BioBuilding: Synthetic Biology for Students: Lab 1: Difference between revisions
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Our task will be to grow these bacterial populations and test for the banana smell as the population moves through the log phase and into the stationary phase. We will determine the population growth by using a spectrophotometer or the McFarland Turbidity Standards to measure the density of the bacteria in liquid culture. As the population increases we can assess the increasing banana smell, comparing the smell to dilutions of banana extract. | Our task will be to grow these bacterial populations and test for the banana smell as the population moves through the log phase and into the stationary phase. We will determine the population growth by using a spectrophotometer or the McFarland Turbidity Standards to measure the density of the bacteria in liquid culture. As the population increases we can assess the increasing banana smell, comparing the smell to dilutions of banana extract. | ||
==Procedure== | ==Procedure== | ||
[[BioBuilding:_Synthetic_Biology_for_Students:_Lab_1_--Protocol_A | [[BioBuilding:_Synthetic_Biology_for_Students:_Lab_1_--Protocol_A |Protocol A]] | ||
[[BioBuilding:_Synthetic_Biology_for_Students:_Lab_1--Protocol_B | [[BioBuilding:_Synthetic_Biology_for_Students:_Lab_1--Protocol_B |Protocol B]] | ||
==Lab Report== | ==Lab Report== |
Revision as of 15:44, 13 July 2011
Eau That Smell Lab |
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LAB 1: Eau that smell
Acknowledgments: This lab was developed with materials and guidance from the MIT 2006 iGEM team, as well as technical insights and help from Ginkgo BioworksObjectivesBy the conclusion of this laboratory investigation, the student will be able to:
IntroductionFor the 2006 iGEMcompetition, MIT students designed Eau d’Coli, E. coli that smell like bananas when their population is in the stationary phase. They did this by inserting device that contains a stationary phase sensitive promoter coupled to a banana smell device, a device that contains a ribosome binding site (RBS), an open reading frame (ORF) that codes for the ATF1 enzyme and terminator sequences. The ATF1 enzyme converts isoamyl alcohol to isoamyl acetate, the molecule that gives bananas their characteristic smell. It has been suggested that a device that generates the banana smell during the bacteria’s log (or exponential) phase of population growth will be helpful. There are two ways to accomplish this. Both methods will continue to use the banana smell device but alter the function of the promoter. One method involves coupling the banana smell device to a new part, a log phase promoter. The other method involves using the same promoter but adding an inverter. Synthetic biologists have constructed these devices for us and transformed bacteria with them. ProcedureLab ReportAs you write, be sure to define and properly use all highlighted terms throughout the introduction and other parts of the lab. I. Introduction
II. Methods
III. Results
IV. Discussion
V. Citations and references
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