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Revision as of 01:30, 5 July 2011
These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols.
- Media of choice
- X-gluc stock solution (0.1M = 52.2mg/mL)