BioBuilding: Synthetic Biology for Students: Lab 1: Difference between revisions
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==Introduction== | ==Introduction== | ||
[[Image:Intro lab1 BioPrimer1.png|thumb|600 px| [ | [[Image:Intro lab1 BioPrimer1.png|thumb|600 px| [Image:Bioprimer no1 02.02.jpg BioPrimer #1 pdf] ]] | ||
For the 2006 [[http://youtu.be/5HXrrN7mV0A| iGEM]] competition, MIT students designed Eau d’coli, ''E. coli'' that smell like bananas when their population is in the stationary phase. They did this by inserting device that contains a stationary phase sensitive promoter coupled to a banana smell device, a device that contains a ribosome binding site (RBS), an open reading frame (ORF) that codes for the ATF1 enzyme and terminator sequences. The ATF1 enzyme converts isoamyl alcohol to isoamyl acetate, the molecule that gives bananas their characteristic smell. | For the 2006 [[http://youtu.be/5HXrrN7mV0A| iGEM]] competition, MIT students designed Eau d’coli, ''E. coli'' that smell like bananas when their population is in the stationary phase. They did this by inserting device that contains a stationary phase sensitive promoter coupled to a banana smell device, a device that contains a ribosome binding site (RBS), an open reading frame (ORF) that codes for the ATF1 enzyme and terminator sequences. The ATF1 enzyme converts isoamyl alcohol to isoamyl acetate, the molecule that gives bananas their characteristic smell. | ||
Revision as of 22:11, 22 July 2011
Eau That Smell Lab |
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LAB 1: Eau that smell
Acknowledgments: This lab was developed with materials and guidance from the MIT 2006 iGEM team, as well as technical insights and help from Ginkgo BioworksObjectivesBy the conclusion of this laboratory investigation, the student will be able to:
IntroductionFor the 2006 [iGEM] competition, MIT students designed Eau d’coli, E. coli that smell like bananas when their population is in the stationary phase. They did this by inserting device that contains a stationary phase sensitive promoter coupled to a banana smell device, a device that contains a ribosome binding site (RBS), an open reading frame (ORF) that codes for the ATF1 enzyme and terminator sequences. The ATF1 enzyme converts isoamyl alcohol to isoamyl acetate, the molecule that gives bananas their characteristic smell. It has been suggested that a device that generates the banana smell during the bacteria’s log (or exponential) phase of population growth will be helpful. There are two ways to accomplish this. Both methods will continue to use the banana smell device but alter the function of the promoter. One method involves coupling the banana smell device to a new part, a log phase promoter. The other method involves using the same promoter but adding an inverter. Synthetic biologists have constructed these devices for us and transformed bacteria with them. ProcedureThere are two versions of this lab. Your teacher will inform you of the one you will be doing. Click on the links below to go to the detailed procedure: Lab ReportAs you write, be sure to define and properly use all highlighted terms throughout the introduction and other parts of the lab. I. Introduction
II. Methods
III. Results
IV. Discussion
V. Citations and references
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