Beta-lactamase

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (13:28, 5 August 2009) (view source)
(Plasmid stabilization)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
==Colorimetric reporter for B-lac activity==
==Colorimetric reporter for B-lac activity==
 +
 +
==Plasmid stabilization==
 +
*Beta-lactamase gene ''bla'' or ''ampR'' is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and [[carbenicillin]].
NITROCEFIN (GLAXO RESEARCH 87/312)<br>
NITROCEFIN (GLAXO RESEARCH 87/312)<br>
Line 5: Line 8:
For the rapid chromogenic detection of beta-lactamase activity.<br>
For the rapid chromogenic detection of beta-lactamase activity.<br>
[http://www.oxoid.com/uk/index.asp?mpage=iproductdetail&pre=SR0112&l=EN&x= Oxiod Inc. page on Nitrocefin]
[http://www.oxoid.com/uk/index.asp?mpage=iproductdetail&pre=SR0112&l=EN&x= Oxiod Inc. page on Nitrocefin]
 +
 +
==References==
 +
<biblio>
 +
#Anderson-Lancet-1965 pmid=14238093
 +
#Datta-BiochemJ-1966 pmid=5328167
 +
#Evans-JBacteriol-1968 pmid=4971890
 +
#MolecularCloning isbn=0-87969-577-3
 +
#Gao-JACS-2003 pmid=16220906
 +
</biblio>
 +
 +
See [http://www.tsienlab.ucsd.edu/Publications/Gao%202003%20JACS%20-%20Novel%20Fluorogenic%20Substrates.pdf here] for the full article.
[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]
[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]

Current revision

Colorimetric reporter for B-lac activity

Plasmid stabilization

  • Beta-lactamase gene bla or ampR is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and carbenicillin.

NITROCEFIN (GLAXO RESEARCH 87/312)
Code: SR0112
For the rapid chromogenic detection of beta-lactamase activity.
Oxiod Inc. page on Nitrocefin

References

  1. ANDERSON ES and DATTA N. . pmid:14238093. PubMed HubMed [Anderson-Lancet-1965]
  2. Datta N and Richmond MH. . pmid:5328167. PubMed HubMed [Datta-BiochemJ-1966]
  3. Evans J, Galindo E, Olarte J, and Falkow S. . pmid:4971890. PubMed HubMed [Evans-JBacteriol-1968]
  4. isbn:0-87969-577-3. [MolecularCloning]
  5. Gao W, Xing B, Tsien RY, and Rao J. . pmid:16220906. PubMed HubMed [Gao-JACS-2003]
All Medline abstracts: PubMed HubMed

See here for the full article.

Personal tools