BioMicroCenter:News

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (PREVIOUS NEWSLETTERS)
m (SEPTEMBER 12, 2013)
(19 intermediate revisions not shown.)
Line 1: Line 1:
{{BioMicroCenter}}
{{BioMicroCenter}}
-
== BioMicro Center News ==
+
.
 +
== Welcome to the MIT BIOMICRO CENTER ==
 +
 
{|
{|
-
|rowspan=2 valign=top style="width:60%;padding-right:10px;"|  
+
|valign=top style="width:60%;padding-right:10px;"|  
 +
== BioMicro Center News ==
 +
=== SEPTEMBER 12, 2013 ===
-
=== September 16, 2011 ===
+
I hope everyone had a great summer. A couple new things to tell you about with the start of the academic year. First, we have two new bioinformatics scientists on board. [[BioMicroCenter:BioInformaticsStaff|Dr. Duan Ma]] comes to us from the Dept of Biostatistics at Washington University as well as several years in industry where she worked on a broad variety of bioinformatics problems as well as cloud computing solutions. [[BioMicroCenter:BioInformaticsStaff|Dr. Jie Wu]] did his PhD in computational biology Cold Spring Harbor where he focused on developing algorithms for RNAseq. Both Jie and Duan are located in [http://whreis.mit.edu 68-317], so please stop by and say hello.<BR><BR>
-
Dear users,
+
One new product to tell you about is new [http://www.illumina.com/products/miseq-reagent-kit-v3.ilmn MiSeq v3 kits] which just came out. These kits increase read counts to 25m from the current 15m maximum. The kits only come in 150 and 600 nt sizes and are somewhat more expensive than the current reagents. The changes to the MiSeq do not help v2 kits and they cannot reach the 25m threshold. We will be offering both the v2 and v3 kits for a short while, but our expectation is the v2 kits will be discontinued by Illumina in the not so distant future. New pricing for the v3 kits is up on our [[BioMicroCenter:Pricing pricing site.]]<BR><BR>
-
I hope everyone had a great summer. I have a few updates for on recent changes in the BioMicro Center.
+
Also on the sequencing front, we have some evidence that the CG bias issues we were seeing on the HiSeq may not have been totally resolved. We are in the middle of implementing some significant changes in our control lane chemistry in collaboration with Illumina. These will at least allow us to monitor the situation very aggressively and detect any changes in GC bias by using a more complex mixture of samples, instead of only phiX. As I mentioned in our [[BioMicroCenter:News#APRIL_20.2C_2013|April newsletter]], if you have reason to believe your data was compromised by this issue, please come talk to us so we can help get replacement kits to get your samples rerun as soon as possible. The new control lane is in early beta but is being included on all HiSeq flowcells now. We do want to thank the Laub, Niles and XXX labs for DNA to help us in this effort.<BR><BR>
-
First, we will be running the Technology Seminar Series again this year. This seminar series is designed to showcase a different technology in the facility each month and to bring you up to date on the latest advances and future directions of the technology in the BioMicro Center. The seminar is on Tuesdays at noon in 68-181 and lunch will be provided. We have asked the companies we have invited to bring their scientists to speak (not the sales team) so you have a chance to interact with them directly. The first session will be in just under two weeks with Tecan presenting on the robotics systems we have in the core. The full schedule is being maintained on our website at http://openwetware.org/wiki/BioMicroCenter:Technology_Seminar_Series.
+
Finally, the [[BioMicroCenter:Technology_Seminar_Series|Technology Seminar Series]] will return this year. We begin next Wednesday with Wafergen talking about the Smartchip system. The seminar will be at noon in 68-180 and lunch will be provided. We’re hoping to have seminars every month throughout the year so be sure to look for them on our website.
-
Second, we are just rolling out our newest service: Automated chromatin IP. Over the past few months we have been evaluating the IP-Star technology from Diagenode. We’ve had enough success that the instrument has been purchased and we are now offering ChIP services. The IP-star takes as input sonicated crosslinked chromatin from ~5 million cells and antibody, and ends with purified DNA. The DNA can be used for either gene specific analysis or can be carried directly in to Illumina library preparation and ChIP-seq. We have validated one set of buffer conditions that have worked robustly but the system is capable of handling a broad range of alternative conditions and can even be used to test several conditions simultaneously. I do want to point out that this is still an experimental technology and remains sensitive to most of the complexities of ChIP (requiring good antibodies and chromatin preparations) so our “guarantee” of success is much more limited than most of our other technologies, but we have seen some very promising results from our early adopters.
+
=== JUNE 17, 2013 ===
-
Finally, for those of you following the Agilent BioAnalyzer saga, we’re hoping we are at the end of it now and that the high sensitivity DNA chips are back on line. We’re still cleaning up our backlog so sample processing remains slower than we would like but I’m hoping within a week we’ll be back to normal. I do want to thank the technicians in the lab who have been working extra hours on an extremely frustrating problem to get the issue resolved.  
+
First, we have a number of [[BioMicroCenter:People staffing changes]] to tell you about. The end of June will see three members of the core moving on. Ryan Abo, one of our informatics scientists, will be heading to the Dana Farber and Paraj Patel and Pierrick Millet will be returning to Northeastern. Our new co-op students, Ashley Machado and Alexander Soltoff will be starting July 1st. We are currently undertaking a job search to look for Ryan’s replacement. If you have any concerns about the personnel changes, please feel free to contact me. <BR><BR>
-
As always, this newsletter is only sent to people who have used the BioMicro Center within the past couple years, so feel free to forward this on to anyone else who might be interested.  
+
In a piece of good news, the root cause of the critical failures we have had with our MiSeq for the last month plus with homopolymer samples appear to have been identified and should be fixed today. A recent software upgrade that was supposed to improve the handling of homopolymeric samples apparently failed to install properly, resulting in a mix of pipeline versions that was unable to handle the sequences at all. I do want to thank everyone for their patience as we have struggled with this problem and assure everyone we will move through our backlog on the MiSeq as fast as we possibly can. I also want to thank the techs in the lab, especially Scott Morin, who have been working weekends to try to get as many samples through the MiSeq as possible. <BR><BR>
-
Best regards,
+
Finally, with the end of the fiscal year, our annual price adjustments are due to take effect on July 1st. You can find a complete list of the [[BioMicroCenter:PricingFY2014|new prices here]] . The largest change is a reduction in cost for HiSeq sequencing, especially for longer reads. This is associated with a significant decrease in the amount of time we will be holding data on our servers and with the recent switch from fastq + SAM file formats to retaining only BAM files. (see January 2013 notes).
-
-Stuart Levine
+
-
--
 
