per plate Available for MIT only

prices effective 7.1.2014 .

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
PIPPIN PREP
Gel Isolation $50 $75 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Jon Penterman for training.
PLATE READER
Varioskan $12 $18 per hour CALENDER Contact Jon Penterman for training
ROBOTICS
Tecan EVO 150 $60 $150 per 2h block - based on scheduled time. CALENDER
non-filter tips $6.00 $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $100 $150 per session Contact Stuart
NANODROP
Nanodrop -- -- Walk up service * no charge

prices effective 7.1.2014

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES CORE LAB Other MIT / Non-MIT unit Notes
Informatics Support $70 $90 / $135 per hour Limited support for non-MIT users
Informatics Share $960 N/A per 0.1FTE MIT only. Priority access to informatics.
Data storage (1 year) $280 $400 / N/A per TB
Data storage (1 year) $280 $400 / N/A per TB
Tape Archive / Recovery $60 $70 per tape


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