Beta-glucuronidase protocols: Difference between revisions
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==Plate Screen== | ==Plate Screen== | ||
===Materials=== | ===Materials=== | ||
*Media of choice | |||
*X-gluc stock solution (0.1M = 52.2mg/mL) | |||
===Method=== | ===Method=== | ||
Revision as of 22:30, 4 July 2011
Introduction
These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols.
Plate Screen
Materials
- Media of choice
- X-gluc stock solution (0.1M = 52.2mg/mL)