-
--
 
-
Stuart Levine, PhD
 
-
BioMicro Center Director                              slevine@mit.edu
 
-
Massachusetts Institute of Technology                617-452-2949
 
-
77 Massachusetts Ave. 68-304d                    (f) 617-258-xxxx
 
-
Cambridge, MA 02139                              (c) 617-312-1286
 
-
http://web.mit.edu/biomicro/
 
 +
=== APRIL 20, 2013 ===
 +
We have noticed a number of technical issues with some Illumina runs. We want to share with you to make sure you are aware of some changes and newly identified technical issues with the platform and what we are doing to correct them where we can. All of these changes are from the Illumina side and none were especially well documented (some not at all). These issues are unlikely to be limited to the BMC, so samples from elsewhere on campus or around the country may also have these issues. Please read this as it may have some impact on your analyses.  <BR><BR>
-
=== JULY 2, 2011 ===
+
Just to begin, all of these changes are subtle and not obvious in most cases directly from the sequencers. It was the rare cases that had dramatic effects that caused us to notice them. If you decide you need to have samples rerun, we will work with you to try to get Illumina to replace the reagents and to get the samples rerun. Unfortunately, there is no way we can possibly do bulk reruns of several months’ worth of studies.  <BR><BR>
-
Dear users,
+
-
I hope everyone is having a great summer. I have a few updates for on recent changes in the BioMicro Center.
+
The most concerning issue is a dropout of GC rich regions in clustering. This has been an on-again off-again issue with Illumina that we have addressed over a year ago by improvements in amplification cycling conditions and enzyme selection. Some time, several months ago (we do not have a precise window), Illumina appears to have changed the chemistry of one of their clustering components and that caused a major change in performance on GC rich areas. This can be seen as an absence of reads from very GC rich areas but, because these areas are rare in most genomes, they cannot be seen on the flowcell wide metrics. This issue is found on current HiSeq and MiSeqV2 kits but not on MiSeqV1 kits nor, we suspect, on the GAII. We have been able to address this problem by adding a brief boiling step during NaOH denaturation of the samples and have implemented this as SOP starting about two weeks ago. This drop out of regions can cause significant issues for several studies – most notably ChIP analyses – when you are comparing data from different chemistries. <BR><BR>
-
First, by popular demand we are announcing that our RNA seq sample preparation service is officially accepting samples. This service requires 100ng of high quality eukaryotic RNA as input and is based on the Illumina TruSeq RNAseq kits coupled with the SPRIworks sample preparation robot. The initial price is $350/sample, which is a little more than then price of a microarray preparation. While the service is open, we are still in the tweaking stage and we are evaluating a number of different protocols that could reduce the total RNA amount or lower the quality of the RNA needed. We do hope to have these questions resolved in the very near future, but the quality of the data we have been getting has been sufficient that we wanted to open the service up to everyone.<BR><BR>
+
A second concern is one that has been reported in the community but we have not identified on our machines – yet – where samples from a run are being observed in the following run as minor contaminants. This issue is limited to the MiSeq and HiSeq2500 (we do not have the latter) where the tubes that add sample to the flowcell are not changed. This contamination is reported to be <1% and so would not show up on our quality metrics. However, if your MiSeq analyses are being based on finding a few reads in a large pool of discarded data or you are doing a number of sequential runs, you may wish to validate your data more carefully using an alternative technique such as qPCR or sanger sequencing. There is currently no technical fix to this problem. <BR><BR>
-
Second, prices for all of our services were updated on July 1st. Many prices are decreasing. A few key things to notice:
+
