BioMicroCenter:Illumina Library Preparation - Revision history

Kevin K. Thai: /* Submission */

2012-07-02T14:27:11Z

Submission

←Older revision		Revision as of 14:27, 2 July 2012
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	Unless otherwise instructed, the BMC reserves 50% of submitted DNA in case of failures during sample prep. If you would like us to utilize the full sample, please indicate it in the notes section. <br><br>		Unless otherwise instructed, the BMC reserves 50% of submitted DNA in case of failures during sample prep. If you would like us to utilize the full sample, please indicate it in the notes section. <br><br>

-	'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you ~~elect~~ the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.	+	'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you select the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.

	'''RNA''' – Total RNA samples must be submitted in water or TE. The quantity of total RNA needs to be between 0.1-4ug for Illumina TruSeq, 1ug-4ug for strand specific RNAseq, >100pg for Low Input RNAseq.		'''RNA''' – Total RNA samples must be submitted in water or TE. The quantity of total RNA needs to be between 0.1-4ug for Illumina TruSeq, 1ug-4ug for strand specific RNAseq, >100pg for Low Input RNAseq.

-	[[BioMicroCenter:ChIP\|'''Chromatin''']] – Sonicated chromatin must be submitted at 5 million cells per 200uL volume. Antibodies must be submitted at the same time(at least 3ug of antibody per IP). Chromatin should be prepared on the SAME DAY as the ChIP whenever possible. Full details about isolation methods can be found on the [[BioMicroCenter:ChIP\|chromatin IP page]].	+	[[BioMicroCenter:ChIP\|'''Chromatin''']] – Sonicated chromatin must be submitted at 5-7 million cells per 200uL volume. Antibodies must be submitted at the same time(at least 3ug of antibody per IP). Chromatin should be prepared on the SAME DAY as the ChIP whenever possible. Full details about isolation methods can be found on the [[BioMicroCenter:ChIP\|chromatin IP page]].

	'''[[BioMicroCenter:Forms#Downloads\|Sample Submission Forms]]'''<br><br><br>		'''[[BioMicroCenter:Forms#Downloads\|Sample Submission Forms]]'''<br><br><br>

Stuart S. Levine: /* Sample Prep Services */

2012-06-20T01:13:09Z

Sample Prep Services

←Older revision		Revision as of 01:13, 20 June 2012
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	==Sample Prep Services==		==Sample Prep Services==
	=== DNA ===		=== DNA ===
-	There are ~~three~~ options when submitting DNA for library prep:	+	There are two options when submitting DNA for library prep depending on the type of sample submitted.

-	'''[[BioMicroCenter:DNA Sample Preparation\|~~BMC~~ Fragmented DNA ~~sample prep (SPRI and enrichment)~~]]''' - Samples will be pre-QC'd by [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]], run on the [[BioMicroCenter:SPRI-Works\|SPRI-works]] system using BioMicro Center adapters (with size-selection at the user's option), enriched using BioMicro Center PCR primers (including molecular barcodes for multiplexing), final-QC'd, and either submitted for Illumina sequencing or returned to the user for their own sequencing.	+	'''[[BioMicroCenter:DNA Sample Preparation\|Fragmented DNA]]''' - Samples will be pre-QC'd by [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]], run on the [[BioMicroCenter:SPRI-Works\|SPRI-works]] system using BioMicro Center adapters (with size-selection at the user's option), enriched using BioMicro Center PCR primers (including molecular barcodes for multiplexing), final-QC'd, and either submitted for Illumina sequencing or returned to the user for their own sequencing. Users may also chose an a la carte service where only SPRI is preformed.
-		+
-	~~'''[[BioMicroCenter:SPRI-Works\|BMC Fragmented DNA sample prep (SPRI-~~only~~)]]''' - Samples will be run on the [[BioMicroCenter:~~SPRI-Works\|SPRI-works]] system using '''user-supplied''' adapter mix and then returned to the user for their own enrichment. When choosing this option, please note that samples will need to be re-submitted if you plan to sequence with us later on.	+

