BioMicroCenter:NanoDrop ND-1000: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 1: | Line 1: | ||
{{BioMicroCenter}} | {{BioMicroCenter}} | ||
[[Image:Nd-1000.jpg|right|200px]] | |||
== NanoDrop ND-1000 == | == NanoDrop ND-1000 == | ||
Line 16: | Line 18: | ||
* Can determine cell density of microbial cultures in suspension | * Can determine cell density of microbial cultures in suspension | ||
== Location == | |||
The NanoDrop ND-1000 is located at the BMC, building 68 room 316 and is FREE for core department use |
Revision as of 14:51, 12 January 2009
HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
NanoDrop ND-1000
The NanoDrop ND-1000 is a full-spectrum (220 - 750nm) spectrophotometer that measures 1ul samples without the use of cuvettes or the need for dilution.
Applications
- Concentration and quality measurements of nucleic acids (DNA, RNA, oligos, PCR product, and hybridization probes). Range of 2ng/ul to 3700ng/ul (dsDNA) but has shown to be less reliable at lower concentrations.
- Measures nucleic acid probe and dye concentration to help determine microarray labeling efficiency (Cy3, Cy5, and Alexaflour dyes)
- Concentration and quality measurements of labeled and unlabeled proteins (Bradford, BCA, and Lowry assays). Range of 0.1mg/ml to 100mg/ml (BSA)
- Can perform full-spectrum UV/Vis spectrophotometry (220nm - 750nm)
- Can determine cell density of microbial cultures in suspension
Location
The NanoDrop ND-1000 is located at the BMC, building 68 room 316 and is FREE for core department use