A third issue has been around for a while though we had not appreciated the implications. Illumina’s newer versions of basecalling software have become less capable of handling uniform sequence (all A’s for example). In earlier versions, only 5 basepairs of variability were needed and intensities could be determined by the control lane we run on all HiSeq flowcells. Now, it appears that nt 1-25 all must have representation of all 4 bases at all positions, even with a control lane. This has always been an issue on the MISeq and we have solved it by spiking in 30%PhiX in the lane (as opposed to our normal 0.1% spike in). Similar solutions can be used on the HiSeq. Given this change, we are re-evaluating whether there is value in using the 8th lane as a control. The latest version of MiSeq software (only a couple days old) supposedly allows us to lower the fraction to 5%, but how successful this is remains to be seen. Base rearrangement with the GAII allows the GAII to avoid this issue. <BR><BR>
-
* Agilent microarray prices are dropping to be significantly lower than Affymentrix. A full experiment can cost $350/array.  
+
-
* HiSeq lane prices have decreased – particularly for paired end samples. GAII prices have also dropped a little.
+
-
* Robotics usage prices are now set in two hour blocks instead of on a “per session” basis.
+
-
* Fluidigm usage prices will be rising to help defray repair costs.
+
-
The full list can be found '''[[BioMicroCenter:Pricing|on our pricing page]]<BR><BR>
+
-
Finally, this month we have a number of personnel changes. Katie Sullivan and Justin Elliot will be returning to Northeastern with our thanks for six months of hard work, and our new co-ops Jessica Lebowitz and Kaitlyn Sanders, will be starting on July 5th. Jessica and Kaitlyn will be taking over operation of the BioAnalyzer/LabChip and other sample quality control services. In addition, we will be adding a new bioinformaticist, Dr. Huming Ding, to the Center in early July. Dr. Ding comes to us from the University of Toronto where he worked extensively with Dr. Charlie Boone on high throughput screening of genetic interactions and will be helping us establish pipelines for analyzing Illumina data. Please say hello and make them feel welcome the next time you stop by!<BR><BR>
+
Finally, it appears that custom priming on the MiSeq is not the same as custom priming on the HiSeq and GAII. It can still be done, but the Tm requirement is much higher. Primers that work on the HiSeq may fail on the MiSeq. As long as your Tm matches or exceeds the Tm used for Illumina primers, the MiSeq should work, but the MiSeq’s different chemistry (formamide instead of heat denaturation) is less forgiving. <BR><BR>
-
Best regards and have a happy Fourth of July!<BR>
+
-
-Stuart Levine
+
-
=== May 24, 2011 ===
+
In summary, we have a number of technical challenges that may (or may not) effect you and we want to make sure you have all the information we can give you. I want to thank the researchers and labs that have been very patient as we have struggled running their samples which led us to identify these problems. If you believe these issues have effected your data, please do not hesitate to contact me and we can discuss how to move forward.  <BR><BR>
-
Dear users,
+
=== MARCH 11, 2013 ===
 +
Quick update from BioMicro: <BR><BR>
 +
The [[BioMicroCenter:Wafergen|Wafergen qPCR system]] is now operational. We have done a couple pilot experiments so far and it does seem to work, if there are a few more limitations than we anticipated. We are working with Wafergen to see how many of these can be alleviated but you are more than welcome to try it out and see if it would be useful to you. They have given us quite competitive pricing that is a lot lower than the cost for the [[BioMicroCenter:Fluidigm|Fluidigm BioMark]] . Please email us if you are interested in training.
-
We have a number of updates to let you all know about that have happened in the last few months.
 
-
First, we have continued to expand our [[BioMicroCenter:Illumina Library Preparation|DNA sample preparation services]]  . Over the past few months, we have been experimenting with the Nextera sample prep kit to complement our SPRIworks service. Where the SPRIworks system uses sonicated DNA, the Nextera kit is built to handle intact genomic DNA, using a transposase to fragment the DNA and is particularly suitable for applications that use entire genomic DNA, including copy number variation and de novo and resequencing projects. In addition, we have taken advantage of recent work from the Broad to improve our library representation by modifying our amplification protocol. The Nextera service is now available through BioMicro for the same price as the SPRIworks system and includes molecular [[BioMicroCenter:Multiplex|barcoding]] of the library. For more information about the Nextera system, please email  [[BioMicroCenter:People|Ryan Sinapius]].
 
