BioBuilding: Synthetic Biology for Students: Lab 1: Difference between revisions
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* Culture bacteria using proper microbiology methods. | * Culture bacteria using proper microbiology methods. | ||
* Measure the growth of a bacterial population. | * Measure the growth of a bacterial population. | ||
* Define and properly use synthetic biology terms: [[http://youtu.be/X7FUTCvmiE8 | * Define and properly use synthetic biology terms: [[http://youtu.be/X7FUTCvmiE8 Part,]][[http://youtu.be/aPqqS8vIAKA Device,]] [[http://youtu.be/ER2wz3I-2KU Inverter.]] | ||
* Define and properly use molecular genetics terms: [[http://youtu.be/8oHSRBZGMdM | * Define and properly use molecular genetics terms: [[http://youtu.be/8oHSRBZGMdM Promoter,]][[http://youtu.be/8oHSRBZGMdM ribosome binding site ("RBS"),]] [[http://youtu.be/2x8pkL6bAeQ open reading frame ("ORF"),]] [[http://youtu.be/8oHSRBZGMdM Terminator,]] [[http://openwetware.org/wiki/BioBuilding:_Synthetic_Biology_for_Students:_Glossary Plasmid.]] | ||
==Introduction== | ==Introduction== |
Revision as of 19:18, 24 July 2011
Eau That Smell Lab |
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LAB 1: Eau that smell
Acknowledgments: This lab was developed with materials and guidance from the MIT 2006 iGEM team, as well as technical insights and help from Ginkgo BioworksObjectivesBy the conclusion of this laboratory investigation, the student will be able to:
IntroductionFor the 2006 [iGEM] competition, MIT students designed Eau d’coli, E. coli that smell like bananas when their population is in the stationary phase. They did this by inserting a device that contains a stationary phase sensitive promoter coupled to a banana smell device, a device that contains a ribosome binding site (RBS), an open reading frame (ORF) that codes for the ATF1 enzyme and terminator sequences. The ATF1 enzyme converts isoamyl alcohol to isoamyl acetate, the molecule that gives bananas their characteristic smell. It has been suggested that a device to generate the banana smell during the bacteria’s log (or exponential) phase of population growth will be helpful. There are two ways to accomplish this. Both approaches will continue to use the original banana smell device but alter the function of the promoter. One approach couples the banana smell device to a new part, a log phase promoter. The other approach uses the same promoter but adds a genetic inverter. Synthetic biologists have constructed these devices for us and transformed bacteria with them. ProcedureThere are two versions of this lab. Your teacher will inform you of the one you will be doing. Click on the links below to go to the detailed procedure: Lab ReportAs you write, be sure to define and properly use all highlighted terms throughout the introduction and other parts of the lab. I. Introduction
II. Methods
III. Results
IV. Discussion
V. Citations and references
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