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Lab 5: Golden Bread
- Engineering reliability into an unstable genetic system may or may not make it a robust and profitable food source.
Acknowledgments: This lab was developed with materials from the Johns Hopkins 2011 iGEM team, as well as guidance and technical insights from BioBuilder teachers around the country
Objectives
By the conclusion of this laboratory investigation, the student will be able to:
- Define and properly use synthetic biology terms: chassis, system, device, redundancy
- Define and properly use molecular genetics terms: PCR, gene expression, codon shuffling.
- Explain the role of redundancy in synthetic biology and engineering.
- Conduct PCR and TLC and interpret the results each.
- Compare two engineering solutions to a given problem (redundancy vs kill switches)
Introduction
"Nature is a masterful and prolific chemist" [http://mmbr.asm.org/content/69/1/51.short
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