-
In the [[BioMicroCenter:Microarrays|microarray]] area, we have made significant improvements to our Agilent microarray service. First, we have upgraded our scanner to 2um resolution, which will allow scanning of Agilent’s newest 1 million feature arrays. This has been coupled with an upgrade of the scanning and analysis software that can now handle additional quality controls. In addition, we have been working with Agilent to bring down the prices of their microarrays and we will be able to offer them at a significant discount beginning in July that will bring the [[BioMicroCenter:Pricing|price]] of microarray analysis down significantly. For more information about changes in the Agilent platform, please talk with [[BioMicroCenter:People|Manlin Luo]].
+
=== JANUARY 9, 2013 ===
 +
Happy new years to everyone. A couple new things happening in BioMicro that we want to make everyone aware of. <BR><BR>
 +
First, this month begins a year long experiment in joining the BioMicro Center Informatics team and the KI Bioinformatics and Computing Core in to a single team. Our two teams have been collaborating for several years, sharing computational infrastructure, etc. but this year we will be formalizing and expanding the relationship with the goal of creating a more efficient unified core. Informatics analysis requests should still be sent to Charlie Whittaker or to myself as usual, but will be spread across the joint team based on expertise and on availability. You are also, as always, welcome to contact any of the informatics scientists directly. We hope this will allow us to reduce waiting times and to keep costs under control.  <BR><BR>
 +
During the trial period (and hopefully going forward), pricing for informatics will be available in two flavors. First, for projects needing routine work, the subsidized rate will be $70/h for all CORE members (Biology, BE, KI, CEHS). For more involved projects, we have second option to purchase a “share” of the informatics team. This is an annual commitment for a fraction of an informaticist and will cost $960/mo for an average of 4h/week of informatics support. The monthly usage levels do not have to be exact and can be used in large blocks. The hours in the share can be used with any member of the team and the informaticist can vary from project to project.  <BR><BR>
 +
Finally, and importantly, we will be changing the way we are storing Illumina sequencing data long term. In the past, we have saved the fastq, sam and bam files, along with the quality control data, in a zipped file. These zipped files now occupy over 50TB of storage which is limiting  how we are able to handle new sequencing runs. To address this, we will be deleting the fastq and sam files from the archive and storing only the binary bam and quality control files. The fastq and sam files can be regenerated rapidly from the bam files using Picard and SamTools (though reads may not be in the same order). As always, we strongly encourage you to keep your own copy of the Illumina data and use our version only as a backup. We will begin this conversion next week.
 +
If you have any concerns, please do not hesitate to contact me.
-
Finally, in response to user demand we have purchased a number of licenses for TIBCO Spotfire Analytics. Spotfire is a widely used data analysis and visualization tool. It can handle a number of clustering functions and statistical tests and has very robust graphical capabilities. If you are interested in trying out Spotfire, please contact [[BioMicroCenter:People|Stephen Goldman]].
 
-
As a reminder, this email only goes out to people who are have used the BioMicro Center within the past couple years. Please feel free to forward this message on to anyone else who might be interested.
 