	'''[[BioMicroCenter:Nextera\|BMC Intact DNA sample prep (NEXTERA)]]''' - Samples will be pre-QC'd by Nanodrop, processed using the Epicentre Nextera kit, enriched using Nextera PCR primers (including molecular barcodes if requested), final-QC'd on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]] and either submitted for Illumina sequencing or returned to the user for their own sequencing. At least 50ng of DNA is required for Nextera. Samples for Nextera cannot be pre-QC'ed on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]] because the large fragments will clog the microchannels.		'''[[BioMicroCenter:Nextera\|BMC Intact DNA sample prep (NEXTERA)]]''' - Samples will be pre-QC'd by Nanodrop, processed using the Epicentre Nextera kit, enriched using Nextera PCR primers (including molecular barcodes if requested), final-QC'd on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]] and either submitted for Illumina sequencing or returned to the user for their own sequencing. At least 50ng of DNA is required for Nextera. Samples for Nextera cannot be pre-QC'ed on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]] because the large fragments will clog the microchannels.

-	'''[[BioMicroCenter:PippinPrep\|Size Selection]]''' - For samples requiring tight size selection, amplified libraries can be size selected using the PippinPrep prior to sequencing.	+	'''[[BioMicroCenter:PippinPrep\|Size Selection]]''' - For samples requiring tight size selection, amplified libraries can be size selected using the [[BioMicroCenter:PippinPrep\|PippinPrep]] prior to sequencing.

	=== [[BioMicroCenter:RNAseq\|RNA]] ===		=== [[BioMicroCenter:RNAseq\|RNA]] ===
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	'''Low Input mRNA-seq''' - Samples will be pre-QC'd on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]], poly-A purified and converted to cDNA using the Ultra Low Input mRNAseq Kit from Clontech, run on the [[BioMicroCenter:SPRI-Works\|SPRI-works]] system using BioMicro Center adapters, enriched using BioMicro Center PCR primers, and submitted for Illumina sequencing.		'''Low Input mRNA-seq''' - Samples will be pre-QC'd on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]], poly-A purified and converted to cDNA using the Ultra Low Input mRNAseq Kit from Clontech, run on the [[BioMicroCenter:SPRI-Works\|SPRI-works]] system using BioMicro Center adapters, enriched using BioMicro Center PCR primers, and submitted for Illumina sequencing.

-	=== Chromatin ===	+	=== [[BioMicroCenter:ChIP\|\|Chromatin]] ===
-		+
-	'''ChIP-Seq''': Sonicated chromatin will undergo IP on the IPstar. RNase A, Proteinase K, Phenol Chloroform extraction and purification will be performed off of the instrument and submitted for QC on the Bioanalyzer. These ChIP samples may be picked up for qPCR or sent directly to SPRI for library construction and sequencing (options are listed on submission forms). Users will be contacted if ChIP samples fail QC. These samples will not be submitted to SPRI-works but will be charged for the IP.	+
-		+

		+	'''ChIP-Seq''': Sonicated chromatin will undergo IP on the IPstar. RNase A, Proteinase K, Phenol:Chloroform extraction and purification will be performed off of the instrument and submitted for QC on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]]. These ChIP samples may be picked up for qPCR or sent directly to [[BioMicroCenter:SPRI-Works\|SPRI]] for library construction and sequencing (options are listed on submission forms). Users will be contacted if ChIP samples fail QC. These samples will not be submitted to SPRI-works but will be charged for the IP.

	== Barcoding ==		== Barcoding ==

Stuart S. Levine at 21:37, 18 June 2012

2012-06-18T21:37:54Z

←Older revision		Revision as of 21:37, 18 June 2012
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	'''[[BioMicroCenter:PippinPrep\|Size Selection]]''' - For samples requiring tight size selection, amplified libraries can be size selected using the PippinPrep prior to sequencing.		'''[[BioMicroCenter:PippinPrep\|Size Selection]]''' - For samples requiring tight size selection, amplified libraries can be size selected using the PippinPrep prior to sequencing.

