BioMicroCenter:News: Difference between revisions
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== BioMicro Center News == | == BioMicro Center News == | ||
=== AUGUST 2014 === | |||
We have set up a collaboration with the University of Massachusetts Medical Center Deep Sequencing Core Facility to provide Pacific Biosciences seqeucning to MIT users. We will be providing initial library preparation and quality control before sending the samples out to Worcester for sequencing. Completed sequence data will be returned through your BioMicro Center public folders. More details about PacBio sequencing can be found on our website.<BR><BR> | |||
The Illumina pipeline has been upgraded to version 1.3. This upgrade includes quality control metrics for RNAseq and paired end reads as well as some changes under the hood to speed up data delivery. These quality control metrics are dependant on genome alignment, so will not be produced for samples aligned to phiX.<BR><BR> | |||
Most of the price increases scheduled for the next fiscal year have been cancelled. We will be re-evaluating the rates in 6 month increments to ensure we remain within our mandated budget constraints.<BR><BR> | |||
Our new co-op students, Austin Hendricks and Adam Perez have joined us and will be working in the Center through December. | |||
The following packages have been added or updated on ROUS in the past few months: | |||
* trinityrnaseq: r20140413p1 | |||
* bedtools: 2.20.1 | |||
* bowtie2: 2.2.3 | |||
* tophat: 2.0.12 | |||
* rsem: 1.2.15 | |||
* fastqc: 0.11.2 | |||
* bwa: 0.7.10 | |||
* pear: 0.9.4 | |||
* perl: 5.20.0 | |||
* samtools: 1.0 | |||
Older versions can still be selected using the module function. We’ve also created a new mailing list rous_active_users@mit.edu to help keep folks who use Rous regularly up to date. You can sign up for it using Moira. | |||
=== MAY 2014 === | |||
Some quick bullet pointed updates from BioMicro. We’re in the middle of rolling out several new initiatives right now that will hopefully be in the July newsletter. | |||
* We are now offering sample prep using the [[BioMicroCenter:Nextera|Nextera XT]] system from Illumina. This modification of the Nextera uses only 1ng of input DNA (instead of the typical 50ng) and is significantly less expensive. Tuning it for insert size is somewhat more challenging than standard Nextera and so it should not be used where insert size is critical. | |||
* [[BioMicroCenter:PricingFY2015|Pricing for 2015]] is up on our website. There is a link at the bottom of the pricing page. | |||
* The Wafergen system is no longer available in the BioMicro Center. There is a system at Children’s Hospital if you absolutely need the Wafergen system. Most applications can also be done on the [[BioMicroCenter:RTPCR|Fluidigm BioMark]], which we still have in the lab. | |||
* This month, we say goodbye to Scott Morin who will be leaving for medical school. Leigh Manley, who has transferred over from the Biopolymer core, will be taking over Illumina processing. | |||
* Finally, the BioMicro Center will be shifting over to iLabs in the coming months. We are still early in the process but we would encourage everyone to [https://mit-ki.ilabsolutions.com/account/login register for iLabs] to help smooth the transition. | |||
=== MARCH 2014 === | |||
A couple highlights of things going on in the center. | |||
* We are resuming the [[BioMicroCenter:Technology_Seminar_Series|Technology Seminar Series]] this week with Roche speaking on Wednesday at lunch. The talk will focus on non-standard applications of the Light Cycler (SNP detection, etc). | |||