-
Thank you all for your support,
 
-
-Stuart Levine
+
|valign="top"|
-
=== Jan 2011 ===
+
== ABOUT THE BIOMICRO CENTER ==
-
Dear Users,<BR><BR>
+
-
I hope everyone had a great holiday. We have a couple updates as we begin the year.<BR><BR>
+
-
One of our goals for this year is to reduce our turnaround time as much as we can. We’ve taken a couple steps in this direction (though we have a long way to go!). First, we have brought a number of additional technicians on board. Michael Gravina joins us from Alnylam and will be working on the Illumina platform. Barbara Karampalas is joining us part time to work on automation. In addition we added two new coop students, Katie Sullivan and Justin Elliott, who will be taking Eris’ place as he returns to Northeastern, and we are looking to make an additional hire in the bioinformatics area (in collaboration with the Koch Institute Bioinformatics and Computing Core). Make sure you say hello the next time you stop by.<BR><BR>
+
-
In addition to new staff, we have also upgraded some of our equipment. This week we are adding a new Caliper LabChip system. The LabChip is a high throughput version of the Agilent BioAnalyzer (which uses Caliper technology) and can process hundreds of samples in a batch. Our on-site testing with the LabChip had a significant effect on the speed at which we were able to handle quality control. In order to automate the process, we will have to increase the minimum volume of sample we accept (to 5ul). For most applications, simply diluting your samples 2 fold prior to submission will be sufficient. We will be using the LabChip to handle high sensitivity DNA and standard RNA samples while small RNA, pico RNA and protein samples will continue to be run on the BioAnalyzer.  Prices for the LabChip and the BioAnalyzer will be the same but we will be able to offer discounts on large sample submissions that are run on the Caliper. <BR><BR>
+
-
Finally, we are in the finishing stages of beta testing our new RNA-seq sample prep service.  An addition to our existing DNA sample prep, we will be able to accept submissions of total RNA for sequencing just as we do for microarrays. Our current protocol is derived from Chris Burge’s lab but we are also testing kits from NuGEN for digital gene expression (DGE) which we hope will offer microarray quality results for considerably lower costs. If you are interested in helping out and have samples you are willing to contribute, please contact Ryan Sinapius who is coordinating the effort. <BR><BR>
+
-
As a reminder, this email only goes out to people who are have used the BioMicro Center within the past couple years. Please feel free to forward this message on to anyone else who might be interested.<BR><BR>
+
-
Thank you all for your support,<BR><BR>
+
-
-Stuart Levine<BR><BR>
+
 +
The MIT BioMicro Center was founded in 2000 as the core bio-fabrication and microarray processing facility at MIT. The Center is a joint endeavor between the [http://biology.mit.edu Department of Biology], the [http://ki.mit.edu Koch Institute for Integrative Cancer Research], the [http://be.mit.edu Department of Biological Engineering] and the [http://cehs.mit.edu MIT Center for Environmental Health Sciences.] The BioMicro Center offers a wide range of genomic services to researchers at MIT. The majority of services rendered pertain to massively parallel sequencing using the Illumina Genome Analyzer (both library preparation and sequencing). Commercial array processing and include both the Affymetrix Gene Chip and Agilent DNA array platforms continues to be a significant portion of our portfolio. Real-time PCR and Agilent BioAnalyzer services are available in the facility both as services available to researchers, as well as for quality control of microarray and sequencing samples. In addition, the Center has a presence in high-throughput screening with robotics and plate reading as well as informatics and computational support. The BioMicro Center serves the [http://ki.mit.edu Koch Institute] as the [http://ki.mit.edu/sbc/microarray MicroArray Technologies Core] and as part of the [http://ki.mit.edu/sbc/bioinformatics Bioinformatics and Computing Core] and the [http://cehs.mit.edu MIT Center for Environmental Health Sciences] as part of the [http://cehs.mit.edu/facilities.html#Genomics_and_Bioinformatics_Core Genomics and Imaging Core]<BR><BR>
 +
Experimental and analytical work done in the BioMicro Center is funded by the NIH and must be made available through the NIH's open access policy. All Koch Institute and CEHS labs '''must''' acknowledge their core grants for work done in the core with the following language.
 +
* KI ''"This work was funded by the National Cancer Institute of the NIH under award P30-CA14051"''
 +
* CEHS ''"This work was funded by the National Institute of Environmental Health Sciences of the NIH under award P30-ES002109"''
-
 
+
== PUBLICATIONS ==
-
|valign="top"|
+
'''2013'''<BR><BR>
 +
<biblio>
 +
#Paper1 pmid=23662897 <!- BMC Paper->
 +
#Paper2 pmid=23657361 <!- HD Chisholm->
 +
#Paper3 pmid=23352431 <!- HD.VB Boyer->
 +
#Paper4 pmid=23630078 <!- CW.AJ Sharp->
 +
#Paper5 pmid=23523371 <!- CW Jacks->
 +
</biblio>
 +
'''2012'''<BR><BR>
 +
<biblio>
 +
#Paper1 pmid=22981692 <!-SL Boyer: Heart->
 +
#Paper2 pmid=22847430 <!-SL Saeij->
 +
#Paper3 pmid=22102570 <!-HD Chisholm->
 +
</biblio>
 +
'''2011'''<BR><BR>
 +
<biblio>
 +
#Paper1 pmid=21892155 <!-SL Sur->
 +
</biblio>
 +
'''2010'''<BR><BR>
 +
<biblio>
 +
#Paper1 pmid=20720539 <!-SL Young->
 +
#Paper2 pmid=20581084 <!-SL Zwaka->
 +
</biblio>
 +
'''2009'''<BR><BR>
 +
<biblio>
 +
#Paper1 pmid=19531355 <!-SL Amon->
 +
</biblio>
== PREVIOUS NEWSLETTERS ==
== PREVIOUS NEWSLETTERS ==
-
 
+
'''[[BioMicroCenter:News2012|2012]]'''<BR>
'''[[BioMicroCenter:News2011|2011]]'''<BR>
'''[[BioMicroCenter:News2011|2011]]'''<BR>
'''[[BioMicroCenter:News2010|2010]]'''
'''[[BioMicroCenter:News2010|2010]]'''

Revision as of 11:36, 12 September 2013

Image:BioMicroCenter-header6.jpg

.

