BioMicroCenter:News

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== BioMicro Center News ==
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===JANUARY/FEBRUARY NEWSLETTER===
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January/February 2009 - HIGHLIGHTS
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== JANUARY 9, 2013 ==
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* New BioMicro Website at http://web.mit.edu/biomicro
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Happy new years to everyone. A couple new things happening in BioMicro that we want to make everyone aware of. <BR><BR>
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* New plate reader available for use in 68-252d.
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First, this month begins a year long experiment in joining the BioMicro Center Informatics team and the KI Bioinformatics and Computing Core in to a single team. Our two teams have been collaborating for several years, sharing computational infrastructure, etc. but this year we will be formalizing and expanding the relationship with the goal of creating a more efficient unified core. Informatics analysis requests should still be sent to Charlie Whittaker or to myself as usual, but will be spread across the joint team based on expertise and on availability. You are also, as always, welcome to contact any of the informatics scientists directly. We hope this will allow us to reduce waiting times and to keep costs under control.  <BR><BR>
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* Tecan EVO robotic training Feb 14th
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During the trial period (and hopefully going forward), pricing for informatics will be available in two flavors. First, for projects needing routine work, the subsidized rate will be $70/h for all CORE members (Biology, BE, KI, CEHS). For more involved projects, we have second option to purchase a “share” of the informatics team. This is an annual commitment for a fraction of an informaticist and will cost $960/mo for an average of 4h/week of informatics support. The monthly usage levels do not have to be exact and can be used in large blocks. The hours in the share can be used with any member of the team and the informaticist can vary from project to project. <BR><BR>
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* BioMicro Center Renovation in February
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Finally, and importantly, we will be changing the way we are storing Illumina sequencing data long term. In the past, we have saved the fastq, sam and bam files, along with the quality control data, in a zipped file. These zipped files now occupy over 50TB of storage which is limiting  how we are able to handle new sequencing runs. To address this, we will be deleting the fastq and sam files from the archive and storing only the binary bam and quality control files. The fastq and sam files can be regenerated rapidly from the bam files using Picard and SamTools (though reads may not be in the same order). As always, we strongly encourage you to keep your own copy of the Illumina data and use our version only as a backup. We will begin this conversion next week.
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If you have any concerns, please do not hesitate to contact me.
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It has been a very busy month in the BioMicro Center and we have a number of improvements to share with everyone. First, this month we have activated our new website (http://web.mit.edu/biomicro). This page has information about all of the resources available at the BioMicro Center as well as forms for download, calendars to schedule time on our machines and prices. The website is also set up as a wiki on the OpenWetWare platform to create a repository for information to help optimize experiments and to help shape the site to make it more useful to you. We are continuing to improve the site so check back often. You can [[Join|sign up for a wiki account.]]
 
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This month we also introduced the Varioskan plate reader. This plate reader can handle plates from 6 to 384 wells and can do fluorometrics, lumisetrics and photometrics at most wavelengths between 200 and 1000nm (there is some variability depending on the application). The Varioskan is also capable of doing real time kinetic experiments and has the ability to add up to three reagents to the plate. The Varioskan is currently located in 68-252d. More information, including data validation and a calendar to schedule time on the Varioskan can be found on [[BioMicroCenter:Varioskan|our website]].
 
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We are also proceeding apace with our activation of the Tecan EVO robotic liquid handler. The EVO is able to automate most routine molecular biology protocols for high-throughput. We have scheduled a training session for the EVO on February 14th with the staff from Tecan. If you are interested in learning about the EVO or the types of reactions that can be run on the machine, or in attending the training session, please contact me.
 
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Lastly, we are beginning the process of remodeling the BioMicro Center lab space to create more room. We are planning on doing this stepwise so as to have as minimal an impact on our services as possible, though access to portions of the lab may be restricted at times. The final layout is also [[BioMicroCenter:RenoPlans|on our website]] along with all of our monthly updates.
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-Stuart Levine
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== PUBLICATIONS ==
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'''2013'''<BR><BR>
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'''2012'''<BR><BR>
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<biblio>
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#Paper1 pmid=22981692 <!-SL Boyer: Heart->
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#Paper2 pmid=22847430 <!-SL Saeij->
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#Paper3 pmid=22102570 <!-HD Chisholm->
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</biblio>
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'''2011'''<BR><BR>
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<biblio>
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#Paper1 pmid=21892155 <!-SL Sur->
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</biblio>
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'''2010'''<BR><BR>
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<biblio>
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#Paper1 pmid=20720539 <!-SL Young->
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#Paper2 pmid=20581084 <!-SL Zwaka->
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</biblio>
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'''2009'''<BR><BR>
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<biblio>
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#Paper1 pmid=19531355 <!-SL Amon->
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</biblio>
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== PREVIOUS NEWSLETTERS ==
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'''[[BioMicroCenter:News2012|2012]]'''<BR>
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|valign="top" width="45%"|
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'''[[BioMicroCenter:News2011|2011]]'''<BR>
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'''[[BioMicroCenter:News2010|2010]]'''
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<br>
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===RECENT & UPCOMING CHANGES===
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== RECENT CHANGES TO THE WEBSITE ==
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{{BioMicroCenter:News/Changes/Content}}
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<B><BIG>PREVIOUS NEWSLETTERS </BIG></B>
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|'''[[BioMicroCenter:News/2009|2009]]'''
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|{{BioMicroCenter:News/2009/Content}}
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<B><BIG>RECENT CHANGES TO THE WEBSITE <\BIG><\B>
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{{BioMicroChanges}}
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Revision as of 23:16, 11 January 2013

