BioMicroCenter:BorealAurora

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(New page: {{BioMicroCenter}} == Boreal Aurora == ''' The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services'''<BR> The Boreal Aur...)
(Boreal Aurora)
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{{BioMicroCenter}}
{{BioMicroCenter}}
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[[Image:BMC_AuroraMachine.png|thumb|right|400xp|Boreal Aurora]]
== Boreal Aurora ==
== Boreal Aurora ==
''' The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services'''<BR>
''' The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services'''<BR>
The Boreal Aurora system is a DNA/RNA purification system designed to remove contaminants and concentrate DNA & RNA samples. The Aurora system claims 100x contamination reduction and up to 100x sample concentration. The Aurora takes 4h-1d to run and can process samples between 300bp and 1.1Mbp of DNA and 500bp to 1.1Mbp for RNA.
The Boreal Aurora system is a DNA/RNA purification system designed to remove contaminants and concentrate DNA & RNA samples. The Aurora system claims 100x contamination reduction and up to 100x sample concentration. The Aurora takes 4h-1d to run and can process samples between 300bp and 1.1Mbp of DNA and 500bp to 1.1Mbp for RNA.
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===Possible Applications===
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===POSSIBLE APPLICATIONS===
* Ultra-high-MW DNA from cell culture / agarose plugs
* Ultra-high-MW DNA from cell culture / agarose plugs
* purification from low copy number bacterial samples
* purification from low copy number bacterial samples
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===LIMITS===
===LIMITS===
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Samples must be very low salt to be successful and within the size range of the machine (300bp-1.1Mbp). The machine can only handle one sample at a time so has quite limited throughput.
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[[Image:BMC_AuroraCHIP.png|thumb|right|200px|SCODA Chip]]
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Samples must be very low salt to be successful and within the size range of the machine (300bp-1.1Mbp). Up to 5ml can be loaded. The machine can only handle one sample at a time so has quite limited throughput.

Revision as of 11:28, 28 May 2013

Image:BioMicroCenter-header6.jpg

Boreal Aurora
Boreal Aurora

Contents

Boreal Aurora

The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services
The Boreal Aurora system is a DNA/RNA purification system designed to remove contaminants and concentrate DNA & RNA samples. The Aurora system claims 100x contamination reduction and up to 100x sample concentration. The Aurora takes 4h-1d to run and can process samples between 300bp and 1.1Mbp of DNA and 500bp to 1.1Mbp for RNA.

POSSIBLE APPLICATIONS

  • Ultra-high-MW DNA from cell culture / agarose plugs
  • purification from low copy number bacterial samples

SCODA

The Boreal Aurora works using "synchronous coefficient of drag alteration" or SCODA. SCODA uses a linear electric field to remove contaminants and a rotating electric field to concentrate charged molecules in a centeral buffer well.

LIMITS

SCODA Chip
SCODA Chip

Samples must be very low salt to be successful and within the size range of the machine (300bp-1.1Mbp). Up to 5ml can be loaded. The machine can only handle one sample at a time so has quite limited throughput.

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