Contents

Welcome to the MIT BIOMICRO CENTER

BioMicro Center News

SEPTEMBER 12, 2013

I hope everyone had a great summer. A couple new things to tell you about with the start of the academic year. First, we have two new bioinformatics scientists on board. Dr. Duan Ma comes to us from the Dept of Biostatistics at Washington University as well as several years in industry where she worked on a broad variety of bioinformatics problems as well as cloud computing solutions. Dr. Jie Wu did his PhD in computational biology Cold Spring Harbor where he focused on developing algorithms for RNAseq. Both Jie and Duan are located in 68-317, so please stop by and say hello.

One new product to tell you about is new MiSeq v3 kits which just came out. These kits increase read counts to 25m from the current 15m maximum. The kits only come in 150 and 600 nt sizes and are somewhat more expensive than the current reagents. The changes to the MiSeq do not help v2 kits and they cannot reach the 25m threshold. We will be offering both the v2 and v3 kits for a short while, but our expectation is the v2 kits will be discontinued by Illumina in the not so distant future. New pricing for the v3 kits is up on our BioMicroCenter:Pricing pricing site.

Also on the sequencing front, we have some evidence that the CG bias issues we were seeing on the HiSeq may not have been totally resolved. We are in the middle of implementing some significant changes in our control lane chemistry in collaboration with Illumina. These will at least allow us to monitor the situation very aggressively and detect any changes in GC bias by using a more complex mixture of samples, instead of only phiX. As I mentioned in our April newsletter, if you have reason to believe your data was compromised by this issue, please come talk to us so we can help get replacement kits to get your samples rerun as soon as possible. The new control lane is in early beta but is being included on all HiSeq flowcells now. We do want to thank the Laub, Niles and XXX labs for DNA to help us in this effort.

Finally, the Technology Seminar Series will return this year. We begin next Wednesday with Wafergen talking about the Smartchip system. The seminar will be at noon in 68-180 and lunch will be provided. We’re hoping to have seminars every month throughout the year so be sure to look for them on our website.

JUNE 17, 2013

First, we have a number of BioMicroCenter:People staffing changes to tell you about. The end of June will see three members of the core moving on. Ryan Abo, one of our informatics scientists, will be heading to the Dana Farber and Paraj Patel and Pierrick Millet will be returning to Northeastern. Our new co-op students, Ashley Machado and Alexander Soltoff will be starting July 1st. We are currently undertaking a job search to look for Ryan’s replacement. If you have any concerns about the personnel changes, please feel free to contact me.

In a piece of good news, the root cause of the critical failures we have had with our MiSeq for the last month plus with homopolymer samples appear to have been identified and should be fixed today. A recent software upgrade that was supposed to improve the handling of homopolymeric samples apparently failed to install properly, resulting in a mix of pipeline versions that was unable to handle the sequences at all. I do want to thank everyone for their patience as we have struggled with this problem and assure everyone we will move through our backlog on the MiSeq as fast as we possibly can. I also want to thank the techs in the lab, especially Scott Morin, who have been working weekends to try to get as many samples through the MiSeq as possible.

Finally, with the end of the fiscal year, our annual price adjustments are due to take effect on July 1st. You can find a complete list of the new prices here . The largest change is a reduction in cost for HiSeq sequencing, especially for longer reads. This is associated with a significant decrease in the amount of time we will be holding data on our servers and with the recent switch from fastq + SAM file formats to retaining only BAM files. (see January 2013 notes).


APRIL 20, 2013

We have noticed a number of technical issues with some Illumina runs. We want to share with you to make sure you are aware of some changes and newly identified technical issues with the platform and what we are doing to correct them where we can. All of these changes are from the Illumina side and none were especially well documented (some not at all). These issues are unlikely to be limited to the BMC, so samples from elsewhere on campus or around the country may also have these issues. Please read this as it may have some impact on your analyses.

Just to begin, all of these changes are subtle and not obvious in most cases directly from the sequencers. It was the rare cases that had dramatic effects that caused us to notice them. If you decide you need to have samples rerun, we will work with you to try to get Illumina to replace the reagents and to get the samples rerun. Unfortunately, there is no way we can possibly do bulk reruns of several months’ worth of studies.

