BioMicroCenter:News: Difference between revisions
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=== JULY 2, 2011 === | |||
Dear users, | |||
I hope everyone is having a great summer. I have a few updates for on recent changes in the BioMicro Center. | |||
First, by popular demand we are announcing that our RNA seq sample preparation service is officially accepting samples. This service requires 100ng of high quality eukaryotic RNA as input and is based on the Illumina TruSeq RNAseq kits coupled with the SPRIworks sample preparation robot. The initial price is $350/sample, which is a little more than then price of a microarray preparation. While the service is open, we are still in the tweaking stage and we are evaluating a number of different protocols that could reduce the total RNA amount or lower the quality of the RNA needed. We do hope to have these questions resolved in the very near future, but the quality of the data we have been getting has been sufficient that we wanted to open the service up to everyone.<BR><BR> | |||
Second, prices for all of our services were updated on July 1st. Many prices are decreasing. A few key things to notice: | |||
* Agilent microarray prices are dropping to be significantly lower than Affymentrix. A full experiment can cost $350/array. | |||
* HiSeq lane prices have decreased – particularly for paired end samples. GAII prices have also dropped a little. | |||
* Robotics usage prices are now set in two hour blocks instead of on a “per session” basis. | |||
* Fluidigm usage prices will be rising to help defray repair costs. | |||
The full list can be found '''[[BioMicroCenter:Pricing|on our pricing page]]<BR><BR> | |||
Finally, this month we have a number of personnel changes. Katie Sullivan and Justin Elliot will be returning to Northeastern with our thanks for six months of hard work, and our new co-ops Jessica Lebowitz and Kaitlyn Sanders, will be starting on July 5th. Jessica and Kaitlyn will be taking over operation of the BioAnalyzer/LabChip and other sample quality control services. In addition, we will be adding a new bioinformaticist, Dr. Huming Ding, to the Center in early July. Dr. Ding comes to us from the University of Toronto where he worked extensively with Dr. Charlie Boone on high throughput screening of genetic interactions and will be helping us establish pipelines for analyzing Illumina data. Please say hello and make them feel welcome the next time you stop by!<BR><BR> | |||
Best regards and have a happy Fourth of July!<BR> | |||
-Stuart Levine | |||
=== May 24, 2011 === | === May 24, 2011 === | ||
Revision as of 09:45, 2 July 2011
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BioMicro Center News
JULY 2, 2011Dear users, I hope everyone is having a great summer. I have a few updates for on recent changes in the BioMicro Center. First, by popular demand we are announcing that our RNA seq sample preparation service is officially accepting samples. This service requires 100ng of high quality eukaryotic RNA as input and is based on the Illumina TruSeq RNAseq kits coupled with the SPRIworks sample preparation robot. The initial price is $350/sample, which is a little more than then price of a microarray preparation. While the service is open, we are still in the tweaking stage and we are evaluating a number of different protocols that could reduce the total RNA amount or lower the quality of the RNA needed. We do hope to have these questions resolved in the very near future, but the quality of the data we have been getting has been sufficient that we wanted to open the service up to everyone. Second, prices for all of our services were updated on July 1st. Many prices are decreasing. A few key things to notice:
The full list can be found on our pricing page Finally, this month we have a number of personnel changes. Katie Sullivan and Justin Elliot will be returning to Northeastern with our thanks for six months of hard work, and our new co-ops Jessica Lebowitz and Kaitlyn Sanders, will be starting on July 5th. Jessica and Kaitlyn will be taking over operation of the BioAnalyzer/LabChip and other sample quality control services. In addition, we will be adding a new bioinformaticist, Dr. Huming Ding, to the Center in early July. Dr. Ding comes to us from the University of Toronto where he worked extensively with Dr. Charlie Boone on high throughput screening of genetic interactions and will be helping us establish pipelines for analyzing Illumina data. Please say hello and make them feel welcome the next time you stop by!
May 24, 2011Dear users, We have a number of updates to let you all know about that have happened in the last few months. First, we have continued to expand our DNA sample preparation services . Over the past few months, we have been experimenting with the Nextera sample prep kit to complement our SPRIworks service. Where the SPRIworks system uses sonicated DNA, the Nextera kit is built to handle intact genomic DNA, using a transposase to fragment the DNA and is particularly suitable for applications that use entire genomic DNA, including copy number variation and de novo and resequencing projects. In addition, we have taken advantage of recent work from the Broad to improve our library representation by modifying our amplification protocol. The Nextera service is now available through BioMicro for the same price as the SPRIworks system and includes molecular barcoding of the library. For more information about the Nextera system, please email Ryan Sinapius. In the microarray area, we have made significant improvements to our Agilent microarray service. First, we have upgraded our scanner to 2um resolution, which will allow scanning of Agilent’s newest 1 million feature arrays. This has been coupled with an upgrade of the scanning and analysis software that can now handle additional quality controls. In addition, we have been working with Agilent to bring down the prices of their microarrays and we will be able to offer them at a significant discount beginning in July that will bring the price of microarray analysis down significantly. For more information about changes in the Agilent platform, please talk with Manlin Luo. Finally, in response to user demand we have purchased a number of licenses for TIBCO Spotfire Analytics. Spotfire is a widely used data analysis and visualization tool. It can handle a number of clustering functions and statistical tests and has very robust graphical capabilities. If you are interested in trying out Spotfire, please contact Stephen Goldman. As a reminder, this email only goes out to people who are have used the BioMicro Center within the past couple years. Please feel free to forward this message on to anyone else who might be interested. Thank you all for your support, -Stuart Levine Jan 2011Dear Users,
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