BioMicroCenter:Multiplexed Sequencing: Difference between revisions
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The kit provided by Illumina supplies 12 oligos containing unique barcodes 6nt in length in addition to the standard primers used for sequencing, this kit can be purchased directly from Illumina or through the BioMicro Center for $2,880.00 plus shipping costs. To request an order or for more information please contact [[BioMicroCenter:People|Ali Perrotta]] or visit: http://www.illumina.com/downloads/SQ_Indexing_770-2008-011.pdf | The kit provided by Illumina supplies 12 oligos containing unique barcodes 6nt in length in addition to the standard primers used for sequencing, this kit can be purchased directly from Illumina or through the BioMicro Center for $2,880.00 plus shipping costs. To request an order or for more information please contact [[BioMicroCenter:People|Ali Perrotta]] or visit: http://www.illumina.com/downloads/SQ_Indexing_770-2008-011.pdf | ||
{| border=1 align="right" | |||
! ILLUMINA SEQUENCING | |||
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT | |||
!width=100| Non-MIT | |||
!width=100| unit | |||
! Notes | |||
|- | |||
| Sample Multiplexing | |||
|align="right"| $50 | |||
|align="right"| $100 | |||
|align="center"| per 10 samples | |||
| Combined over the whole order. Only applies to samples combined by BCM technicians. | |||
|- | |||
| Add'l Repriming | |||
|align="right"| $300 | |||
|align="right"| $600 | |||
|align="center"| per flow cell | |||
| Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing. | |||
|} | |||
<BR> | |||
Revision as of 17:09, 31 March 2010
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Multiplexed Sequencing
Multiplexed sequencing is a process of applying a "tag" or "barcode" to samples during sample preparation, allowing up to 12 samples to be run in the same lane. The tags are read out as a second read following repriming using the PEM. The samples are differentiated during the pipeline analysis. This application can be useful when studying samples that need less then 3-6 million reads per sample by helping to decrease the cost and time of sequencing.
The kit provided by Illumina supplies 12 oligos containing unique barcodes 6nt in length in addition to the standard primers used for sequencing, this kit can be purchased directly from Illumina or through the BioMicro Center for $2,880.00 plus shipping costs. To request an order or for more information please contact Ali Perrotta or visit: http://www.illumina.com/downloads/SQ_Indexing_770-2008-011.pdf
| ILLUMINA SEQUENCING | CORE LAB/MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|
| Sample Multiplexing | $50 | $100 | per 10 samples | Combined over the whole order. Only applies to samples combined by BCM technicians. |
| Add'l Repriming | $300 | $600 | per flow cell | Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing. |