The most concerning issue is a dropout of GC rich regions in clustering. This has been an on-again off-again issue with Illumina that we have addressed over a year ago by improvements in amplification cycling conditions and enzyme selection. Some time, several months ago (we do not have a precise window), Illumina appears to have changed the chemistry of one of their clustering components and that caused a major change in performance on GC rich areas. This can be seen as an absence of reads from very GC rich areas but, because these areas are rare in most genomes, they cannot be seen on the flowcell wide metrics. This issue is found on current HiSeq and MiSeqV2 kits but not on MiSeqV1 kits nor, we suspect, on the GAII. We have been able to address this problem by adding a brief boiling step during NaOH denaturation of the samples and have implemented this as SOP starting about two weeks ago. This drop out of regions can cause significant issues for several studies – most notably ChIP analyses – when you are comparing data from different chemistries.

A second concern is one that has been reported in the community but we have not identified on our machines – yet – where samples from a run are being observed in the following run as minor contaminants. This issue is limited to the MiSeq and HiSeq2500 (we do not have the latter) where the tubes that add sample to the flowcell are not changed. This contamination is reported to be <1% and so would not show up on our quality metrics. However, if your MiSeq analyses are being based on finding a few reads in a large pool of discarded data or you are doing a number of sequential runs, you may wish to validate your data more carefully using an alternative technique such as qPCR or sanger sequencing. There is currently no technical fix to this problem.

A third issue has been around for a while though we had not appreciated the implications. Illumina’s newer versions of basecalling software have become less capable of handling uniform sequence (all A’s for example). In earlier versions, only 5 basepairs of variability were needed and intensities could be determined by the control lane we run on all HiSeq flowcells. Now, it appears that nt 1-25 all must have representation of all 4 bases at all positions, even with a control lane. This has always been an issue on the MISeq and we have solved it by spiking in 30%PhiX in the lane (as opposed to our normal 0.1% spike in). Similar solutions can be used on the HiSeq. Given this change, we are re-evaluating whether there is value in using the 8th lane as a control. The latest version of MiSeq software (only a couple days old) supposedly allows us to lower the fraction to 5%, but how successful this is remains to be seen. Base rearrangement with the GAII allows the GAII to avoid this issue.

Finally, it appears that custom priming on the MiSeq is not the same as custom priming on the HiSeq and GAII. It can still be done, but the Tm requirement is much higher. Primers that work on the HiSeq may fail on the MiSeq. As long as your Tm matches or exceeds the Tm used for Illumina primers, the MiSeq should work, but the MiSeq’s different chemistry (formamide instead of heat denaturation) is less forgiving.

In summary, we have a number of technical challenges that may (or may not) effect you and we want to make sure you have all the information we can give you. I want to thank the researchers and labs that have been very patient as we have struggled running their samples which led us to identify these problems. If you believe these issues have effected your data, please do not hesitate to contact me and we can discuss how to move forward.


MARCH 11, 2013

Quick update from BioMicro:

The Wafergen qPCR system is now operational. We have done a couple pilot experiments so far and it does seem to work, if there are a few more limitations than we anticipated. We are working with Wafergen to see how many of these can be alleviated but you are more than welcome to try it out and see if it would be useful to you. They have given us quite competitive pricing that is a lot lower than the cost for the Fluidigm BioMark . Please email us if you are interested in training.


JANUARY 9, 2013

Happy new years to everyone. A couple new things happening in BioMicro that we want to make everyone aware of.

First, this month begins a year long experiment in joining the BioMicro Center Informatics team and the KI Bioinformatics and Computing Core in to a single team. Our two teams have been collaborating for several years, sharing computational infrastructure, etc. but this year we will be formalizing and expanding the relationship with the goal of creating a more efficient unified core. Informatics analysis requests should still be sent to Charlie Whittaker or to myself as usual, but will be spread across the joint team based on expertise and on availability. You are also, as always, welcome to contact any of the informatics scientists directly. We hope this will allow us to reduce waiting times and to keep costs under control.

During the trial period (and hopefully going forward), pricing for informatics will be available in two flavors. First, for projects needing routine work, the subsidized rate will be $70/h for all CORE members (Biology, BE, KI, CEHS). For more involved projects, we have second option to purchase a “share” of the informatics team. This is an annual commitment for a fraction of an informaticist and will cost $960/mo for an average of 4h/week of informatics support. The monthly usage levels do not have to be exact and can be used in large blocks. The hours in the share can be used with any member of the team and the informaticist can vary from project to project.

Finally, and importantly, we will be changing the way we are storing Illumina sequencing data long term. In the past, we have saved the fastq, sam and bam files, along with the quality control data, in a zipped file. These zipped files now occupy over 50TB of storage which is limiting how we are able to handle new sequencing runs. To address this, we will be deleting the fastq and sam files from the archive and storing only the binary bam and quality control files. The fastq and sam files can be regenerated rapidly from the bam files using Picard and SamTools (though reads may not be in the same order). As always, we strongly encourage you to keep your own copy of the Illumina data and use our version only as a backup. We will begin this conversion next week. If you have any concerns, please do not hesitate to contact me.



