BioBuilding: Synthetic Biology for Students: Lab 1: Difference between revisions
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* Measure the growth of a bacterial population. | * Measure the growth of a bacterial population. | ||
* Define and properly use synthetic biology terms: [[http://youtu.be/X7FUTCvmiE8| Part,]][[http://youtu.be/aPqqS8vIAKA| Device,]] [[http://youtu.be/ER2wz3I-2KU| Inverter.]] | * Define and properly use synthetic biology terms: [[http://youtu.be/X7FUTCvmiE8| Part,]][[http://youtu.be/aPqqS8vIAKA| Device,]] [[http://youtu.be/ER2wz3I-2KU| Inverter.]] | ||
* Define and properly use molecular genetics terms: [[http://youtu.be/8oHSRBZGMdM| Promoter,]][[http://youtu.be/8oHSRBZGMdM| ribosome binding site ("RBS"),]] [[http://youtu.be/2x8pkL6bAeQ| open reading frame ("ORF"),] [http://youtu.be/8oHSRBZGMdM| Terminator,]] [[http://openwetware.org/wiki/BioBuilding:_Synthetic_Biology_for_Students:_Glossary| Plasmid.]] | * Define and properly use molecular genetics terms: [[http://youtu.be/8oHSRBZGMdM| Promoter,]][[http://youtu.be/8oHSRBZGMdM| ribosome binding site ("RBS"),]] [[http://youtu.be/2x8pkL6bAeQ| open reading frame ("ORF"),]] [[http://youtu.be/8oHSRBZGMdM| Terminator,]] [[http://openwetware.org/wiki/BioBuilding:_Synthetic_Biology_for_Students:_Glossary| Plasmid.]] | ||
==Introduction== | ==Introduction== | ||
[[Image:Intro lab1 BioPrimer1.png|thumb|600 px| [http://openwetware.org/images/3/30/BioPrimerNo1.pdf BioPrimer #1 pdf] ]] | [[Image:Intro lab1 BioPrimer1.png|thumb|600 px| [http://openwetware.org/images/3/30/BioPrimerNo1.pdf BioPrimer #1 pdf] ]] | ||
For the 2006 [http:// | For the 2006 [http://youtu.be/5HXrrN7mV0A| iGEM]competition, MIT students designed Eau d’Coli, ''E. coli'' that smell like bananas when their population is in the stationary phase. They did this by inserting device that contains a stationary phase sensitive promoter coupled to a banana smell device, a device that contains a ribosome binding site (RBS), an open reading frame (ORF) that codes for the ATF1 enzyme and terminator sequences. The ATF1 enzyme converts isoamyl alcohol to isoamyl acetate, the molecule that gives bananas their characteristic smell. | ||
It has been suggested that a device that generates the banana smell during the bacteria’s log (or exponential) phase of population growth will be helpful. There are two ways to accomplish this. Both methods will continue to use the banana smell device but alter the function of the promoter. One method involves coupling the banana smell device to a new part, a log phase promoter. The other method involves using the same promoter but adding an inverter. Synthetic biologists have constructed these devices for us and transformed bacteria with them.<br style="clear:both" /><br> | It has been suggested that a device that generates the banana smell during the bacteria’s log (or exponential) phase of population growth will be helpful. There are two ways to accomplish this. Both methods will continue to use the banana smell device but alter the function of the promoter. One method involves coupling the banana smell device to a new part, a log phase promoter. The other method involves using the same promoter but adding an inverter. Synthetic biologists have constructed these devices for us and transformed bacteria with them.<br style="clear:both" /><br> | ||
Revision as of 22:07, 22 July 2011
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Eau That Smell Lab |
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LAB 1: Eau that smell
Acknowledgments: This lab was developed with materials and guidance from the MIT 2006 iGEM team, as well as technical insights and help from Ginkgo BioworksObjectivesBy the conclusion of this laboratory investigation, the student will be able to:
Introduction For the 2006 [http://youtu.be/5HXrrN7mV0A |
