Nanostring nCounter SystemKoch Institute and the MIT BioMicro Center is hosting a Nanostring nCounter system on a trial basis. The nCounter system is a single molecule visualizer that quantitatively detects RNA and other nucleic acids using a hybridization system in a highly multiplexed manner. The system uses color-coded molecular barcodes to digitally count nucleic acid molecules in solution. The system does not use enzymes for detecting the nucleic acids, allowing direct measurements of mRNA from a variety of input materials including degraded RNA or crude cell lysates, and is amplification free.
The instruments are located in the BioMicro Center and will be available to all members of the MIT community. We will be using the rest of this calendar year, 2010, to gauge the level of interest in the nCounter and whether it would be a viable system to purchase. If you are interested in learning more, please contact Stuart Levine
Code Setsvirtual codesets (e.g. genes involved in angiogenesis or apoptosis)or can create custom code sets.
qPCR. A study (Materna et al., 2010) on the prevalence of 172 transcription factors and signaling molecules in early sea urchin development was done using the Nanostring nCounter; a subset of these were compared to qPCR. The expression profiles created using nCounter were mostly in agreement with the qPCR results, if a significant difference in prevalence was observed the nCounter usually provided higher prevalence results.
Pricing and Schedules for the Nanostring are forthcoming. We anticipate the price of the run will be minimal ($10-$30 per sample) and your primary cost will be the Code Sets.