ABOUT THE BIOMICRO CENTER

The MIT BioMicro Center was founded in 2000 as the core bio-fabrication and microarray processing facility at MIT. The Center is a joint endeavor between the Department of Biology, the Koch Institute for Integrative Cancer Research, the Department of Biological Engineering and the MIT Center for Environmental Health Sciences. The BioMicro Center offers a wide range of genomic services to researchers at MIT. The majority of services rendered pertain to massively parallel sequencing using the Illumina Genome Analyzer (both library preparation and sequencing). Commercial array processing and include both the Affymetrix Gene Chip and Agilent DNA array platforms continues to be a significant portion of our portfolio. Real-time PCR and Agilent BioAnalyzer services are available in the facility both as services available to researchers, as well as for quality control of microarray and sequencing samples. In addition, the Center has a presence in high-throughput screening with robotics and plate reading as well as informatics and computational support. The BioMicro Center serves the Koch Institute as the MicroArray Technologies Core and as part of the Bioinformatics and Computing Core and the MIT Center for Environmental Health Sciences as part of the Genomics and Imaging Core

Experimental and analytical work done in the BioMicro Center is funded by the NIH and must be made available through the NIH's open access policy. All Koch Institute and CEHS labs must acknowledge their core grants for work done in the core with the following language.

  • KI "This work was funded by the National Cancer Institute of the NIH under award P30-CA14051"
  • CEHS "This work was funded by the National Institute of Environmental Health Sciences of the NIH under award P30-ES002109"

PUBLICATIONS

2013

  1. Gravina MT, Lin JH, and Levine SS. . pmid:23662897. PubMed HubMed [Paper1]
  2. Kelly L, Ding H, Huang KH, Osburne MS, and Chisholm SW. . pmid:23657361. PubMed HubMed [Paper2]
  3. Klattenhoff CA, Scheuermann JC, Surface LE, Bradley RK, Fields PA, Steinhauser ML, Ding H, Butty VL, Torrey L, Haas S, Abo R, Tabebordbar M, Lee RT, Burge CB, and Boyer LA. . pmid:23352431. PubMed HubMed [Paper3]
  4. Gurtan AM, Ravi A, Rahl PB, Bosson AD, JnBaptiste CK, Bhutkar A, Whittaker CA, Young RA, and Sharp PA. . pmid:23630078. PubMed HubMed [Paper4]
  5. Snyder EL, Watanabe H, Magendantz M, Hoersch S, Chen TA, Wang DG, Crowley D, Whittaker CA, Meyerson M, Kimura S, and Jacks T. . pmid:23523371. PubMed HubMed [Paper5]
All Medline abstracts: PubMed HubMed

2012

  1. Wamstad JA, Alexander JM, Truty RM, Shrikumar A, Li F, Eilertson KE, Ding H, Wylie JN, Pico AR, Capra JA, Erwin G, Kattman SJ, Keller GM, Srivastava D, Levine SS, Pollard KS, Holloway AK, Boyer LA, and Bruneau BG. . pmid:22981692. PubMed HubMed [Paper1]
  2. Minot S, Melo MB, Li F, Lu D, Niedelman W, Levine SS, and Saeij JP. . pmid:22847430. PubMed HubMed [Paper2]
  3. Kelly L, Huang KH, Ding H, and Chisholm SW. . pmid:22102570. PubMed HubMed [Paper3]
All Medline abstracts: PubMed HubMed

2011

  1. Mellios N, Sugihara H, Castro J, Banerjee A, Le C, Kumar A, Crawford B, Strathmann J, Tropea D, Levine SS, Edbauer D, and Sur M. . pmid:21892155. PubMed HubMed [Paper1]

2010

  1. Kagey MH, Newman JJ, Bilodeau S, Zhan Y, Orlando DA, van Berkum NL, Ebmeier CC, Goossens J, Rahl PB, Levine SS, Taatjes DJ, Dekker J, and Young RA. . pmid:20720539. PubMed HubMed [Paper1]
  2. Dejosez M, Levine SS, Frampton GM, Whyte WA, Stratton SA, Barton MC, Gunaratne PH, Young RA, and Zwaka TP. . pmid:20581084. PubMed HubMed [Paper2]
All Medline abstracts: PubMed HubMed

2009

  1. Boselli M, Rock J, Unal E, Levine SS, and Amon A. . pmid:19531355. PubMed HubMed [Paper1]

PREVIOUS NEWSLETTERS

2012
2011
2010

RECENT CHANGES TO THE WEBSITE

12 April 2014

       18:07 BioMicroCenter:People (diff; hist) . .  (+1) . . Leigh J Manley (Talk | contribs) (WET LAB: )
Personal tools