BioMicroCenter:BorealAurora: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
 
Line 2: Line 2:


[[Image:BMC_AuroraMachine.png|thumb|right|400xp|Boreal Aurora]]
[[Image:BMC_AuroraMachine.png|thumb|right|400xp|Boreal Aurora]]
== Boreal Aurora ==
== [http://www.borealgenomics.com/aurora/system/ Boreal Aurora] ==
''' The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services'''<BR>
''' The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services'''<BR>
The Boreal Aurora system is a DNA/RNA purification system designed to remove contaminants and concentrate DNA & RNA samples. The Aurora system claims 100x contamination reduction and up to 100x sample concentration. The Aurora takes 4h-1d to run and can process samples between 300bp and 1.1Mbp of DNA and 500bp to 1.1Mbp for RNA.
The Boreal Aurora system is a DNA/RNA purification system designed to remove contaminants and concentrate DNA & RNA samples. The Aurora system claims 100x contamination reduction and up to 100x sample concentration. The Aurora takes 4h-1d to run and can process samples between 300bp and 1.1Mbp of DNA and 500bp to 1.1Mbp for RNA.


===POSSIBLE APPLICATIONS===
===[http://www.borealgenomics.com/aurora/applications/ POSSIBLE APPLICATIONS]===
* Ultra-high-MW DNA from cell culture / agarose plugs
* Ultra-high-MW DNA from cell culture / agarose plugs
* purification from low copy number bacterial samples
* purification from low copy number bacterial samples


===SCODA===
===[http://www.borealgenomics.com/aurora/technology/ SCODA]===
The Boreal Aurora works using "synchronous coefficient of drag alteration" or SCODA. SCODA uses a linear electric field to remove contaminants and a rotating electric field to concentrate charged molecules in a centeral buffer well.  
The Boreal Aurora works using "synchronous coefficient of drag alteration" or SCODA. SCODA uses a linear electric field to remove contaminants and a rotating electric field to concentrate charged molecules in a centeral buffer well.  



Latest revision as of 09:16, 28 May 2013

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY
Boreal Aurora

Boreal Aurora

The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services
The Boreal Aurora system is a DNA/RNA purification system designed to remove contaminants and concentrate DNA & RNA samples. The Aurora system claims 100x contamination reduction and up to 100x sample concentration. The Aurora takes 4h-1d to run and can process samples between 300bp and 1.1Mbp of DNA and 500bp to 1.1Mbp for RNA.

POSSIBLE APPLICATIONS

  • Ultra-high-MW DNA from cell culture / agarose plugs
  • purification from low copy number bacterial samples

SCODA

The Boreal Aurora works using "synchronous coefficient of drag alteration" or SCODA. SCODA uses a linear electric field to remove contaminants and a rotating electric field to concentrate charged molecules in a centeral buffer well.

LIMITS

SCODA Chip

Samples must be very low salt to be successful and within the size range of the machine (300bp-1.1Mbp). Up to 5ml can be loaded. The machine can only handle one sample at a time so has quite limited throughput.