* The Fraenkel lab has very kindly donated their [[BioMicroCenter:Covaris|Covaris sonicator]] to the core and we will be putting it under a service contract. We will need to put in place a small fee to cover the cost of the contract - likely starting in July. Training for new users will be available through [[BioMicroCetner:People|Shmulik]]. | |||
* We’ve generally had positive feedback about our new AA/BioA form and we’re expanding the new excel forms to include Illumina sequencing. [[BioMicroCenter:Forms|This new form will cover most types of sample preparation.]] | |||
* Illumina is raising the prices on all of their sequencing reagents starting April 1. We lowered our prices very aggressively last year so, in order to break even, [[BioMicroCenter:Pricing#SEQUENCING|we will have adjust our prices slightly higher.]] | |||
=== JANUARY, 2014 === | === JANUARY, 2014 === | ||
I hope everyone had a fantastic holiday. We have several updates to let you know about. Since there are a few more than usual, I’m just going to give you the bullet list which you can check out on our website. | I hope everyone had a fantastic holiday. We have several updates to let you know about. Since there are a few more than usual, I’m just going to give you the bullet list which you can check out on our website. | ||
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== PUBLICATIONS == | == PUBLICATIONS == | ||
'''2014'''<BR><BR> | '''2014'''<BR><BR> | ||
<biblio> | <biblio> | ||
#Paper1 pmid=24501120 <!- RPA Walker-> | #Paper1 pmid=24501120 <!- RPA Walker-> | ||
#Paper2 pmid=24501121 <!- RPA Walker-> | #Paper2 pmid=24501121 <!- RPA Walker-> | ||
#Paper3 pmid=24249727 <!- VB Saeij-> | |||
#Paper4 pmid=24757057 <!- RPA.VB Samson-> | |||
#Paper5 pmid=24763590 <!- HD Chisholm-> | |||
#Paper6 pmid=24899568 <!- VB.SL Dedon-> | |||
#Paper7 pmid=24931974 <!- VB Burge-> | |||
#Paper8 pmid=24413286 <!- RPA.SL Tannenbaum Fox-> | |||
</biblio> | |||
'''2013'''<BR><BR> | '''2013'''<BR><BR> | ||
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#Paper7 pmid=24009526 <!- CW Lees-> | #Paper7 pmid=24009526 <!- CW Lees-> | ||
#Paper8 pmid=23873940 <!- CW Jacks2-> | #Paper8 pmid=23873940 <!- CW Jacks2-> | ||
#Paper10 pmid=24134150 <!- SL.RPA Tannenbaum-> | #Paper10 pmid=24134150 <!- SL.RPA Tannenbaum-> | ||
#Paper11 pmid=24367253 <!- VB Saeij-> | #Paper11 pmid=24367253 <!- VB Saeij-> | ||
#Paper12 pmid=23703590 <!- SM Fraenkel -> | |||
</biblio> | </biblio> | ||
'''2012'''<BR><BR> | '''2012'''<BR><BR> |
Revision as of 07:31, 19 August 2014
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Welcome to the MIT BIOMICRO CENTER
BioMicro Center NewsAUGUST 2014We have set up a collaboration with the University of Massachusetts Medical Center Deep Sequencing Core Facility to provide Pacific Biosciences seqeucning to MIT users. We will be providing initial library preparation and quality control before sending the samples out to Worcester for sequencing. Completed sequence data will be returned through your BioMicro Center public folders. More details about PacBio sequencing can be found on our website.
Older versions can still be selected using the module function. We’ve also created a new mailing list rous_active_users@mit.edu to help keep folks who use Rous regularly up to date. You can sign up for it using Moira. MAY 2014Some quick bullet pointed updates from BioMicro. We’re in the middle of rolling out several new initiatives right now that will hopefully be in the July newsletter.
MARCH 2014A couple highlights of things going on in the center.
JANUARY, 2014I hope everyone had a fantastic holiday. We have several updates to let you know about. Since there are a few more than usual, I’m just going to give you the bullet list which you can check out on our website.