Image:BioMicroCenter-header6.jpg

Contents

BioMicro Center News

JANUARY 9, 2013

Happy new years to everyone. A couple new things happening in BioMicro that we want to make everyone aware of.

First, this month begins a year long experiment in joining the BioMicro Center Informatics team and the KI Bioinformatics and Computing Core in to a single team. Our two teams have been collaborating for several years, sharing computational infrastructure, etc. but this year we will be formalizing and expanding the relationship with the goal of creating a more efficient unified core. Informatics analysis requests should still be sent to Charlie Whittaker or to myself as usual, but will be spread across the joint team based on expertise and on availability. You are also, as always, welcome to contact any of the informatics scientists directly. We hope this will allow us to reduce waiting times and to keep costs under control.

During the trial period (and hopefully going forward), pricing for informatics will be available in two flavors. First, for projects needing routine work, the subsidized rate will be $70/h for all CORE members (Biology, BE, KI, CEHS). For more involved projects, we have second option to purchase a “share” of the informatics team. This is an annual commitment for a fraction of an informaticist and will cost $960/mo for an average of 4h/week of informatics support. The monthly usage levels do not have to be exact and can be used in large blocks. The hours in the share can be used with any member of the team and the informaticist can vary from project to project.

Finally, and importantly, we will be changing the way we are storing Illumina sequencing data long term. In the past, we have saved the fastq, sam and bam files, along with the quality control data, in a zipped file. These zipped files now occupy over 50TB of storage which is limiting how we are able to handle new sequencing runs. To address this, we will be deleting the fastq and sam files from the archive and storing only the binary bam and quality control files. The fastq and sam files can be regenerated rapidly from the bam files using Picard and SamTools (though reads may not be in the same order). As always, we strongly encourage you to keep your own copy of the Illumina data and use our version only as a backup. We will begin this conversion next week. If you have any concerns, please do not hesitate to contact me.



PUBLICATIONS

2013

2012

  1. Wamstad JA, Alexander JM, Truty RM, Shrikumar A, Li F, Eilertson KE, Ding H, Wylie JN, Pico AR, Capra JA, Erwin G, Kattman SJ, Keller GM, Srivastava D, Levine SS, Pollard KS, Holloway AK, Boyer LA, and Bruneau BG. . pmid:22981692. PubMed HubMed [Paper1]
  2. Minot S, Melo MB, Li F, Lu D, Niedelman W, Levine SS, and Saeij JP. . pmid:22847430. PubMed HubMed [Paper2]
  3. Kelly L, Huang KH, Ding H, and Chisholm SW. . pmid:22102570. PubMed HubMed [Paper3]
All Medline abstracts: PubMed HubMed

2011

  1. Mellios N, Sugihara H, Castro J, Banerjee A, Le C, Kumar A, Crawford B, Strathmann J, Tropea D, Levine SS, Edbauer D, and Sur M. . pmid:21892155. PubMed HubMed [Paper1]

2010

  1. Kagey MH, Newman JJ, Bilodeau S, Zhan Y, Orlando DA, van Berkum NL, Ebmeier CC, Goossens J, Rahl PB, Levine SS, Taatjes DJ, Dekker J, and Young RA. . pmid:20720539. PubMed HubMed [Paper1]
  2. Dejosez M, Levine SS, Frampton GM, Whyte WA, Stratton SA, Barton MC, Gunaratne PH, Young RA, and Zwaka TP. . pmid:20581084. PubMed HubMed [Paper2]
All Medline abstracts: PubMed HubMed

2009

  1. Boselli M, Rock J, Unal E, Levine SS, and Amon A. . pmid:19531355. PubMed HubMed [Paper1]

PREVIOUS NEWSLETTERS

2012
2011
2010

RECENT CHANGES TO THE WEBSITE

12 July 2014

       11:01 BioMicroCenter:People (diff; hist) . .  (+3) . . Stuart S. Levine (Talk | contribs) (WET LAB: )
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