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May 13, 2010 In order to increase the space available for equipment in the BioMicro Center, we are doing a little more remodeling. Please note the following changes: The freezers have been relocated to the 304 hallway The RT-PCR machines will be moving to the 316 entry. We hope this change will make accessing the RT-PCR machines easier and will also create room for the [Tecan EVO 150], scheduled to arrive in early June. Keep an eye on this page for future updates! April 19, 2010 Starting next month, the Koch Institute and the MIT BioMicro Center will be hosting a Nanostring nCounter system on a trial basis. The nCounter system is a single molecule visualizer that quantitatively detects RNA and other nucleic acids using a hybridization system in a highly multiplex manner. The system uses color-coded molecular barcodes to digitally count nucleic acid molecules in solution. The system does not use enzymes for detecting the nucleic acids, allowing direct measurements of mRNA from a variety of input materials including degraded RNA or crude cell lysates. The instruments will be located in the BioMicro Center and will be available to all members of the MIT community. We will be using this time to gauge the level of interest in the nCounter and whether it would be a viable system to purchase. If you are interested in learning more, we will be hosting a seminar with Nanostring representatives on April 30th. The seminar will be at 11am in 68-181. Nanostring representatives will be available after the talk to discuss the instruments and specific applications. More information is in the ABSTRACT for the talk. April 1, 2010 Thanks to the generosity of a grant from the Moore Foundation to Dr. Penny Chisholm and ARRA funding to Dr. Chris Burge the BioMicro Center will be significantly expanding our sequencing capacity over the next 4-6 months. The first step of this expansion begins today with the addition of a new GAIIx and cBot from Illumina. We are working hard to get "Ryland" and "Agustus Gloop" up and running and we hope to be able to shorten our queue times very soon! March 2010 Due to an increase in the cost of Illumina reagents, increase in data file size, and the complexity of sample preps, we have had to increase, slightly, the cost of Illumina sequencing. These new rates will be effective on samples submitted after April 1, 2010. Samples already in the queue will use the current rates. ILLUMINA SEQUENCING MIT - current MIT - April 1, 2010 unit Notes Single End (36nt) $805 $860 per lane inlcudes quality control (RT-PCR or BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs. Paired End (36+36nt) $1,385 $1,450 per lane Add'l 36nt $295 $300 per lane Sample Multiplexing $0 $50 per 10 samples combined Combined over the whole order. Only applies to samples combined by BCM technicians. Repriming $200 $300 per flow cell Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing. Non-MIT rates are 30% higher for academic institutions and 50% highers for non-academic institutions. Priority is given to samples from CORE facilities.

RECENT & UPCOMING CHANGES Illumina/Solexa Sequencing -- *New* New Affymetrix labeling -- *New* Notes from the ABRF Tecan EVO high-throughput -- Training Feb 12, 2009 BioMicro Center Renovation -- Coming Feb '09 PREVIOUS NEWSLETTERS 2009 May January / February 2008 December November RECENT CHANGES TO THE WEBSITE <\BIG><\B> List of abbreviations: N This edit created a new page (also see list of new pages) m This is a minor edit b This edit was performed by a bot (±123) The page size changed by this number of bytes 18 April 2024      15:01  Pan:Who we are‎ diffhist +14‎ Taopan talk contribs      15:00  Pan:Methods‎‎ 2 changes history +456‎ [Taopan‎ (2×)]       15:00 (cur | prev) +2‎ Taopan talk contribs       14:59 (cur | prev) +454‎ Taopan talk contribs      14:56  Pan:Publications‎‎ 2 changes history +396‎ [Taopan‎ (2×)]       14:56 (cur | prev) +74‎ Taopan talk contribs       14:54 (cur | prev) +322‎ Taopan talk contribs      13:03  BioMicroCenter:Pricing‎ diffhist +166‎ Challee talk contribs      12:58  BioMicroCenter:Singular Sequencing‎‎ 2 changes history +124‎ [Challee‎ (2×)]       12:58 (cur | prev) +14‎ Challee talk contribs (→‎Things to Consider)       12:57 (cur | prev) +110‎ Challee talk contribs      12:12  BioMicroCenter:Tecan Freedom Evo‎‎ 7 changes history +1,746‎ [Noelani Kamelamela‎ (7×)]       12:12 (cur | prev) +4‎ Noelani Kamelamela talk contribs       12:12 (cur | prev) +3‎ Noelani Kamelamela talk contribs       10:13 (cur | prev) +7‎ Noelani Kamelamela talk contribs (→‎verrity Chemagic 360)       10:08 (cur | prev) −42‎ Noelani Kamelamela talk contribs (→‎verrity Chemagic 360)       10:08 (cur | prev) +86‎ Noelani Kamelamela talk contribs (→‎verrity Chemagic 360)       09:34 (cur | prev) +23‎ Noelani Kamelamela talk contribs (→‎verrity Chemagic 360)       09:32 (cur | prev) +1,665‎ Noelani Kamelamela talk contribs      11:42  3D Cell Culture - McLean Taggart, Emma Villares, Maximillian Marek, Scott LeBlanc, Adam Lyons and Jacob Belden‎ diffhist −3‎ Sarah L. 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