Beta-glucuronidase protocols

Introduction

These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols.

Plate Screen

Materials

• Media of choice
• Agar
• X-gluc stock solution (50mg/mL)

Method

1. Prepare your liquid media and add the desired amount of agar (usually 1-2%).
2. Autoclave the media for the requisite time.
3. Add 1.2μL of X-gluc stock solution for every mL of media (e.g. if making 1L of media add 1.2ml of stock solution)
4. If desired, add antibiotic.
5. Pour plates.