SEPTEMBER 12, 2013I hope everyone had a great summer. A couple new things to tell you about with the start of the academic year. First, we have two new bioinformatics scientists on board. Dr. Duan Ma comes to us from the Dept of Biostatistics at Washington University as well as several years in industry where she worked on a broad variety of bioinformatics problems as well as cloud computing solutions. Dr. Jie Wu did his PhD in computational biology Cold Spring Harbor where he focused on developing algorithms for RNAseq. Both Jie and Duan are located in 68-317, so please stop by and say hello. One new product to tell you about is new MiSeq v3 kits which just came out. These kits increase read counts to 25m from the current 15m maximum. The kits only come in 150 and 600 nt sizes and are somewhat more expensive than the current reagents. The changes to the MiSeq do not help v2 kits and they cannot reach the 25m threshold. We will be offering both the v2 and v3 kits for a short while, but our expectation is the v2 kits will be discontinued by Illumina in the not so distant future. New pricing for the v3 kits is up on our BioMicroCenter:Pricing pricing site. Also on the sequencing front, we have some evidence that the CG bias issues we were seeing on the HiSeq may not have been totally resolved. We are in the middle of implementing some significant changes in our control lane chemistry in collaboration with Illumina. These will at least allow us to monitor the situation very aggressively and detect any changes in GC bias by using a more complex mixture of samples, instead of only phiX. As I mentioned in our April newsletter, if you have reason to believe your data was compromised by this issue, please come talk to us so we can help get replacement kits to get your samples rerun as soon as possible. The new control lane is in early beta but is being included on all HiSeq flowcells now. We do want to thank the Laub, Niles and Walker labs for DNA to help us in this effort. Finally, the Technology Seminar Series will return this year. We begin next Wednesday with Wafergen talking about the Smartchip system. The seminar will be at noon in 68-180 and lunch will be provided. We’re hoping to have seminars every month throughout the year so be sure to look for them on our website. JUNE 17, 2013First, we have a number of BioMicroCenter:People staffing changes to tell you about. The end of June will see three members of the core moving on. Ryan Abo, one of our informatics scientists, will be heading to the Dana Farber and Paraj Patel and Pierrick Millet will be returning to Northeastern. Our new co-op students, Ashley Machado and Alexander Soltoff will be starting July 1st. We are currently undertaking a job search to look for Ryan’s replacement. If you have any concerns about the personnel changes, please feel free to contact me. In a piece of good news, the root cause of the critical failures we have had with our MiSeq for the last month plus with homopolymer samples appear to have been identified and should be fixed today. A recent software upgrade that was supposed to improve the handling of homopolymeric samples apparently failed to install properly, resulting in a mix of pipeline versions that was unable to handle the sequences at all. I do want to thank everyone for their patience as we have struggled with this problem and assure everyone we will move through our backlog on the MiSeq as fast as we possibly can. I also want to thank the techs in the lab, especially Scott Morin, who have been working weekends to try to get as many samples through the MiSeq as possible. Finally, with the end of the fiscal year, our annual price adjustments are due to take effect on July 1st. You can find a complete list of the new prices here . The largest change is a reduction in cost for HiSeq sequencing, especially for longer reads. This is associated with a significant decrease in the amount of time we will be holding data on our servers and with the recent switch from fastq + SAM file formats to retaining only BAM files. (see January 2013 notes).