-	=== RNA ===	+	=== [[BioMicroCenter:RNAseq\|RNA]] ===

	There are several options when submitting RNA for library prep. These options are largely dictated by the source of the RNA, the quality of the RNA and the amount of RNA available.In addition, strand specific information is available for some methods and tight size selection is available for all methods.		There are several options when submitting RNA for library prep. These options are largely dictated by the source of the RNA, the quality of the RNA and the amount of RNA available.In addition, strand specific information is available for some methods and tight size selection is available for all methods.
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-	'''[[BioMicroCenter:~~Forms#Downloads\|~~Sample ~~Submission Forms~~]]'''~~<br><br><br>~~	+
		+	== Barcoding ==
		+	Our '''[[BioMicroCenter:DNA Sample Preparation\|BMC Fragmented DNA sample prep (SPRI and enrichment)]]''' service includes [[BioMicroCenter:Multiplex\|molecular barcoding]] to allow sequencing of multiple samples per lane. Nextera DNA prep and RNAseq-prep may include barcoding at the user's option.

	==Submission==		==Submission==
-	Unless otherwise instructed, the BMC reserves 50% of submitted DNA in case of failures during sample prep. <br><br>	+	Unless otherwise instructed, the BMC reserves 50% of submitted DNA in case of failures during sample prep. If you would like us to utilize the full sample, please indicate it in the notes section. <br><br>

	'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you elect the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.		'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you elect the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.

		+	'''RNA''' – Total RNA samples must be submitted in water or TE. The quantity of total RNA needs to be between 0.1-4ug for Illumina TruSeq, 1ug-4ug for strand specific RNAseq, >100pg for Low Input RNAseq.

		+	[[BioMicroCenter:ChIP\|'''Chromatin''']] – Sonicated chromatin must be submitted at 5 million cells per 200uL volume. Antibodies must be submitted at the same time(at least 3ug of antibody per IP). Chromatin should be prepared on the SAME DAY as the ChIP whenever possible. Full details about isolation methods can be found on the [[BioMicroCenter:ChIP\|chromatin IP page]].

-	'''RNA''' – Total RNA samples must be submitted in water or TE. The quantity of total RNA needs to be between 0.1-4ug for Illumina TruSeq, 1ug-4ug for strand specific RNAseq, >100pg for Low Input RNAseq, all protocols require a RIN above 9.0.	+	'''[[BioMicroCenter:Forms#Downloads\|Sample Submission Forms]]'''<br><br><br>
-		+
-		+
-	'''Chromatin''' – Sonicated chromatin must be submitted at 5 million cells per 200uL volume. Antibodies must be submitted at the same time(at least 3ug of antibody per IP). We have done months of testing and highly recommend using the following sonication conditions:	+
-		+
-	~~1. Resuspend fixed cells in 10mL of~~ [[~~LB1\|LB1]] <br>~~	+
-	~~2. Rock cells at 4 degrees for 10 minutes <br>~~	+
-	~~3. Pellet cells at 4 degrees for 5 minutes at 1350g <br>~~	+
-	~~4. Remove supernatant and resuspend cells in 10mL of [[LB2\|LB2]] <br>~~	+
-	~~5. Rock cells at 4 degrees for 10 minutes <br>~~	+
-	~~6. Pellet cells at 4 degrees for 5 minutes at 1350g <br>~~	+
-	~~7. Remove supernatant and resuspend cells in a volume of [[LB3\|LB3]] where there is 5 million cells/200uL <br>~~	+
-	~~'''Example~~:~~''' Resuspend 25 million cells in 1mL of [[LB3~~\|~~LB3~~]]''''''	+
-	~~<br>~~	+
-	~~8. Sonicate cells <br>~~	+
-	~~9. Pellet debris at 4 degrees for 10 minutes at 20,000g <br>~~	+
-	~~10. Submit lysate for ChIP~~	+
-	<br>	+
-	<br>	+
-	<br>	+

	== QC ==		== QC ==
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	Traditional Illumina protocols can be found on our [[BioMicroCenter:Protocols\| protocols page]]. <br><br><br>		Traditional Illumina protocols can be found on our [[BioMicroCenter:Protocols\| protocols page]]. <br><br><br>

-	~~== Barcoding ==~~	+
-	Our '''[[BioMicroCenter:DNA Sample Preparation\|BMC Fragmented DNA sample prep (SPRI and enrichment)]]''' service includes [[BioMicroCenter:Multiplex\|molecular barcoding]] to allow sequencing of multiple samples per lane. Nextera DNA prep and RNAseq-prep may include barcoding at the user's option.	+
	<br>		<br>
	<br>		<br>

