BioMicroCenter:ChIP Seq

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{{BioMicroCenter}}
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'''Under Construction'''
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== ChIP Seq ==
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The BioMicroCenter supports ChIP (Chromatin Immunoprecipitation) sequencing using the Illumina platform. ChIPs are used to determine the presence of specific protein-DNA interactions genome-wide. It uses engineered antibodies to IP for the epitope of interest from sonicated chromatin. The IPed material can then be used for ChIP-qPCR (if only looking at a few select binding sites) or ChIP-Seq, for a more global view.
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== Sample Submission Guidelines ==
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'''Users are required to fix their own cells and sonicate the chromatin prior to submission''' by following the corresponding Illumina [[BioMicroCenter:Protocols| Protocols]].  Our standard sonication protocol can be found at  [[BioMicroCenter:ChIP|ChIP Conditions]]
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[[BioMicroCenter:Forms#Downloads|'''Sample Submission Forms''']]
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Current revision

Under Construction

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