APRIL 20, 2013We have noticed a number of technical issues with some Illumina runs. We want to share with you to make sure you are aware of some changes and newly identified technical issues with the platform and what we are doing to correct them where we can. All of these changes are from the Illumina side and none were especially well documented (some not at all). These issues are unlikely to be limited to the BMC, so samples from elsewhere on campus or around the country may also have these issues. Please read this as it may have some impact on your analyses. Just to begin, all of these changes are subtle and not obvious in most cases directly from the sequencers. It was the rare cases that had dramatic effects that caused us to notice them. If you decide you need to have samples rerun, we will work with you to try to get Illumina to replace the reagents and to get the samples rerun. Unfortunately, there is no way we can possibly do bulk reruns of several months’ worth of studies. The most concerning issue is a dropout of GC rich regions in clustering. This has been an on-again off-again issue with Illumina that we have addressed over a year ago by improvements in amplification cycling conditions and enzyme selection. Some time, several months ago (we do not have a precise window), Illumina appears to have changed the chemistry of one of their clustering components and that caused a major change in performance on GC rich areas. This can be seen as an absence of reads from very GC rich areas but, because these areas are rare in most genomes, they cannot be seen on the flowcell wide metrics. This issue is found on current HiSeq and MiSeqV2 kits but not on MiSeqV1 kits nor, we suspect, on the GAII. We have been able to address this problem by adding a brief boiling step during NaOH denaturation of the samples and have implemented this as SOP starting about two weeks ago. This drop out of regions can cause significant issues for several studies – most notably ChIP analyses – when you are comparing data from different chemistries. A second concern is one that has been reported in the community but we have not identified on our machines – yet – where samples from a run are being observed in the following run as minor contaminants. This issue is limited to the MiSeq and HiSeq2500 (we do not have the latter) where the tubes that add sample to the flowcell are not changed. This contamination is reported to be <1% and so would not show up on our quality metrics. However, if your MiSeq analyses are being based on finding a few reads in a large pool of discarded data or you are doing a number of sequential runs, you may wish to validate your data more carefully using an alternative technique such as qPCR or sanger sequencing. There is currently no technical fix to this problem. A third issue has been around for a while though we had not appreciated the implications. Illumina’s newer versions of basecalling software have become less capable of handling uniform sequence (all A’s for example). In earlier versions, only 5 basepairs of variability were needed and intensities could be determined by the control lane we run on all HiSeq flowcells. Now, it appears that nt 1-25 all must have representation of all 4 bases at all positions, even with a control lane. This has always been an issue on the MISeq and we have solved it by spiking in 30%PhiX in the lane (as opposed to our normal 0.1% spike in). Similar solutions can be used on the HiSeq. Given this change, we are re-evaluating whether there is value in using the 8th lane as a control. The latest version of MiSeq software (only a couple days old) supposedly allows us to lower the fraction to 5%, but how successful this is remains to be seen. Base rearrangement with the GAII allows the GAII to avoid this issue. Finally, it appears that custom priming on the MiSeq is not the same as custom priming on the HiSeq and GAII. It can still be done, but the Tm requirement is much higher. Primers that work on the HiSeq may fail on the MiSeq. As long as your Tm matches or exceeds the Tm used for Illumina primers, the MiSeq should work, but the MiSeq’s different chemistry (formamide instead of heat denaturation) is less forgiving. In summary, we have a number of technical challenges that may (or may not) effect you and we want to make sure you have all the information we can give you. I want to thank the researchers and labs that have been very patient as we have struggled running their samples which led us to identify these problems. If you believe these issues have effected your data, please do not hesitate to contact me and we can discuss how to move forward.
MARCH 11, 2013Quick update from BioMicro:
JANUARY 9, 2013Happy new years to everyone. A couple new things happening in BioMicro that we want to make everyone aware of.
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ABOUT THE BIOMICRO CENTERThe MIT BioMicro Center was founded in 2000 as the core bio-fabrication and microarray processing facility at MIT. The Center is a joint endeavor between the Department of Biology, the Koch Institute for Integrative Cancer Research, the Department of Biological Engineering and the MIT Center for Environmental Health Sciences. The BioMicro Center offers a wide range of genomic services to researchers at MIT. The majority of services rendered pertain to massively parallel sequencing using the Illumina platform (both library preparation and sequencing). Commercial array processing and include both the Affymetrix Gene Chip and Agilent DNA array platforms are also part of our portfolio. Real-time PCR and Agilent BioAnalyzer services are available in the facility both as services available to researchers, as well as for quality control of microarray and sequencing samples. In addition, the Center has a presence in high-throughput screening with robotics and plate reading as well as informatics and computational support. The BioMicro Center serves the Koch Institute as the MicroArray Technologies Core and as part of the Bioinformatics and Computing Core and the MIT Center for Environmental Health Sciences as part of the Genomics and Imaging Core Experimental and analytical work done in the BioMicro Center is funded by the NIH and must be made available through the NIH's open access policy. All Koch Institute and CEHS labs must acknowledge their core grants for work done in the core with the following language.
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