BioMicroCenter:HTS Library: Difference between revisions
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{{BioMicroCenter}} | {{BioMicroCenter}} | ||
The BioMicro Center provides some support for high-throughput library preparation for Illumina sequencing. High-throughput preparations can be a cost effective way to process large numbers of samples | The BioMicro Center provides some support for high-throughput library preparation for Illumina sequencing. High-throughput preparations can be a cost effective way to process large numbers of samples. However, these preps differ from our standard library preparation in several ways: | ||
* Samples are processed in large batches only. The number of samples per batch varies based on the prep type. | * Samples are processed in large batches only. The number of samples per batch varies based on the prep type. | ||
* Failed samples are NOT repeated. For some methods, they can be repeated as standard samples for an additional charge. | * Failed samples are NOT repeated. For some methods, they can be repeated as standard samples for an additional charge. | ||
* Samples are requested to be pre-QCed and submitted in a specific format. Samples that are not submitted in this format will be subject to setup and quality control costs. | * Samples are requested to be pre-QCed and submitted in a specific format. Samples that are not submitted in this format will be subject to setup and quality control costs. | ||
==HTS LIBRARY TABLE== | ==HTS LIBRARY TABLE== | ||
{| border=1 | |||
! METHOD | |||
! Samples/batch | |||
! Volume/sample (ul) | |||
! Submission Conc. | |||
|-align=center | |||
| colspan="4" |'''DNA Methods''' | |||
|-align=center | |||
| 16S metagenome | |||
| 48 | |||
| 20 | |||
| must be Ct<20 | |||
|-align=center | |||
| Other amplicon | |||
| 48 | |||
| 20 | |||
| must be Ct<20 | |||
|-align=center | |||
| Nextera XT | |||
| 16 | |||
| 7 | |||
| 0.2ng/ul <BR>(lower amounts for short amplicon) | |||
|} | |||
===Currently Available=== | ===Currently Available=== | ||
16S 48 Xul | 16S 48 Xul |
Revision as of 06:07, 21 December 2015
HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
The BioMicro Center provides some support for high-throughput library preparation for Illumina sequencing. High-throughput preparations can be a cost effective way to process large numbers of samples. However, these preps differ from our standard library preparation in several ways:
- Samples are processed in large batches only. The number of samples per batch varies based on the prep type.
- Failed samples are NOT repeated. For some methods, they can be repeated as standard samples for an additional charge.
- Samples are requested to be pre-QCed and submitted in a specific format. Samples that are not submitted in this format will be subject to setup and quality control costs.
HTS LIBRARY TABLE
METHOD | Samples/batch | Volume/sample (ul) | Submission Conc. |
---|---|---|---|
DNA Methods | |||
16S metagenome | 48 | 20 | must be Ct<20 |
Other amplicon | 48 | 20 | must be Ct<20 |
Nextera XT | 16 | 7 | 0.2ng/ul (lower amounts for short amplicon) |
Currently Available
16S 48 Xul Amplicon 48 X ul Must be amplified using BMC jumpers NexteraXT 16 7ul 0.2ng/ul
Coming Soon
HT-3'DGE RNAseq 12 7ul 2-10ng/ul(constant)