Stuart S. Levine at 21:20, 3 June 2012

2012-06-03T21:20:42Z

←Older revision		Revision as of 21:20, 3 June 2012
Line 2:		Line 2:

	[[Image:BMC_samplePrep_pipes.png\|thumb\|right\|400px\|Sample Preparation available in BioMicro]]		[[Image:BMC_samplePrep_pipes.png\|thumb\|right\|400px\|Sample Preparation available in BioMicro]]
-	Generating high-quality data on the Illumina sequencing platform requires high-quality libraries. The BMC currently offers library preparation services for ~~genomic DNA and RNA-seq~~. Prior to sequencing, all samples must pass the BioMicro Center’s [[BioMicroCenter:Sequencing_Quality_Control\|Sequencing Quality Control]] process, which verifies selection of inserts of a desired size and correct ligation of Illumina adapters.<br>Sample Prep questions can be directed to [[BioMicroCenter:People\|~~Michael Gravina and Ryan Sinapius~~]].	+	Generating high-quality data on the Illumina sequencing platform requires high-quality libraries. The BMC currently offers library preparation services for a variety of starting materials. Prior to sequencing, all samples must pass the BioMicro Center’s [[BioMicroCenter:Sequencing_Quality_Control\|Sequencing Quality Control]] process, which verifies selection of inserts of a desired size and correct ligation of Illumina adapters.<br>Sample Prep questions can be directed to [[BioMicroCenter:People\|Shmulik Motola]].
	==Sample Prep Services==		==Sample Prep Services==
	=== DNA ===		=== DNA ===
Line 12:		Line 12:

	'''[[BioMicroCenter:Nextera\|BMC Intact DNA sample prep (NEXTERA)]]''' - Samples will be pre-QC'd by Nanodrop, processed using the Epicentre Nextera kit, enriched using Nextera PCR primers (including molecular barcodes if requested), final-QC'd on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]] and either submitted for Illumina sequencing or returned to the user for their own sequencing. At least 50ng of DNA is required for Nextera. Samples for Nextera cannot be pre-QC'ed on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]] because the large fragments will clog the microchannels.		'''[[BioMicroCenter:Nextera\|BMC Intact DNA sample prep (NEXTERA)]]''' - Samples will be pre-QC'd by Nanodrop, processed using the Epicentre Nextera kit, enriched using Nextera PCR primers (including molecular barcodes if requested), final-QC'd on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]] and either submitted for Illumina sequencing or returned to the user for their own sequencing. At least 50ng of DNA is required for Nextera. Samples for Nextera cannot be pre-QC'ed on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]] because the large fragments will clog the microchannels.
		+
		+	'''[[BioMicroCenter:PippinPrep\|Size Selection]]''' - For samples requiring tight size selection, amplified libraries can be size selected using the PippinPrep prior to sequencing.

	=== RNA ===		=== RNA ===

-	There are ~~three~~ options when submitting RNA for library prep:	+	There are several options when submitting RNA for library prep. These options are largely dictated by the source of the RNA, the quality of the RNA and the amount of RNA available.In addition, strand specific information is available for some methods and tight size selection is available for all methods.

	'''mRNA-seq''' - Samples will be pre-QC'd on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]], poly-A purified and converted to cDNA using the Illumina Tru-Seq protocol, run on the [[BioMicroCenter:SPRI-Works\|SPRI-works]] system using BioMicro Center adapters, enriched using BioMicro Center PCR primers, and submitted for Illumina sequencing.		'''mRNA-seq''' - Samples will be pre-QC'd on the [[BioMicroCenter:2100BioAnalyzer\|BioAnalyzer]], poly-A purified and converted to cDNA using the Illumina Tru-Seq protocol, run on the [[BioMicroCenter:SPRI-Works\|SPRI-works]] system using BioMicro Center adapters, enriched using BioMicro Center PCR primers, and submitted for Illumina sequencing.

