BioMicroCenter:DNA Sample Preparation: Difference between revisions
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DNA sample preparation is performed on the SPRI-te Nucleic Acid Extractor from Beckman Coulter Genomics | SPRIworks DNA sample preparation is performed on the SPRI-te Nucleic Acid Extractor from Beckman Coulter Genomics. The SPRI-te automates end-repair, A-tailing, adapter ligation, and size-selection, saving hours of hands-on time. Samples are then amplified by PCR. <br> | ||
[[Image:DNA_prep_1.png|center]] | [[Image:DNA_prep_1.png|center]] | ||
[[Image:DNA_prep_2.png|right]]In order to test performance of the SPRI-te the same ChIP-seq sample | [[Image:DNA_prep_2.png|right]]In order to test the performance of the SPRI-te, the same ChIP-seq sample underwent preparation for Illumina sequencing by both an experienced technician and by the SPRI-te. The numbers of unique sequencing reads and significant peaks were doubled in the SPRI-te samples compared to those prepared manually. <br> | ||
SPRI-te prepared samples have produced | SPRI-te prepared samples have produced high-quality data even when sample input was below the manufacturer's recommendations. Varying amounts (200ng, 50, 12.5, 3.2, 0.8 and 0.2) of 50bp ladder from New England Biolabs were prepared on the SPRI-te and yielded similar concentrations and nearly identical traces when analyzed by BioAnalyzer. <br> | ||
[[Image:DNA_prep_3.png]] [[Image:DNA_prep_4.png]] | [[Image:DNA_prep_3.png]] [[Image:DNA_prep_4.png]] | ||
Revision as of 12:36, 28 September 2011
--Under construction--
SPRIworks DNA sample preparation is performed on the SPRI-te Nucleic Acid Extractor from Beckman Coulter Genomics. The SPRI-te automates end-repair, A-tailing, adapter ligation, and size-selection, saving hours of hands-on time. Samples are then amplified by PCR.
In order to test the performance of the SPRI-te, the same ChIP-seq sample underwent preparation for Illumina sequencing by both an experienced technician and by the SPRI-te. The numbers of unique sequencing reads and significant peaks were doubled in the SPRI-te samples compared to those prepared manually.
SPRI-te prepared samples have produced high-quality data even when sample input was below the manufacturer's recommendations. Varying amounts (200ng, 50, 12.5, 3.2, 0.8 and 0.2) of 50bp ladder from New England Biolabs were prepared on the SPRI-te and yielded similar concentrations and nearly identical traces when analyzed by BioAnalyzer.