Stuart S. Levine at 20:09, 3 June 2012

2012-06-03T20:09:31Z

←Older revision		Revision as of 20:09, 3 June 2012
Line 1:		Line 1:
	{{BioMicroCenter}}		{{BioMicroCenter}}

-	[[Image:~~Presentationbest~~.~~jpg~~\|right\|400px]]	+	[[Image:BMC_samplePrep_pipes.png\|thumb\|right\|400px\|Sample Preparation available in BioMicro]]
	Generating high-quality data on the Illumina sequencing platform requires high-quality libraries. The BMC currently offers library preparation services for genomic DNA and RNA-seq. Prior to sequencing, all samples must pass the BioMicro Center’s [[BioMicroCenter:Sequencing_Quality_Control\|Sequencing Quality Control]] process, which verifies selection of inserts of a desired size and correct ligation of Illumina adapters.<br>Sample Prep questions can be directed to [[BioMicroCenter:People\|Michael Gravina and Ryan Sinapius]].		Generating high-quality data on the Illumina sequencing platform requires high-quality libraries. The BMC currently offers library preparation services for genomic DNA and RNA-seq. Prior to sequencing, all samples must pass the BioMicro Center’s [[BioMicroCenter:Sequencing_Quality_Control\|Sequencing Quality Control]] process, which verifies selection of inserts of a desired size and correct ligation of Illumina adapters.<br>Sample Prep questions can be directed to [[BioMicroCenter:People\|Michael Gravina and Ryan Sinapius]].
	==Sample Prep Services==		==Sample Prep Services==

Michael Gravina: /* Submission */

2012-01-11T20:24:51Z

Submission

←Older revision		Revision as of 20:24, 11 January 2012
Line 31:		Line 31:

	==Submission==		==Submission==
-	Unless otherwise instructed, the BMC reserves 50% of submitted ~~material~~ in case of failures during sample prep. <br><br>	+	Unless otherwise instructed, the BMC reserves 50% of submitted DNA in case of failures during sample prep. <br><br>

	'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you elect the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.		'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you elect the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.

Michael Gravina: /* Submission */

2012-01-11T20:24:18Z

Submission

←Older revision		Revision as of 20:24, 11 January 2012
Line 31:		Line 31:

	==Submission==		==Submission==
-	Unless otherwise instructed, the BMC reserves 50% of submitted material in case of failures during sample prep. <br>	+	Unless otherwise instructed, the BMC reserves 50% of submitted material in case of failures during sample prep. <br><br>

	'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you elect the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.		'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you elect the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.

Michael Gravina: /* Submission */

2012-01-11T20:24:07Z

Submission

←Older revision		Revision as of 20:24, 11 January 2012
Line 31:		Line 31:

	==Submission==		==Submission==
-	Unless otherwise instructed, the BMC reserves 50% of submitted material in case of failures during sample prep.	+	Unless otherwise instructed, the BMC reserves 50% of submitted material in case of failures during sample prep. <br>

	'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you elect the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.		'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you elect the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.

Michael Gravina: /* Submission */

2012-01-11T20:23:51Z

Submission

←Older revision		Revision as of 20:23, 11 January 2012
Line 31:		Line 31:

	==Submission==		==Submission==
		+	Unless otherwise instructed, the BMC reserves 50% of submitted material in case of failures during sample prep.

	'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you elect the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.		'''DNA'''– DNA samples must be submitted in either water or TE. Although the [[BioMicroCenter:SPRI-Works\|SPRIworks]] robot is not highly sensitive to the amount of DNA input, care must be taken to submit an [[BioMicroCenter:SPRI-Works\|appropriate amount of '''adapter''' to ensure efficient ligation]]. If you elect the Nextera kit instead of the SPRIworks, we will require 50ng of DNA input.

Ryan Sinapius: /* Submission */

2011-12-22T20:49:04Z

Submission

←Older revision		Revision as of 20:49, 22 December 2011
Line 36:		Line 36:


-	'''RNA''' – Total RNA samples must be submitted in water or TE. The quantity of total RNA needs to be between 0.1-4ug ~~with~~ a RIN above 9.0.	+	'''RNA''' – Total RNA samples must be submitted in water or TE. The quantity of total RNA needs to be between 0.1-4ug for Illumina TruSeq, 1ug-4ug for strand specific RNAseq, >100pg for Low Input RNAseq, all protocols require a RIN above 9.0.